Path: 2022-09-02_11-03-13_Two-column-separation Procedure: Two column separation Argument list: uses_trap = True uses_separator = True is_isocratic = False analysis_time = 1800 oven_temperature = 50 sample_volume = 5 sample_position = Slot2:21 presample_solvent_volume = 2 presample_air_volume = 0.5 postsample_air_volume = 1 postsample_solvent_volume = 2 sample_aspirate_speed = 5 sample_postaspirate_delay = 500 sample_inject_speed = 1 calibrant_volume = 0.6 calibrantTime = 10 is_calibrant_inject = False is_extended_wash = False is_bottom_sense = True injection_method = uLPickup trap = Name: Thermo Trap Cartridge 5mm, Func: Separator, ColDia: 0.3 mm, Len: 5 mm, PartDia: 5 µm, Porosity: 42 % trap_volume = 0.148440252882118 trap_unityflow = 2.78838695282287 trap_equilibration_volumemultiplier = 10 trap_equilibration_pressure = 217.5 trap_equil_time = 0.648440252882118 separator = Name: IonOpticks 25 cm, Func: Separator, ColDia: 0.075 mm, Len: 250 mm, PartDia: 1.6 µm, Porosity: 42 % separator_volume = 0.463875790256618 separator_unityflow = 0.00058605984562038 separator_equilibration_volumemultiplier = 4 separator_equilibration_pressure = 800 column_load_volumemultiplier = 4 column_load_pressure = 217.5 separator_equil_time = 4.05402026133927 column_load_time = 0.6 gradient = (0 sec,0.3 µL/min,0.00 %), (1800 sec,0.3 µL/min,32.00 %), (2700 sec,0.3 µL/min,40.00 %), (3000 sec,0.3 µL/min,60.00 %), (3300 sec,0.3 µL/min,95.00 %), (3600 sec,0.3 µL/min,95.00 %) 00:00:00.0 STARTED 00:00:00.8 Lua version: 1.1.0 00:00:00.8 Lua date: 2020/01/30 00:00:00.8 --- Experiment: Description 00:00:00.8 ------------------------------------------------------- 00:00:00.8 --- Trap Column: Thermo Trap Cartridge 5mm 00:00:00.8 --- Manufacturer: Thermo Scientific 00:00:00.8 --- Part Number: 00:00:00.8 --- Serial Number: 00:00:00.8 --- Type/Material: µ-Precolum TM Cartridge / PepMap TM C18 00:00:00.8 --- Length: 5 mm 00:00:00.8 --- Inner Diameter: 0.3 mm 00:00:00.8 --- Particle Diameter: 5 um 00:00:00.8 --- Is Particle Pore Size: True 00:00:00.8 --- Particle Pore Size: 100 00:00:00.8 --- Is Column Porosity: False 00:00:00.8 --- Column Porosity: 0.42 (0.0-1.0) 00:00:00.8 --- Is Max Temperature: False 00:00:00.8 --- max Temperature: 65 C 00:00:00.8 --- Is Max Pressure: False 00:00:00.8 --- max Pressure: 1000 bar 00:00:00.8 --- Is Max Flow: False 00:00:00.8 --- max Flow: 9 uL/min 00:00:00.8 --- Advanced Settings: False 00:00:00.8 --- Column Comments: Manufacturer no: 160454 00:00:00.8 ------------------------------------------------------- 00:00:00.8 --- Separation Column: IonOpticks 25 cm 00:00:00.8 --- Manufacturer: IonOpticks 00:00:00.8 --- Part Number: 00:00:00.8 --- Serial Number: 00:00:00.8 --- Type/Material: C18 00:00:00.8 --- Length: 250 mm 00:00:00.8 --- Inner Diameter: 0.075 mm 00:00:00.8 --- Particle Diameter: 1.6 um 00:00:00.8 --- Is Particle Pore Size: True 00:00:00.8 --- Particle Pore Size: 120 00:00:00.8 --- Is Column Porosity: False 00:00:00.8 --- Column Porosity: 0.42 (0.0-1.0) 00:00:00.8 --- Is Max Temperature: False 00:00:00.8 --- max Temperature: 65 C 00:00:00.8 --- Is Max Pressure: True 00:00:00.8 --- max Pressure: 1000 bar 00:00:00.8 --- Is Max Flow: False 00:00:00.8 --- max Flow: 9 uL/min 00:00:00.8 --- Advanced Settings: False 00:00:00.8 --- Column Comments: 00:00:00.8 ------------------------------------------------------- 00:00:00.8 'Valve Drive 1' is 'ILcInjectorValve' 00:00:00.8 'Valve Drive 2' is 'ISelectorValve' 00:00:00.8 --- Trap equil time: 0.64 00:00:00.8 --- Seperator equil time: 4.05 00:00:00.8 --- Column loading time: 0.6 00:00:00.8 pump: GetSettings() --> PumpSettings 00:00:00.8 -------------------------------------------------------- 00:00:00.8 --- Log instrument settings called from: Two Column Separation 00:00:00.8 -------------------------------------------------------- 00:00:00.8 --- HomeSpeed: 2000; PurgeSpeed: 2000 00:00:00.8 --- PressureTrackingExcessFactor: 96 00:00:00.8 --- FlowResistanceA: 300; FlowResistanceB: 100; FlowResistanceOut: 150 00:00:00.8 --- FC_FlowFilterDepth: 5; FC_PressureFilterDepth: 0 00:00:00.8 --- PC_OutputFilterDepth: 0 00:00:00.8 --- CompressibilityFactorB: 2.29999995231628; PressurePIDNegativeResultDivisor: 1 00:00:00.8 --- ExternalZeroPressureOffsetA: 130.617385864258; ExternalZeroPressureOffsetB: 127.978851318359 00:00:00.8 --- ExternalPressureScalingFactorA: 0.0197199992835522; ExternalPressureScalingFactorB: 0.0197199992835522 00:00:00.8 -------------------------------------------------------- 00:00:00.8 --- Oven temperature: 50 00:00:00.8 --- Separator: Name: IonOpticks 25 cm, Func: Separator, ColDia: 0.075 mm, Len: 250 mm, PartDia: 1.6 µm, Porosity: 42 % --- 00:00:00.8 --- Trap: Name: Thermo Trap Cartridge 5mm, Func: Separator, ColDia: 0.3 mm, Len: 5 mm, PartDia: 5 µm, Porosity: 42 % --- 00:00:00.8 --- MaxPressureLimitAB: 1000 bar --- 00:00:00.8 pump: GetCurrentPressure(A) --> 7.540929 00:00:00.8 pump: GetCurrentPressure(B) --> 7.911664 00:00:00.8 pump: GetSettings() --> PumpSettings 00:00:00.8 pump: GetSetManualValvePosition(A) --> 240 00:00:00.8 pump: GetSetManualValvePosition(B) --> 240 00:00:00.8 ------------------------------------------------------- 00:00:00.8 --- Reduce pressure --- 00:00:00.8 ------------------------------------------------------- 00:00:00.8 --- Pressure channel A: 7.54092931747437 00:00:00.8 --- Pressure channel B: 7.91166353225708 00:00:00.8 pump: Manualmode_Pump_constantSpeed(A, 0) 00:00:00.8 pump: GetSetPistonSpeed(A) --> 0 00:00:00.8 pump: Manualmode_Pump_constantSpeed(B, 0) 00:00:00.8 pump: GetSetPistonSpeed(B) --> 0 00:00:00.8 pump: IsIdle() --> True 00:00:00.8 ------------------------------------------------------- 00:00:00.9 pump: SetMaxPressureLimit(A, 1020) 00:00:00.9 pump: GetMaxPressureLimit(A) --> 1020 00:00:01.0 pump: SetMaxPressureLimit(B, 1020) 00:00:01.0 pump: GetMaxPressureLimit(B) --> 1020 00:00:01.0 