Path: 2022-07-22_10-16-48_Two-column-separation Procedure: Two column separation Argument list: uses_trap = True uses_separator = True is_isocratic = False analysis_time = 1800 oven_temperature = 50 sample_volume = 5 sample_position = Slot2:16 presample_solvent_volume = 2 presample_air_volume = 0.5 postsample_air_volume = 1 postsample_solvent_volume = 2 sample_aspirate_speed = 5 sample_postaspirate_delay = 500 sample_inject_speed = 1 calibrant_volume = 0.6 calibrantTime = 10 is_calibrant_inject = False is_extended_wash = False is_bottom_sense = True injection_method = uLPickup trap = Name: Thermo Trap Cartridge 5mm, Func: Separator, ColDia: 0.3 mm, Len: 5 mm, PartDia: 5 µm, Porosity: 42 % trap_volume = 0.148440252882118 trap_unityflow = 2.78838695282287 trap_equilibration_volumemultiplier = 10 trap_equilibration_pressure = 217.5 trap_equil_time = 0.648440252882118 separator = Name: IonOpticks 25 cm, Func: Separator, ColDia: 0.075 mm, Len: 250 mm, PartDia: 1.6 µm, Porosity: 42 % separator_volume = 0.463875790256618 separator_unityflow = 0.00058605984562038 separator_equilibration_volumemultiplier = 4 separator_equilibration_pressure = 800 column_load_volumemultiplier = 4 column_load_pressure = 217.5 separator_equil_time = 4.05402026133927 column_load_time = 0.6 gradient = (0 sec,0.3 µL/min,0.00 %), (1800 sec,0.3 µL/min,32.00 %), (2700 sec,0.3 µL/min,40.00 %), (3000 sec,0.3 µL/min,60.00 %), (3300 sec,0.3 µL/min,95.00 %), (3600 sec,0.3 µL/min,95.00 %) 00:00:00.0 STARTED 00:00:01.2 Lua version: 1.1.0 00:00:01.2 Lua date: 2020/01/30 00:00:01.3 --- Experiment: Description 00:00:01.3 ------------------------------------------------------- 00:00:01.3 --- Trap Column: Thermo Trap Cartridge 5mm 00:00:01.3 --- Manufacturer: Thermo Scientific 00:00:01.3 --- Part Number: 00:00:01.3 --- Serial Number: 00:00:01.3 --- Type/Material: µ-Precolum TM Cartridge / PepMap TM C18 00:00:01.3 --- Length: 5 mm 00:00:01.3 --- Inner Diameter: 0.3 mm 00:00:01.3 --- Particle Diameter: 5 um 00:00:01.3 --- Is Particle Pore Size: True 00:00:01.3 --- Particle Pore Size: 100 00:00:01.3 --- Is Column Porosity: False 00:00:01.3 --- Column Porosity: 0.42 (0.0-1.0) 00:00:01.3 --- Is Max Temperature: False 00:00:01.3 --- max Temperature: 65 C 00:00:01.3 --- Is Max Pressure: False 00:00:01.3 --- max Pressure: 1000 bar 00:00:01.3 --- Is Max Flow: False 00:00:01.3 --- max Flow: 9 uL/min 00:00:01.3 --- Advanced Settings: False 00:00:01.3 --- Column Comments: Manufacturer no: 160454 00:00:01.3 ------------------------------------------------------- 00:00:01.3 --- Separation Column: IonOpticks 25 cm 00:00:01.3 --- Manufacturer: IonOpticks 00:00:01.3 --- Part Number: 00:00:01.3 --- Serial Number: 00:00:01.3 --- Type/Material: C18 00:00:01.3 --- Length: 250 mm 00:00:01.3 --- Inner Diameter: 0.075 mm 00:00:01.3 --- Particle Diameter: 1.6 um 00:00:01.3 --- Is Particle Pore Size: True 00:00:01.3 --- Particle Pore Size: 120 00:00:01.3 --- Is Column Porosity: False 00:00:01.3 --- Column Porosity: 0.42 (0.0-1.0) 00:00:01.3 --- Is Max Temperature: False 00:00:01.3 --- max Temperature: 65 C 00:00:01.3 --- Is Max Pressure: True 00:00:01.3 --- max Pressure: 1000 bar 00:00:01.3 --- Is Max Flow: False 00:00:01.3 --- max Flow: 9 uL/min 00:00:01.3 --- Advanced Settings: False 00:00:01.3 --- Column Comments: 00:00:01.3 ------------------------------------------------------- 00:00:01.3 'Valve Drive 1' is 'ILcInjectorValve' 00:00:01.3 'Valve Drive 2' is 'ISelectorValve' 00:00:01.3 --- Trap equil time: 0.64 00:00:01.3 --- Seperator equil time: 4.05 00:00:01.3 --- Column loading time: 0.6 00:00:01.3 pump: GetSettings() --> PumpSettings 00:00:01.3 -------------------------------------------------------- 00:00:01.3 --- Log instrument settings called from: Two Column Separation 00:00:01.3 -------------------------------------------------------- 00:00:01.3 --- HomeSpeed: 2000; PurgeSpeed: 2000 00:00:01.3 --- PressureTrackingExcessFactor: 96 00:00:01.3 --- FlowResistanceA: 300; FlowResistanceB: 