pump: GetSettings() --> PumpSettings 00:00:01.0 ------------------------------------------------------------------ 00:00:01.0 PID settings changed: 00:00:01.0 ------------------------------------------------------------------ 00:00:01.0 P: 175 : True; Pmin: -5000 : True; Pmax: 5000 : True 00:00:01.0 I: 5 : True; Imin: -200 : True; Imax: 1000 : True; IWindow: 10 : True 00:00:01.0 D: 0 : True; Dmin: -1000 : True; Dmax: 1000 : True 00:00:01.0 Result min: -6000 : True; Result max: 8000 : True 00:00:01.0 ------------------------------------------------------------------ 00:00:01.0 pump: SetSettings(PumpSettings) 00:00:01.0 pump: GetPumpSideState(A) --> manualMode 00:00:01.0 pump: GetPumpSideState(B) --> manualMode 00:00:01.0 pump: GetPistonPosition(A) --> 217.79719389542 00:00:01.0 pump: GetPistonPosition(B) --> 43.376598086645 00:00:01.0 ------------------------------------------------------- 00:00:01.0 --- Optional Refill of Pump Heads: 00:00:01.0 --- initiated by channel A: False, B: False 00:00:01.0 pump: GetPistonPosition(A) --> 217.79719389542 00:00:01.0 pump: GetPistonPosition(B) --> 43.376598086645 00:00:01.0 --- Piston position channel A: 217.79719389542, B: 43.376598086645 00:00:01.0 ------------------------------------------------------- 00:00:01.0 pump: SetDigitalOutput(PO2, False) 00:00:01.0 pump: GetCurrentPressure(A) --> 7.580369 00:00:01.0 pump: GetCurrentPressure(B) --> 7.931384 00:00:01.0 pump: GetSettings() --> PumpSettings 00:00:01.0 pump: GetSetManualValvePosition(A) --> 240 00:00:01.0 pump: GetSetManualValvePosition(B) --> 240 00:00:01.0 ------------------------------------------------------- 00:00:01.0 --- Reduce pressure --- 00:00:01.0 ------------------------------------------------------- 00:00:01.0 --- Pressure channel A: 7.5803689956665 00:00:01.0 --- Pressure channel B: 7.93138360977173 00:00:01.0 pump: GetSettings() --> PumpSettings 00:00:01.0 ------------------------------------------------------------------ 00:00:01.0 PID settings changed: 00:00:01.0 ------------------------------------------------------------------ 00:00:01.0 P: 250 : True; Pmin: -5500 : True; Pmax: 32000 : True 00:00:01.0 I: 50 : True; Imin: -20000 : True; Imax: 20000 : True; IWindow: 50 : True 00:00:01.0 D: 1500 : True; Dmin: -10000 : True; Dmax: 10000 : True 00:00:01.0 Result min: -32000 : True; Result max: 32000 : True 00:00:01.0 ------------------------------------------------------------------ 00:00:01.1 pump: SetSettings(PumpSettings) 00:00:01.3 --- Set pressure channel A: 10 00:00:01.3 --- Set pressure channel B: 5 00:00:01.3 --- Reduce pressure flow controlled: True 00:00:02.6 -------------------------------------------------------- 00:00:02.6 --- Log PID parameter called from: reducePressure 00:00:02.6 -------------------------------------------------------- 00:00:02.6 --- P: 175; Pmin: -5000; Pmax: 5000 00:00:02.6 --- I: 5; Imin: -200; Imax: 1000; IWindow: 10 00:00:02.6 --- D: 0; Dmin: -1000; Dmax: 1000 00:00:02.6 --- Result min: -6000; Result max: 8000 00:00:02.6 -------------------------------------------------------- 00:00:02.6 -------------------------------------------------------- 00:00:02.6 --- Log instrument settings called from: reducePressure 00:00:02.6 -------------------------------------------------------- 00:00:02.6 --- HomeSpeed: 2000; PurgeSpeed: 2000 00:00:02.6 --- PressureTrackingExcessFactor: 96 00:00:02.6 --- FlowResistanceA: 300; FlowResistanceB: 100; FlowResistanceOut: 150 00:00:02.6 --- FC_FlowFilterDepth: 5; FC_PressureFilterDepth: 0 00:00:02.6 --- PC_OutputFilterDepth: 0 00:00:02.6 --- CompressibilityFactorB: 2.29999995231628; PressurePIDNegativeResultDivisor: 1 00:00:02.6 --- ExternalZeroPressureOffsetA: 130.617385864258; ExternalZeroPressureOffsetB: 127.978851318359 00:00:02.6 --- ExternalPressureScalingFactorA: 0.0197199992835522; ExternalPressureScalingFactorB: 0.0197199992835522 00:00:02.6 -------------------------------------------------------- 00:00:02.6 --- Final pressure channel A: 7.63952922821045 00:00:02.6 --- Final pressure channel B: 0 00:00:02.6 ------------------------------------------------------- 00:00:02.6 pump: GetSetManualValvePosition(A) --> 240 00:00:02.6 pump: GetCurrentPressure(A) --> 7.639529 00:00:02.6 pump: SetManualValvePosition(A, 0, True) 00:00:03.2 pump: GetSetManualValvePosition(B) --> 240 00:00:03.2 pump: GetCurrentPressure(B) --> 0 00:00:03.2 pump: SetManualValvePosition(B, 180, False) 00:00:03.6 pump: GetSettings() --> PumpSettings 00:00:03.6 ------------------------------------------------------------------ 00:00:03.6 PID settings changed: 00:00:03.6 ------------------------------------------------------------------ 00:00:03.6 P: 250 : True; Pmin: -10000 : True; Pmax: 10000 : True 00:00:03.6 I: 20 : True; Imin: -10000 : True; Imax: 20000 : True; IWindow: 10 : True 00:00:03.6 D: 1500 : True; Dmin: -10000 : True; Dmax: 10000 : True 00:00:03.6 Result min: -10000 : True; Result max: 32000 : True 00:00:03.6 ------------------------------------------------------------------ 00:00:03.6 pump: SetSettings(PumpSettings) 00:00:03.6 status: SetStatus(equilibrate separation-column, this finishes at about: 4.85 min., ) 00:00:03.9 autosampler-none: MoveValveDrive(Valve Drive 2, 180 deg) 00:00:03.9 --- MaxPressureLimitA: 800 bar --- 00:00:03.9 pump: GetCurrentPressure(A) --> 7.639529 00:00:03.9 pump: GetCurrentPressure() --> 7.639529 00:00:03.9 pump: GetSettings() --> PumpSettings 00:00:03.9 pump: GetSetManualValvePosition(A) --> 0 00:00:03.9 ------------------------------------------------------- 00:00:03.9 --- Reduce pressure --- 00:00:03.9 ------------------------------------------------------- 00:00:03.9 --- Pressure channel A: 7.63952922821045 00:00:03.9 --- Pressure channel B: 7.63952922821045 00:00:03.9 pump: Manualmode_Pump_constantSpeed(A, 0) 00:00:03.9 pump: GetSetPistonSpeed(A) --> 0 00:00:03.9 pump: Manualmode_Pump_constantSpeed(, 0) 00:00:03.9 pump: GetSetPistonSpeed() --> 0 00:00:03.9 pump: IsIdle() --> True 00:00:03.9 ------------------------------------------------------- 00:00:04.0 pump: SetMaxPressureLimit(A, 820) 00:00:04.0 pump: GetMaxPressureLimit(A) --> 820 00:00:04.0 ------------------------------------------------------- 00:00:04.0 --- Separation Column Equilibration: 00:00:04.0 ------------------------------------------------------- 00:00:04.0 --- ovenTemp [C]: 50 00:00:04.0 --- Separator Unity-Flow @ ovenTemp [ul/min/bar]: 0.00058605984562038 00:00:04.0 --- Separator Volume [ul]: 0.463875790256618 00:00:04.0 --- Separator Equilibration Volume Multiplier: 4 00:00:04.0 --- Separator Equilibration Pressure [bar]: 800 00:00:04.0 --- Separator Equilibration Flow [ul/min]: 0.457694594849779 00:00:04.0 --- Separator Equilibration Volume [ul]: 1.85550316102647 00:00:04.0 --- Separator Equilibration Time [min]: 4.05402026133927 00:00:04.0 --- max. Volume (10*V+2ul/min*t) [ul]: 26.6630721329433 00:00:04.0 --- Separator Equilibration Time Out [min]: 40.5402026133927 00:00:04.0 ------------------------------------------------------- 00:00:04.0 pump: Manualmode_Pump_constantPressure(A, 800) 00:00:04.0 pump: GetPistonPosition(A) --> 217.79740333493 00:00:04.0 --- Injection delay: 237.164855895902s 00:00:29.0 pump: GetPistonPosition(A) --> 300.423803398464 00:00:29.0 pump: GetPistonPosition(A) --> 300.423803398464 00:00:29.0 pump: GetPistonPosition(A) --> 300.423803398464 00:04:01.5 status: SetStatus(aspirate & inject, ) 00:04:02.0 autosampler-left: LeaveObject() 00:04:06.0 autosampler-left: MoveToObject(LCMS Wash 1, 3) 00:04:06.7 autosampler-left: PenetrateObject(LCMS Wash 1, 3) 00:04:06.8 autosampler-left: EmptySyringe() 00:04:07.8 autosampler-left: LeaveObject() 00:04:08.5 Cleaning syringe and needle exterior 00:04:23.9 autosampler-left: CleanSyringe(LCMS Wash 1, 1, 30 mm, 40 uL/s, 500 ms, 30 mm, 40 uL/s, 80 %, 2) 00:04:23.9 autosampler-left: ChangeTool(LCP 1) --> False 00:04:27.3 autosampler-left: MoveToObject(Item Position 3, 1, True, True, True) 00:04:30.5 autosampler-left: PenetrateWithBottomSense(Item Position 3, 1, 1 mm) 00:04:31.5 autosampler-left: AspirateSyringe(2 uL, 5 uL/s, 500 ms) 00:04:32.5 autosampler-left: LeaveObject() 00:04:32.9 pump: GetPistonPosition(A) --> 308.302498894647 00:04:32.9 pump: GetCurrentPressure(A) --> 800.0089 00:04:32.9 --- act. pressure [bar]: 800.008850097656 00:04:32.9 --- used volume [uL]: 7.87869549618279 00:04:32.9 --- average flow (incl. leaks) [uL/min]: 1.93852023838169 00:04:32.9 --- act. equilibration time [min]: 4.06428333333267 00:04:32.9 ------------------------------------------------------- 00:04:32.9 status: RemoveStatus(equilibrate separation-column, this finishes at about: 4.85 min.) 00:04:32.9 status: SetStatus(equilibrate trap-column, this finishes at about: 5.68 min., ) 00:04:32.9 --- MaxPressureLimitA: 217.5 bar --- 00:04:32.9 pump: GetCurrentPressure(A) --> 800.068 00:04:32.9 pump: GetCurrentPressure() --> 800.068 00:04:32.9 pump: GetSettings() --> PumpSettings 00:04:32.9 pump: GetSetManualValvePosition(A) --> 0 00:04:32.9 ------------------------------------------------------- 00:04:32.9 --- Reduce pressure --- 00:04:32.9 ------------------------------------------------------- 00:04:32.9 --- Pressure channel A: 800.067993164063 00:04:32.9 --- Pressure channel B: 800.067993164063 00:04:32.9 pump: GetSettings() --> PumpSettings 00:04:32.9 ------------------------------------------------------------------ 00:04:32.9 PID settings changed: 00:04:32.9 ------------------------------------------------------------------ 00:04:32.9 P: 250 : True; Pmin: -32000 : True; Pmax: 32000 : True 00:04:32.9 I: 20 : True; Imin: -20000 : True; Imax: 20000 : True; IWindow: 100 : True 00:04:32.9 D: 1500 : True; Dmin: -10000 : True; Dmax: 10000 : True 00:04:32.9 Result min: -32000 : True; Result max: 32000 : True 00:04:32.9 ------------------------------------------------------------------ 00:04:32.9 pump: SetSettings(PumpSettings) 00:04:33.0 --- Set pressure channel A: 217.5 00:04:33.0 --- Set pressure channel B: 217.5 00:04:33.0 --- Reduce pressure flow controlled: False 00:04:33.2 autosampler-left: AspirateSyringe(0.5 uL, 5 uL/s, 500 ms) 00:04:43.6 autosampler-left: MoveToObject(Rack 2, 21, True, True, True) 00:04:47.2 autosampler-left: PenetrateWithBottomSense(Rack 2, 21, 1 mm) 00:04:48.5 -------------------------------------------------------- 00:04:48.5 --- Log PID parameter called from: reducePressure 00:04:48.5 -------------------------------------------------------- 00:04:48.5 --- P: 250; Pmin: -10000; Pmax: 10000 00:04:48.5 --- I: 20; Imin: -10000; Imax: 20000; IWindow: 10 00:04:48.5 --- D: 1500; Dmin: -10000; Dmax: 10000 00:04:48.5 --- Result min: -10000; Result max: 32000 00:04:48.5 -------------------------------------------------------- 00:04:48.5 -------------------------------------------------------- 00:04:48.5 --- Log instrument settings called from: reducePressure 00:04:48.5 -------------------------------------------------------- 00:04:48.5 --- HomeSpeed: 2000; PurgeSpeed: 2000 00:04:48.5 --- PressureTrackingExcessFactor: 96 00:04:48.5 --- FlowResistanceA: 300; FlowResistanceB: 100; FlowResistanceOut: 150 00:04:48.5 --- FC_FlowFilterDepth: 5; FC_PressureFilterDepth: 0 00:04:48.5 --- PC_OutputFilterDepth: 0 00:04:48.5 --- CompressibilityFactorB: 2.29999995231628; PressurePIDNegativeResultDivisor: 1 00:04:48.5 --- ExternalZeroPressureOffsetA: 130.617385864258; ExternalZeroPressureOffsetB: 127.978851318359 00:04:48.5 --- ExternalPressureScalingFactorA: 0.0197199992835522; ExternalPressureScalingFactorB: 0.0197199992835522 00:04:48.5 -------------------------------------------------------- 00:04:48.5 --- Final pressure channel A: 216.987045288086 00:04:48.5 --- Final pressure channel B: 216.987045288086 00:04:48.5 ------------------------------------------------------- 