100; FlowResistanceOut: 150 00:00:01.3 --- FC_FlowFilterDepth: 5; FC_PressureFilterDepth: 0 00:00:01.3 --- PC_OutputFilterDepth: 0 00:00:01.3 --- CompressibilityFactorB: 2.29999995231628; PressurePIDNegativeResultDivisor: 1 00:00:01.3 --- ExternalZeroPressureOffsetA: 131.015731811523; ExternalZeroPressureOffsetB: 127.931526184082 00:00:01.3 --- ExternalPressureScalingFactorA: 0.0197199992835522; ExternalPressureScalingFactorB: 0.0197199992835522 00:00:01.3 -------------------------------------------------------- 00:00:01.3 --- Oven temperature: 50 00:00:01.3 --- Separator: Name: IonOpticks 25 cm, Func: Separator, ColDia: 0.075 mm, Len: 250 mm, PartDia: 1.6 µm, Porosity: 42 % --- 00:00:01.3 --- Trap: Name: Thermo Trap Cartridge 5mm, Func: Separator, ColDia: 0.3 mm, Len: 5 mm, PartDia: 5 µm, Porosity: 42 % --- 00:00:01.3 --- MaxPressureLimitAB: 1000 bar --- 00:00:01.3 pump: GetCurrentPressure(A) --> 6.294623 00:00:01.3 pump: GetCurrentPressure(B) --> 6.815229 00:00:01.3 pump: GetSettings() --> PumpSettings 00:00:01.3 pump: GetSetManualValvePosition(A) --> 240 00:00:01.3 pump: GetSetManualValvePosition(B) --> 240 00:00:01.3 ------------------------------------------------------- 00:00:01.3 --- Reduce pressure --- 00:00:01.3 ------------------------------------------------------- 00:00:01.3 --- Pressure channel A: 6.29462337493896 00:00:01.3 --- Pressure channel B: 6.8152289390564 00:00:01.3 pump: Manualmode_Pump_constantSpeed(A, 0) 00:00:01.3 pump: GetSetPistonSpeed(A) --> 0 00:00:01.3 pump: Manualmode_Pump_constantSpeed(B, 0) 00:00:01.3 pump: GetSetPistonSpeed(B) --> 0 00:00:01.3 pump: IsIdle() --> True 00:00:01.3 ------------------------------------------------------- 00:00:01.4 pump: SetMaxPressureLimit(A, 1020) 00:00:01.4 pump: GetMaxPressureLimit(A) --> 1020 00:00:01.5 pump: SetMaxPressureLimit(B, 1020) 00:00:01.5 pump: GetMaxPressureLimit(B) --> 1020 00:00:01.5 pump: GetSettings() --> PumpSettings 00:00:01.5 ------------------------------------------------------------------ 00:00:01.5 PID settings changed: 00:00:01.5 ------------------------------------------------------------------ 00:00:01.5 P: 175 : True; Pmin: -5000 : True; Pmax: 5000 : True 00:00:01.5 I: 5 : True; Imin: -200 : True; Imax: 1000 : True; IWindow: 10 : True 00:00:01.5 D: 0 : True; Dmin: -1000 : True; Dmax: 1000 : True 00:00:01.5 Result min: -6000 : True; Result max: 8000 : True 00:00:01.5 ------------------------------------------------------------------ 00:00:01.5 pump: SetSettings(PumpSettings) 00:00:01.5 pump: GetPumpSideState(A) --> manualMode 00:00:01.5 pump: GetPumpSideState(B) --> manualMode 00:00:01.5 pump: GetPistonPosition(A) --> 169.474472334963 00:00:01.5 pump: GetPistonPosition(B) --> 51.559190312185 00:00:01.5 ------------------------------------------------------- 00:00:01.5 --- Optional Refill of Pump Heads: 00:00:01.5 --- initiated by channel A: False, B: False 00:00:01.5 pump: GetPistonPosition(A) --> 169.474472334963 00:00:01.5 pump: GetPistonPosition(B) --> 51.559190312185 00:00:01.5 --- Piston position channel A: 169.474472334963, B: 51.559190312185 00:00:01.5 ------------------------------------------------------- 00:00:01.5 pump: SetDigitalOutput(PO2, False) 00:00:01.5 pump: GetCurrentPressure(A) --> 6.255183 00:00:01.5 pump: GetCurrentPressure(B) --> 6.815229 00:00:01.5 pump: GetSettings() --> PumpSettings 00:00:01.5 pump: GetSetManualValvePosition(A) --> 240 00:00:01.5 pump: GetSetManualValvePosition(B) --> 240 00:00:01.5 ------------------------------------------------------- 00:00:01.5 --- Reduce pressure --- 00:00:01.5 ------------------------------------------------------- 00:00:01.5 --- Pressure channel A: 6.25518321990967 00:00:01.5 --- Pressure channel B: 6.8152289390564 00:00:01.5 pump: GetSettings() --> PumpSettings 00:00:01.5 ------------------------------------------------------------------ 00:00:01.5 PID settings changed: 00:00:01.5 ------------------------------------------------------------------ 