00:04:48.6 pump: SetMaxPressureLimit(A, 237.5) 00:04:48.6 pump: GetMaxPressureLimit(A) --> 237.5 00:04:48.8 autosampler-none: MoveValveDrive(Valve Drive 2, 60 deg) 00:04:48.8 ------------------------------------------------------- 00:04:48.8 --- Trap Column Equilibration 00:04:48.8 ------------------------------------------------------- 00:04:48.8 --- Trap Temperature: fixed to 20 C 00:04:48.8 --- Trap Unity-Flow @ 20C [ul/bar]: 2.78838695282287 00:04:48.8 --- Trap Volume [ul]: 0.148440252882118 00:04:48.8 --- Trap Equilibration Volume Multiplier: 10 00:04:48.8 --- Trap Equilibration Pressure [bar]: 217.5 00:04:48.8 --- Trap Equilibration Flow [ul/min]: 9.99557465783688 00:04:48.8 --- Trap Equilibration Volume (+5) [ul]: 6.48440252882118 00:04:48.8 --- Trap Equilibration Time [min]: 0.648727336925764 00:04:48.8 --- max. Volume (10*V+2ul/min*t) [ul]: 66.1414799620633 00:04:48.8 ------------------------------------------------------- 00:04:48.8 pump: Manualmode_Pump_constantPressure(A, 217.5) 00:04:48.8 pump: GetPistonPosition(A) --> 250.320846000973 00:04:48.9 autosampler-left: AspirateSyringe(5 uL, 5 uL/s, 500 ms) 00:04:56.5 autosampler-left: LeaveObject() 00:04:57.7 autosampler-left: AspirateSyringe(1 uL, 5 uL/s, 500 ms) 00:05:01.0 autosampler-left: MoveToObject(LCMS Wash 1, 1, True, True, True) 00:05:01.9 autosampler-left: PenetrateObject(LCMS Wash 1, 1, 30 mm) 00:05:05.8 pump: GetPistonPosition(A) --> 252.08537387474 00:05:05.8 pump: GetPistonPosition(A) --> 252.08537387474 00:05:05.8 pump: GetPistonPosition(A) --> 252.08537387474 00:05:07.0 autosampler-left: Wait(5 s) 00:05:07.8 autosampler-left: LeaveObject() 00:05:10.8 autosampler-left: MoveToObject(LCInjector1) 00:05:11.6 autosampler-left: DispenseSyringe(0.75 uL, 1 uL/s) 00:05:14.1 autosampler-left: PenetrateWithConstForce(LCInjector1) 00:05:14.2 autosampler-left: MoveValveDrive(Valve Drive 1, 0 deg) 00:05:15.6 autosampler-left: Wait(1000 ms) 00:05:22.4 autosampler-left: DispenseSyringe(6.75 uL, 1 uL/s) 00:05:23.4 autosampler-left: Wait(1000 ms) 00:05:24.1 status: RemoveStatus(aspirate & inject) 00:05:45.1 pump: GetPistonPosition(A) --> 256.027653775974 00:05:45.1 pump: GetPistonPosition(A) --> 256.027653775974 00:06:11.1 pump: GetPistonPosition(A) --> 258.64062110572 00:06:11.1 pump: GetCurrentPressure(A) --> 217.4603 00:06:11.1 ------------------------------------------------------- 00:06:11.1 --- Finished: Trap Column Equilibration (const_pressure_load_and_equilibration) 00:06:11.1 ------------------------------------------------------- 00:06:11.1 --- act. pressure [bar]: 217.460327148438 00:06:11.1 --- used volume [uL]: 6.55524723098048 00:06:11.1 --- average flow [uL/min]: 6.02587418391676 00:06:11.1 --- act. equilibration time [min]: 1.0878500000011 00:06:11.1 ------------------------------------------------------- 00:06:11.1 status: RemoveStatus(equilibrate trap-column, this finishes at about: 5.68 min.) 00:06:13.1 --- MaxPressureLimitA: 217.5 bar --- 00:06:13.1 pump: GetCurrentPressure(A) --> 217.3617 00:06:13.1 pump: GetCurrentPressure() --> 217.3617 00:06:13.1 pump: GetSettings() --> PumpSettings 00:06:13.1 pump: GetSetManualValvePosition(A) --> 0 00:06:13.1 ------------------------------------------------------- 00:06:13.1 --- Reduce pressure --- 00:06:13.1 ------------------------------------------------------- 00:06:13.1 --- Pressure channel A: 217.361724853516 00:06:13.1 --- Pressure channel B: 217.361724853516 00:06:13.1 pump: Manualmode_Pump_constantSpeed(A, 0) 00:06:13.1 pump: GetSetPistonSpeed(A) --> 0 00:06:13.1 pump: Manualmode_Pump_constantSpeed(, 0) 00:06:13.1 pump: GetSetPistonSpeed() --> 0 00:06:13.1 pump: IsIdle() --> False 00:06:13.1 ------------------------------------------------------- 00:06:13.2 pump: SetMaxPressureLimit(A, 237.5) 00:06:13.2 pump: GetMaxPressureLimit(A) --> 237.5 00:06:13.2 status: SetStatus(load trap-column, this finishes at about: 8.49 min., ) 00:06:13.4 autosampler-left: MoveValveDrive(Valve Drive 1, 60 deg) 00:06:14.5 ------------------------------------------------------- 00:06:14.5 --- Sample Loading on Trap Column 00:06:14.5 ------------------------------------------------------- 00:06:14.5 --- Trap Temperature: fixed to 20 C 00:06:14.5 --- Trap Unity-Flow [ul/bar]: 2.78838695282287 00:06:14.5 --- Trap Volume [ul]: 0.148440252882118 00:06:14.5 --- Sample Volume [ul]: 5 00:06:14.5 --- Sample Volume Multiplier: 4 00:06:14.5 --- Trap Loading Pressure [bar]: 217.5 00:06:14.5 --- Trap Loading Flow [uL/min]: 9.99557465783688 00:06:14.5 --- Trap Loading Volume (+2) [uL]: 22 00:06:14.5 --- Trap Loading Time [min]: 2.200974006307 00:06:14.5 --- max. Volume (10*V+2ul/min*t) [ul]: 224.401948012614 00:06:14.5 ------------------------------------------------------- 00:06:14.5 pump: Manualmode_Pump_constantPressure(A, 217.5) 00:06:14.5 pump: GetPistonPosition(A) --> 258.848594539388 00:06:14.6 autosampler-left: EmptySyringe(5 uL/s) 00:06:25.8 pump: GetPistonPosition(A) --> 260.770202045833 00:06:25.8 pump: GetPistonPosition(A) --> 260.770202045833 00:06:25.8 pump: GetPistonPosition(A) --> 260.770202045833 00:07:13.4 autosampler-left: CleanInjector(LCInjector1, LCP 1, 200 uL, 0 mm, 5 uL/s, 5 s, 20 mm, 40 uL/s, 1000 ms, 2, False) 00:08:14.4 autosampler-left: CleanInjector(LCInjector1, LCP 1, 200 uL, 0 mm, 5 uL/s, 5 s, 20 mm, 40 uL/s, 1000 ms, 1, True) 00:08:30.1 autosampler-left: CleanSyringe(LCMS Wash 1, 2, 30 mm, 40 uL/s, 500 ms, 30 mm, 40 uL/s, 80 %, 2) 00:08:37.9 pump: GetPistonPosition(A) --> 274.099141916974 00:08:37.9 pump: GetPistonPosition(A) --> 274.099141916974 00:08:50.3 autosampler-left: CleanSyringe(LCMS Wash 1, 1, 30 mm, 40 uL/s, 500 ms, 30 mm, 40 uL/s, 80 %, 3) 00:08:53.6 autosampler-left: MoveToHome() 00:10:03.4 pump: GetPistonPosition(A) --> 282.78627392268 00:10:03.4 pump: GetCurrentPressure(A) --> 217.5589 00:10:03.4 ------------------------------------------------------- 00:10:03.4 --- Finished: Sample Loading on Trap Column (const_pressure_load_and_equilibration) 00:10:03.4 ------------------------------------------------------- 00:10:03.4 --- act. pressure [bar]: 217.55891418457 00:10:03.4 --- used volume [uL]: 22.016071876847 00:10:03.4 --- average flow [uL/min]: 6.06899040058807 00:10:03.4 --- act. loading time [min]: 3.62763333333229 00:10:03.4 ------------------------------------------------------- 00:10:03.4 --- Separator: Name: IonOpticks 25 cm, Func: Separator, ColDia: 0.075 mm, Len: 250 mm, PartDia: 1.6 µm, Porosity: 42 % --- 00:10:03.4 --- Trap: Name: Thermo Trap Cartridge 5mm, Func: Separator, ColDia: 0.3 mm, Len: 5 mm, PartDia: 5 µm, Porosity: 42 % --- 00:10:03.4 --- MaxPressureLimitAB: 1000 bar --- 00:10:03.4 pump: GetCurrentPressure(A) --> 217.5589 00:10:03.4 pump: GetCurrentPressure(B) --> 0.003944032 00:10:03.4 pump: GetSettings() --> PumpSettings 00:10:03.4 pump: GetSetManualValvePosition(A) --> 0 00:10:03.4 ------------------------------------------------------- 00:10:03.4 --- Reduce pressure --- 00:10:03.4 ------------------------------------------------------- 00:10:03.4 --- Pressure channel A: 217.55891418457 00:10:03.4 --- Pressure channel B: 0.003944031894207 00:10:03.4 pump: Manualmode_Pump_constantSpeed(A, 0) 00:10:03.4 pump: GetSetPistonSpeed(A) --> 0 00:10:03.4 pump: Manualmode_Pump_constantSpeed(B, 0) 00:10:03.4 pump: GetSetPistonSpeed(B) --> 0 00:10:03.4 pump: IsIdle() --> False 00:10:03.4 ------------------------------------------------------- 00:10:03.5 pump: SetMaxPressureLimit(A, 1020) 00:10:03.5 pump: GetMaxPressureLimit(A) --> 1020 00:10:03.6 pump: SetMaxPressureLimit(B, 1020) 00:10:03.6 pump: GetMaxPressureLimit(B) --> 1020 00:10:03.6 status: RemoveStatus(load trap-column, this finishes at about: 8.49 min.) 00:10:03.6 pump: GetSettings() --> PumpSettings 00:10:03.6 ------------------------------------------------------------------ 00:10:03.6 PID settings changed: 00:10:03.6 ------------------------------------------------------------------ 00:10:03.6 P: 100 : True; Pmin: -10000 : True; Pmax: 10000 : True 00:10:03.6 I: 100 : True; Imin: -1000 : True; Imax: 1000 : True; IWindow: 100 : True 00:10:03.6 D: 5000 : True; Dmin: -32000 : True; Dmax: 32000 : True 00:10:03.6 Result min: -5000 : True; Result max: 25000 : True 00:10:03.6 ------------------------------------------------------------------ 00:10:03.6 pump: SetSettings(PumpSettings) 00:10:03.6 pump: GetSettings() --> PumpSettings 00:10:03.6 ------------------------------------------------------------------ 00:10:03.6 PID settings changed: 00:10:03.6 ------------------------------------------------------------------ 00:10:03.6 P: 100 : False; Pmin: -10000 : False; Pmax: 10000 : False 00:10:03.6 I: 100 : False; Imin: -1000 : False; Imax: 1000 : False; IWindow: 2 : True 00:10:03.6 D: 5000 : False; Dmin: -32000 : False; Dmax: 32000 : False 00:10:03.6 Result min: -5000 : False; Result max: 25000 : False 00:10:03.6 ------------------------------------------------------------------ 00:10:03.6 pump: SetSettings(PumpSettings) 00:10:03.6 status: SetStatus(preparing gradient start, ) 00:10:04.0 --- Starting conditioning paths --- 00:10:04.0 pump: GetSettings() --> PumpSettings 00:10:04.0 ------------------------------------------------------------------ 00:10:04.0 PID settings changed: 00:10:04.0 ------------------------------------------------------------------ 00:10:04.0 P: 100 : True; Pmin: -10000 : True; Pmax: 10000 : True 00:10:04.0 I: 100 : True; Imin: -1000 : True; Imax: 1000 : True; IWindow: 100 : True 00:10:04.0 D: 5000 : True; Dmin: -32000 : True; Dmax: 32000 : True 00:10:04.0 Result min: -5000 : True; Result max: 25000 : True 00:10:04.0 ------------------------------------------------------------------ 00:10:04.0 pump: SetSettings(PumpSettings) 00:10:04.1 pump: Manualmode_Pump_constantPressure(A, 0) 00:10:04.1 pump: GetTargetPressure(A) --> 0 00:10:04.1 pump: GetCurrentPressure(A) --> 217.204 00:10:05.1 pump: GetCurrentPressure(A) --> 202.4337 00:10:06.1 pump: GetCurrentPressure(A) --> 182.9503 00:10:07.1 pump: GetCurrentPressure(A) --> 163.6444 00:10:08.1 pump: GetCurrentPressure(A) --> 144.1808 00:10:09.1 pump: GetCurrentPressure(A) --> 124.2636 00:10:10.1 pump: GetCurrentPressure(A) --> 104.8197 00:10:11.1 pump: GetCurrentPressure(A) --> 85.94765 00:10:12.1 pump: GetCurrentPressure(A) --> 66.93757 00:10:13.1 pump: GetCurrentPressure(A) --> 50.53053 00:10:14.1 pump: GetCurrentPressure(A) --> 33.17693 00:10:15.1 pump: GetCurrentPressure(A) --> 20.63501 00:10:16.1 pump: GetCurrentPressure(A) --> 12.96393 00:10:16.2 autosampler-none: MoveValveDrive(Valve Drive 2, 0 deg) 00:10:16.2 pump: SetManualValvePosition(A, 240, False) 00:10:17.0 pump: SetManualValvePosition(B, 240, False) 00:10:19.0 --- Set constant binary flow --- 00:10:19.0 pump: Manualmode_Pump_constantFlow_binary(1, 1) 00:10:19.0 pump: GetSetFlow(A) --> 1 00:10:19.0 pump: GetSetFlow(B) --> 1 00:10:19.0 00:10:19.0 --- Starting spline flow reduction B/A --- 00:10:19.1 pump: GetSetExternalTemperature() --> 50 00:10:19.1 --- Oven temperatur: 50 00:10:19.1 --- Separator: Name: IonOpticks 25 cm, Func: Separator, ColDia: 0.075 mm, Len: 250 mm, PartDia: 1.6 µm, Porosity: 42 % 00:10:19.1 --- Spline fitting time: 97.6577595902957 s 00:10:19.1 --- Spline fitting time adapted: 10 s 00:10:19.1 --- y = 0x + 1 00:10:19.1 --- p0.Time -1, p0.Flow 1 00:10:19.1 --- p1.Time 0, p1.Flow 1 00:10:19.1 --- p2.Time 9, p2.Flow 1 00:10:19.1 --- p3.Time 10, p3.Flow 1, p3.Mix 0 00:10:19.1 --- p4.Time 40, p4.Flow 1, p4.Mix 0 00:10:19.1 --- Strategy function end 00:10:19.1 --- y = 0x + 0 00:10:19.1 --- p0.Time -1, p0.Flow 1 00:10:19.1 --- p1.Time 0, p1.Flow 1 00:10:19.1 --- p2.Time 9, p2.Flow 0 00:10:19.1 --- p3.Time 10, p3.Flow 0, p3.Mix 0 00:10:19.1 --- p4.Time 40, p4.Flow 0, p4.Mix 0 00:10:19.1 --- Strategy function end 00:10:19.1 xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx 00:10:19.1 XXXXX Time 0, Flow 2, Mix 50 00:10:19.1 XXXXX Time 1, Flow 1.94545, Mix 48.59813 00:10:19.2 XXXXX Time 2, Flow 1.84848, Mix 45.90163 00:10:19.2 XXXXX Time 3, Flow 1.72121, Mix 41.9014 00:10:19.2 XXXXX Time 4, Flow 1.57575, Mix 36.53846 00:10:19.2 XXXXX Time 5, Flow 1.42424, Mix 29.78723 00:10:19.2 XXXXX Time 6, Flow 1.27878, Mix 21.80094 00:10:19.2 XXXXX Time 7, Flow 1.15151, Mix 13.15789 00:10:19.3 XXXXX Time 8, Flow 1.05454, Mix 5.17241 00:10:19.3 XXXXX Time 9, Flow 1, Mix 0 00:10:19.3 --- Gradient is created, switching valves A and B 00:10:19.3 pump: GetSetManualValvePosition(A) --> 240 00:10:19.3 pump: GetCurrentPressure(A) --> 4.247689 00:10:19.3 pump: SetManualValvePosition(A, 240, False) 00:10:19.5 pump: GetSetManualValvePosition(B) --> 240 00:10:19.5 pump: GetCurrentPressure(B) --> 0.358904 00:10:19.5 pump: SetManualValvePosition(B, 240, False) 00:10:19.7 --- Valves A and B switched to MixTee, starting gradient 00:10:19.7 --- LoadGradient() --- 00:10:19.7 --- Loading gradient method index: 0 00:10:19.7 pump: LoadMethod(0, ZrData.ZrPumpMethod) 00:10:20.2 pump: GetMethodState(0) --> loaded 00:10:20.2 --- Set gradient start 00:10:20.2 --- StartGradient() --- 00:10:20.2 --- Starting gradient method index: 0 00:10:20.2 pump: SetMethodSignal(0, StartTrunk) 00:10:20.4 pump: GetMethodState(0) --> runningTrunk 00:10:20.4 --- Start GradientSplineFit 00:10:20.4 pump: GetMethodState(0) --> runningTrunk 00:10:21.4 pump: GetCurrentPressure(A) --> 74.15509 00:10:21.4 pump: GetCurrentPressure(B) --> 62.1811 00:10:21.4 --- GradientSplineFit is running 00:10:29.4 --- GradientSplineFit has finished 00:10:29.4 --- End GradientSplineFit 00:10:29.4 --- Finished spline flow reduction B --- 00:10:30.4 pump: GetSetExternalTemperature() --> 50 00:10:30.4 --- Oven temperatur: 50 00:10:30.4 --- Separator: Name: IonOpticks 25 cm, Func: Separator, ColDia: 0.075 mm, Len: 250 mm, PartDia: 1.6 µm, Porosity: 42 % 00:10:30.4 --- Spline fitting time: 97.6577595902957 s 00:10:30.4 --- Spline fitting time adapted: 60 s 00:10:30.4 --- y = 0x + 0.01 00:10:30.4 --- p0.Time -1, p0.Flow 1 00:10:30.4 --- p1.Time 0, p1.Flow 1 00:10:30.4 --- p2.Time 59, p2.Flow 0.01 00:10:30.4 --- p3.Time 60, p3.Flow 0.01, p3.Mix 0 00:10:30.4 --- p4.Time 90, p4.Flow 0.01, p4.Mix 0 00:10:30.4 --- Strategy function end 00:10:30.4 --- y = 0x + 0 00:10:30.4 --- p0.Time -1, p0.Flow 0 00:10:30.4 --- p1.Time 0, p1.Flow 0 00:10:30.4 --- p2.Time 59, p2.Flow 0 00:10:30.4 --- p3.Time 60, p3.Flow 0, p3.Mix 0 00:10:30.4 --- p4.Time 90, p4.Flow 0, p4.Mix 0 00:10:30.4 --- Strategy function end 00:10:30.4 xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx 00:10:30.4 XXXXX Time 0, Flow 1, Mix 0 00:10:30.5 XXXXX Time 1, Flow 0.99837, Mix 0 00:10:30.5 XXXXX Time 2, Flow 0.99518, Mix 0 00:10:30.5 XXXXX Time 3, Flow 0.99048, Mix 0 00:10:30.5 XXXXX Time 4, Flow 0.98432, Mix 0 00:10:30.5 XXXXX Time 5, Flow 0.97675, Mix 0 00:10:30.6 XXXXX Time 6, Flow 0.96784, Mix 0 00:10:30.6 XXXXX Time 7, Flow 0.95763, Mix 0 00:10:30.6 XXXXX Time 8, Flow 0.94619, Mix 0 00:10:30.6 XXXXX Time 9, Flow 0.93356, Mix 0 00:10:30.6 XXXXX Time 10, Flow 0.91981, Mix 0 00:10:30.6 XXXXX Time 11, Flow 0.90498, Mix 0 00:10:30.6 XXXXX Time 12, Flow 0.88914, Mix 0 00:10:30.7 XXXXX Time 13, Flow 0.87233, Mix 0 00:10:30.7 XXXXX Time 14, Flow 0.85462, Mix 0 00:10:30.7 XXXXX Time 15, Flow 0.83605, Mix 0 00:10:30.7 XXXXX Time 16, Flow 0.81668, Mix 0 00:10:30.7 XXXXX Time 17, Flow 0.79658, Mix 0 00:10:30.7 XXXXX Time 18, Flow 0.77578, Mix 0 00:10:30.7 XXXXX Time 19, Flow 0.75435, Mix 0 00:10:30.8 XXXXX Time 20, Flow 0.73235, Mix 0 00:10:30.8 XXXXX Time 21, Flow 0.70982, Mix 0 00:10:30.8 XXXXX Time 22, Flow 0.68682, Mix 0 00:10:30.8 XXXXX Time 23, Flow 0.66341, Mix 0 00:10:30.8 XXXXX Time 24, Flow 0.63964, Mix 0 00:10:30.8 XXXXX Time 25, Flow 0.61558, Mix 0 00:10:30.8 XXXXX Time 26, Flow 0.59126, Mix 0 00:10:30.9 XXXXX Time 27, Flow 0.56675, Mix 0 00:10:30.9 XXXXX Time 28, Flow 0.5421, Mix 0 00:10:30.9 XXXXX Time 29, Flow 0.51737, Mix 0 00:10:30.9 XXXXX Time 30, Flow 0.49262, Mix 0 00:10:30.9 XXXXX Time 31, Flow 0.46789, Mix 0 00:10:30.9 XXXXX Time 32, Flow 0.44324, Mix 0 00:10:30.9 XXXXX Time 33, Flow 0.41873, Mix 0 00:10:31.0 XXXXX Time 34, Flow 0.39441, Mix 0 00:10:31.0 XXXXX Time 35, Flow 0.37035, Mix 0 00:10:31.0 XXXXX Time 36, Flow 0.34658, Mix 0 00:10:31.0 XXXXX Time 37, Flow 0.32317, Mix 0 00:10:31.0 XXXXX Time 38, Flow 0.30017, Mix 0 00:10:31.0 XXXXX Time 39, Flow 0.27764, Mix 0 00:10:31.0 XXXXX Time 40, Flow 0.25564, Mix 0 00:10:31.0 XXXXX Time 41, Flow 0.23421, Mix 0 00:10:31.1 XXXXX Time 42, Flow 0.21341, Mix 0 00:10:31.1 XXXXX Time 43, Flow 0.19331, Mix 0 00:10:31.1 XXXXX Time 44, Flow 0.17394, Mix 0 00:10:31.1 XXXXX Time 45, Flow 0.15537, Mix 0 00:10:31.1 XXXXX Time 46, Flow 0.13766, Mix 0 00:10:31.1 XXXXX Time 47, Flow 0.12085, Mix 0 00:10:31.1 XXXXX Time 48, Flow 0.10501, Mix 0 00:10:31.2 XXXXX Time 49, Flow 0.09018, Mix 0 00:10:31.2 XXXXX Time 50, Flow 0.07643, Mix 0 00:10:31.2 XXXXX Time 51, Flow 0.0638, Mix 0 00:10:31.2 XXXXX Time 52, Flow 0.05236, Mix 0 00:10:31.2 XXXXX Time 53, Flow 0.04215, Mix 0 00:10:31.2 XXXXX Time 54, Flow 0.03324, Mix 0 00:10:31.2 XXXXX Time 55, Flow 0.02567, Mix 0 00:10:31.3 XXXXX Time 56, Flow 0.01951, Mix 0 00:10:31.3 XXXXX Time 57, Flow 0.01481, Mix 0 00:10:31.3 XXXXX Time 58, Flow 0.01162, Mix 0 00:10:31.3 XXXXX Time 59, Flow 0.00999, Mix 0 00:10:31.3 --- Gradient is created, switching valves A and B 00:10:31.3 pump: GetSetManualValvePosition(A) --> 240 00:10:31.3 pump: GetCurrentPressure(A) --> 89.67473 00:10:31.3 pump: SetManualValvePosition(A, 240, False) 00:10:31.5 pump: GetSetManualValvePosition(B) --> 240 00:10:31.5 pump: GetCurrentPressure(B) --> 12.80222 00:10:31.5 pump: SetManualValvePosition(B, 240, False) 00:10:31.7 --- Valves A and B switched to MixTee, starting gradient 00:10:31.7 --- LoadGradient() --- 00:10:31.7 --- Loading gradient method index: 0 00:10:31.7 pump: LoadMethod(0, ZrData.ZrPumpMethod) 00:10:32.2 pump: GetMethodState(0) --> loaded 00:10:32.2 --- Set gradient start 00:10:32.2 --- StartGradient() --- 00:10:32.2 --- Starting gradient method index: 0 00:10:32.2 pump: SetMethodSignal(0, StartTrunk) 00:10:32.4 pump: GetMethodState(0) --> runningTrunk 00:10:32.4 --- Start GradientSplineFit 00:10:32.4 pump: GetMethodState(0) --> runningTrunk 00:10:33.4 pump: GetCurrentPressure(A) --> 90.22688 00:10:33.4 pump: GetCurrentPressure(B) --> 13.82766 00:10:33.4 --- GradientSplineFit is running 00:11:31.4 --- GradientSplineFit has finished 00:11:31.4 --- End GradientSplineFit 00:11:31.4 --- Finished spline flow reduction A --- 00:11:31.4 --- Finished conditioning paths --- 00:11:36.7 autosampler-none: MoveValveDrive(Valve Drive 2, 240 deg) 00:11:36.8 pump: GetSetExternalTemperature() --> 50 00:11:36.8 --- Oven temperatur: 50 00:11:36.8 --- Separator: Name: IonOpticks 25 cm, Func: Separator, ColDia: 0.075 mm, Len: 250 mm, PartDia: 1.6 µm, Porosity: 42 % 00:11:36.8 --- Spline fitting time: 97.6577595902957 s 00:11:36.8 --- Spline fitting time adapted: 53.7117677746626 s 00:11:36.8 --- Press: 541.893115083548; colPress: 511.893115083548, visco: 0.00566684100509561, flow > 0.3 00:11:36.8 --- Flow: 0.3; Mix: 0, calcI: 220.674819441516 00:11:36.8 --- Ki calc: 220.674819441516 00:11:36.8 pump: GetSettings() --> PumpSettings 00:11:36.8 ------------------------------------------------------------------ 00:11:36.8 PID settings changed: 00:11:36.8 ------------------------------------------------------------------ 00:11:36.8 P: 200 : True; Pmin: -32000 : True; Pmax: 32000 : True 00:11:36.8 I: 600 : True; Imin: -32000 : True; Imax: 32000 : True; IWindow: 100 : True 00:11:36.8 D: 1000 : True; Dmin: -32000 : True; Dmax: 32000 : True 00:11:36.8 Result min: -32000 : True; Result max: 32000 : True 00:11:36.8 ------------------------------------------------------------------ 00:11:36.8 pump: SetSettings(PumpSettings) 00:11:36.8 pump: GetSettings() --> PumpSettings 00:11:36.8 ------------------------------------------------------------------ 00:11:36.8 PID settings changed: 00:11:36.8 ------------------------------------------------------------------ 00:11:36.8 P: 200 : False; Pmin: -32000 : False; Pmax: 32000 : False 00:11:36.8 I: 220 : True; Imin: -32000 : False; Imax: 32000 : False; IWindow: 100 : False 00:11:36.8 D: 1000 : False; Dmin: -32000 : False; Dmax: 32000 : False 00:11:36.8 Result min: -32000 : False; Result max: 32000 : False 00:11:36.8 ------------------------------------------------------------------ 00:11:36.8 pump: SetSettings(PumpSettings) 00:11:36.8 --- y = -5.33333333333333E-05x + 0.302864627614649 00:11:36.8 --- p0.Time -1, p0.Flow 0.01 00:11:36.8 --- p1.Time 0, p1.Flow 0.01 00:11:36.8 --- p2.Time 52.7117677746626, p2.Flow 0.300053333333333 00:11:36.8 --- p3.Time 53.7117677746626, p3.Flow 0.3, p3.Mix 0 00:11:36.8 --- p4.Time 1853.71176777466, p4.Flow 0.204, p4.Mix 32 00:11:36.8 --- Strategy function end 00:11:36.8 --- y = 5.33333333333333E-05x + -0.00286462761464867 00:11:36.8 --- p0.Time -1, p0.Flow 0 00:11:36.8 --- p1.Time 0, p1.Flow 0 00:11:36.8 --- p2.Time 52.7117677746626, p2.Flow -5.33333333333336E-05 00:11:36.8 --- p3.Time 53.7117677746626, p3.Flow 0, p3.Mix 0 00:11:36.8 --- p4.Time 1853.71176777466, p4.Flow 0.096, p4.Mix 32 00:11:36.8 --- Strategy function end 00:11:36.8 xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx 00:11:36.8 XXXXX Time 0, Flow 0.01, Mix 0 00:11:36.8 XXXXX Time 1, Flow 0.01059, Mix 0 00:11:36.8 XXXXX Time 2, Flow 0.01175, Mix 0 00:11:36.9 XXXXX Time 3, Flow 0.01347, Mix 0 00:11:36.9 XXXXX Time 4, Flow 0.01571, Mix 0 00:11:36.9 XXXXX Time 5, Flow 0.01845, Mix 0 00:11:36.9 XXXXX Time 6, Flow 0.02168, Mix 0 00:11:36.9 XXXXX Time 7, Flow 0.02537, Mix 0 00:11:36.9 XXXXX Time 8, Flow 0.02949, Mix 0 00:11:36.9 XXXXX Time 9, Flow 0.03402, Mix 0 00:11:36.9 XXXXX Time 10, Flow 0.03894, Mix 0 00:11:37.0 XXXXX Time 11, Flow 0.04424, Mix 0 00:11:37.0 XXXXX Time 12, Flow 0.04988, Mix 0 00:11:37.0 XXXXX Time 13, Flow 0.05584, Mix 0 00:11:37.0 XXXXX Time 14, Flow 0.0621, Mix 0 00:11:37.0 XXXXX Time 15, Flow 0.06864, Mix 0 00:11:37.0 XXXXX Time 16, Flow 0.07544, Mix 0 00:11:37.1 XXXXX Time 17, Flow 0.08247, Mix 0 00:11:37.1 XXXXX Time 18, Flow 0.08971, Mix 0 00:11:37.1 XXXXX Time 19, Flow 0.09713, Mix 0 00:11:37.1 XXXXX Time 20, Flow 0.10472, Mix 0 00:11:37.1 XXXXX Time 21, Flow 0.11246, Mix 0 00:11:37.1 XXXXX Time 22, Flow 0.12031, Mix 0 00:11:37.1 XXXXX Time 23, Flow 0.12826, Mix 0 00:11:37.1 XXXXX Time 24, Flow 0.13629, Mix 0 00:11:37.2 XXXXX Time 25, Flow 0.14437, Mix 0 00:11:37.2 XXXXX Time 26, Flow 0.15248, Mix 0 00:11:37.2 XXXXX Time 27, Flow 0.16059, Mix 0 00:11:37.2 XXXXX Time 28, Flow 0.16869, Mix 0 00:11:37.2 XXXXX Time 29, Flow 0.17675, Mix 0 00:11:37.2 XXXXX Time 30, Flow 0.18476, Mix 0 00:11:37.2 XXXXX Time 31, Flow 0.19267, Mix 0 00:11:37.3 XXXXX Time 32, Flow 0.20049, Mix 0 00:11:37.3 XXXXX Time 33, Flow 0.20817, Mix 0 00:11:37.3 XXXXX Time 34, Flow 0.2157, Mix 0 00:11:37.3 XXXXX Time 35, Flow 0.22306, Mix 0 00:11:37.3 XXXXX Time 36, Flow 0.23022, Mix 0 00:11:37.3 XXXXX Time 37, Flow 0.23717, Mix 0 00:11:37.3 XXXXX Time 38, Flow 0.24387, Mix 0 00:11:37.3 XXXXX Time 39, Flow 0.25031, Mix 0 00:11:37.4 XXXXX Time 40, Flow 0.25646, Mix 0 00:11:37.4 XXXXX Time 41, Flow 0.2623, Mix 0 00:11:37.4 XXXXX Time 42, Flow 0.26781, Mix 0 00:11:37.4 XXXXX Time 43, Flow 0.27297, Mix 0 00:11:37.4 XXXXX Time 44, Flow 0.27775, Mix 0 00:11:37.4 XXXXX Time 45, Flow 0.28213, Mix 0 00:11:37.4 XXXXX Time 46, Flow 0.28609, Mix 0 00:11:37.5 XXXXX Time 47, Flow 0.28961, Mix 0 00:11:37.5 XXXXX Time 48, Flow 0.29265, Mix 0 00:11:37.5 XXXXX Time 49, Flow 0.29521, Mix 0 00:11:37.5 XXXXX Time 50, Flow 0.29726, Mix 0 00:11:37.5 XXXXX Time 51, Flow 0.29877, Mix 0 00:11:37.5 XXXXX Time 52, Flow 0.29972, Mix 0 00:11:37.5 XXXXX Time 53, Flow 0.3001, Mix 0 00:11:37.5 --- Gradient is created, switching valves A and B 00:11:37.5 pump: GetSetManualValvePosition(A) --> 240 00:11:37.5 pump: GetCurrentPressure(A) --> 1.013609 00:11:37.5 pump: SetManualValvePosition(A, 240, False) 00:11:37.7 pump: GetSetManualValvePosition(B) --> 240 00:11:37.7 pump: GetCurrentPressure(B) --> 0 00:11:37.7 pump: SetManualValvePosition(B, 240, False) 00:11:37.9 --- Valves A and B switched to MixTee, starting gradient 00:11:37.9 --- LoadGradient() --- 00:11:37.9 --- Loading gradient method index: 0 00:11:37.9 pump: LoadMethod(0, ZrData.ZrPumpMethod) 00:11:38.4 pump: GetMethodState(0) --> loaded 00:11:38.4 --- Set gradient start 00:11:38.4 --- StartGradient() --- 00:11:38.4 --- Starting gradient method index: 0 00:11:38.4 pump: SetMethodSignal(0, StartTrunk) 00:11:38.6 pump: GetMethodState(0) --> runningTrunk 00:11:38.6 --- Start GradientSplineFit 00:11:38.6 pump: GetMethodState(0) --> runningTrunk 00:11:39.6 pump: GetCurrentPressure(A) --> 0.1853694 00:11:39.6 pump: GetCurrentPressure(B) --> 0 00:11:39.6 --- GradientSplineFit is running 00:12:31.7 --- GradientSplineFit has finished 00:12:31.7 --- End GradientSplineFit 00:12:31.7 status: RemoveStatus(preparing gradient start) 00:12:31.8 Setting signal "lc-ready" 00:12:31.8 Waiting for signal "ms-start"... 00:12:31.8 --- LoadGradient() --- 00:12:31.8 --- Loading gradient method index: 0 00:12:31.8 pump: LoadMethod(0, ZrData.ZrPumpMethod) 00:12:32.3 pump: GetMethodState(0) --> loaded 00:12:32.3 --- Set gradient start 00:12:32.3 --- StartGradient() --- 00:12:32.3 --- Starting gradient method index: 0 00:12:32.3 pump: SetMethodSignal(0, StartTrunk) 00:12:32.5 pump: GetMethodState(0) --> runningTrunk 00:12:32.6 autosampler-none: SetSignal(Output Signal 1) 00:12:32.6 status: SetStatus(wash, ) 00:12:37.4 status: SetStatus(elution, ) 00:12:39.0 autosampler-left: MoveToObject(LCInjector1) 00:12:41.7 autosampler-left: PenetrateWithConstForce(LCInjector1) 00:12:41.8 autosampler-left: MoveValveDrive(Valve Drive 1, 0 deg) 00:13:11.5 autosampler-left: CleanInjector(LCInjector1, LCP 1, 100 uL, 0 mm, 5 uL/s, 5 s, 20 mm, 40 uL/s, 1000 ms, 2, False) 00:14:41.5 autosampler-left: CleanInjector(LCInjector1, LCP 1, 300 uL, 0 mm, 5 uL/s, 5 s, 20 mm, 40 uL/s, 1000 ms, 1, True) 00:14:41.6 autosampler-left: MoveValveDrive(Valve Drive 1, 60 deg) 00:14:44.4 autosampler-left: MoveToHome() 01:12:33.2 Setting signal "lc-done" 01:12:33.2 status: RemoveStatus(elution) 01:12:33.2 status: RemoveStatus(wash) 01:12:33.2 pump: GetCurrentPressure(A) --> 234.2223 01:12:33.2 pump: GetCurrentPressure(B) --> 242.3036 01:12:33.2 pump: GetSettings() --> PumpSettings 01:12:33.2 pump: GetSetManualValvePosition(A) --> 240 01:12:33.2 pump: GetSetManualValvePosition(B) --> 240 01:12:33.2 ------------------------------------------------------- 01:12:33.2 --- Reduce pressure --- 01:12:33.2 ------------------------------------------------------- 01:12:33.2 --- Pressure channel A: 234.222320556641 01:12:33.2 --- Pressure channel B: 242.303573608398 01:12:33.2 pump: GetSettings() --> PumpSettings 01:12:33.2 ------------------------------------------------------------------ 01:12:33.2 PID settings changed: 01:12:33.2 ------------------------------------------------------------------ 01:12:33.2 P: 250 : True; Pmin: -5500 : True; Pmax: 32000 : True 01:12:33.2 I: 50 : True; Imin: -20000 : True; Imax: 20000 : True; IWindow: 50 : True 01:12:33.2 D: 1500 : True; Dmin: -10000 : True; Dmax: 10000 : True 01:12:33.2 Result min: -32000 : True; Result max: 32000 : True 01:12:33.2 ------------------------------------------------------------------ 01:12:33.2 pump: SetSettings(PumpSettings) 01:12:33.4 --- Set pressure channel A: 10 01:12:33.4 --- Set pressure channel B: 5 01:12:33.4 --- Reduce pressure flow controlled: True 01:13:04.5 -------------------------------------------------------- 01:13:04.5 --- Log PID parameter called from: reducePressure 01:13:04.5 -------------------------------------------------------- 01:13:04.5 --- P: 200; Pmin: -32000; Pmax: 32000 01:13:04.5 --- I: 220; Imin: -32000; Imax: 32000; IWindow: 100 01:13:04.5 --- D: 1000; Dmin: -32000; Dmax: 32000 01:13:04.5 --- Result min: -32000; Result max: 32000 01:13:04.5 -------------------------------------------------------- 01:13:04.5 -------------------------------------------------------- 01:13:04.5 --- Log instrument settings called from: reducePressure 01:13:04.5 -------------------------------------------------------- 01:13:04.5 --- HomeSpeed: 2000; PurgeSpeed: 2000 01:13:04.5 --- PressureTrackingExcessFactor: 96 01:13:04.5 --- FlowResistanceA: 300; FlowResistanceB: 100; FlowResistanceOut: 150 01:13:04.5 --- FC_FlowFilterDepth: 5; FC_PressureFilterDepth: 0 01:13:04.5 --- PC_OutputFilterDepth: 0 01:13:04.5 --- CompressibilityFactorB: 2.29999995231628; PressurePIDNegativeResultDivisor: 1 01:13:04.5 --- ExternalZeroPressureOffsetA: 130.617385864258; ExternalZeroPressureOffsetB: 127.978851318359 01:13:04.5 --- ExternalPressureScalingFactorA: 0.0197199992835522; ExternalPressureScalingFactorB: 0.0197199992835522 01:13:04.5 -------------------------------------------------------- 01:13:04.5 --- Final pressure channel A: 5.64780902862549 01:13:04.5 --- Final pressure channel B: 3.98738384246826 01:13:04.5 ------------------------------------------------------- 01:13:04.8 autosampler-left: MoveValveDrive(Valve Drive 2, 0 deg) 01:13:05.0 pump: Manualmode_Pump_constantSpeed(A, 0) 01:13:05.0 pump: GetSetPistonSpeed(A) --> 0 01:13:05.0 pump: Manualmode_Pump_constantSpeed(B, 0) 01:13:05.0 pump: GetSetPistonSpeed(B) --> 0 01:13:05.0 pump: IsIdle() --> True 01:13:05.1 autosampler-left: MoveValveDrive(Valve Drive 1, 60 deg) 01:13:06.8 autosampler-left: MoveToHome() 01:13:07.0 pump: IsIdle() --> True 01:13:07.0 COMPLETED