00:00:01.5 P: 250 : True; Pmin: -5500 : True; Pmax: 32000 : True 00:00:01.5 I: 50 : True; Imin: -20000 : True; Imax: 20000 : True; IWindow: 50 : True 00:00:01.5 D: 1500 : True; Dmin: -10000 : True; Dmax: 10000 : True 00:00:01.5 Result min: -32000 : True; Result max: 32000 : True 00:00:01.5 ------------------------------------------------------------------ 00:00:01.5 pump: SetSettings(PumpSettings) 00:00:01.7 --- Set pressure channel A: 10 00:00:01.7 --- Set pressure channel B: 5 00:00:01.7 --- Reduce pressure flow controlled: True 00:00:03.0 -------------------------------------------------------- 00:00:03.0 --- Log PID parameter called from: reducePressure 00:00:03.0 -------------------------------------------------------- 00:00:03.0 --- P: 175; Pmin: -5000; Pmax: 5000 00:00:03.0 --- I: 5; Imin: -200; Imax: 1000; IWindow: 10 00:00:03.0 --- D: 0; Dmin: -1000; Dmax: 1000 00:00:03.0 --- Result min: -6000; Result max: 8000 00:00:03.0 -------------------------------------------------------- 00:00:03.0 -------------------------------------------------------- 00:00:03.0 --- Log instrument settings called from: reducePressure 00:00:03.0 -------------------------------------------------------- 00:00:03.0 --- HomeSpeed: 2000; PurgeSpeed: 2000 00:00:03.0 --- PressureTrackingExcessFactor: 96 00:00:03.0 --- FlowResistanceA: 300; FlowResistanceB: 100; FlowResistanceOut: 150 00:00:03.0 --- FC_FlowFilterDepth: 5; FC_PressureFilterDepth: 0 00:00:03.0 --- PC_OutputFilterDepth: 0 00:00:03.0 --- CompressibilityFactorB: 2.29999995231628; PressurePIDNegativeResultDivisor: 1 00:00:03.0 --- ExternalZeroPressureOffsetA: 131.015731811523; ExternalZeroPressureOffsetB: 127.931526184082 00:00:03.0 --- ExternalPressureScalingFactorA: 0.0197199992835522; ExternalPressureScalingFactorB: 0.0197199992835522 00:00:03.0 -------------------------------------------------------- 00:00:03.0 --- Final pressure channel A: 6.25518321990967 00:00:03.0 --- Final pressure channel B: 0 00:00:03.0 ------------------------------------------------------- 00:00:03.0 pump: GetSetManualValvePosition(A) --> 240 00:00:03.0 pump: GetCurrentPressure(A) --> 6.255183 00:00:03.0 pump: SetManualValvePosition(A, 0, True) 00:00:03.6 pump: GetSetManualValvePosition(B) --> 240 00:00:03.6 pump: GetCurrentPressure(B) --> 0 00:00:03.6 pump: SetManualValvePosition(B, 180, False) 00:00:04.0 pump: GetSettings() --> PumpSettings 00:00:04.0 ------------------------------------------------------------------ 00:00:04.0 PID settings changed: 00:00:04.0 ------------------------------------------------------------------ 00:00:04.0 P: 250 : True; Pmin: -10000 : True; Pmax: 10000 : True 00:00:04.0 I: 20 : True; Imin: -10000 : True; Imax: 20000 : True; IWindow: 10 : True 00:00:04.0 D: 1500 : True; Dmin: -10000 : True; Dmax: 10000 : True 00:00:04.0 Result min: -10000 : True; Result max: 32000 : True 00:00:04.0 ------------------------------------------------------------------ 00:00:04.0 pump: SetSettings(PumpSettings) 00:00:04.0 status: SetStatus(equilibrate separation-column, this finishes at about: 4.85 min., ) 00:00:04.6 autosampler-none: MoveValveDrive(Valve Drive 2, 180 deg) 00:00:04.6 --- MaxPressureLimitA: 800 bar --- 00:00:04.6 pump: GetCurrentPressure(A) --> 6.294623 00:00:04.6 pump: GetCurrentPressure() --> 6.294623 00:00:04.6 pump: GetSettings() --> PumpSettings 00:00:04.6 pump: GetSetManualValvePosition(A) --> 0 00:00:04.6 ------------------------------------------------------- 00:00:04.6 --- Reduce pressure --- 00:00:04.6 ------------------------------------------------------- 00:00:04.6 --- Pressure channel A: 6.29462337493896 00:00:04.6 --- Pressure channel B: 6.29462337493896 00:00:04.6 pump: Manualmode_Pump_constantSpeed(A, 0) 00:00:04.6 pump: GetSetPistonSpeed(A) --> 0 00:00:04.6 pump: Manualmode_Pump_constantSpeed(, 0) 00:00:04.6 pump: GetSetPistonSpeed() --> 0 00:00:04.6 pump: IsIdle() --> True 00:00:04.6 ------------------------------------------------------- 00:00:04.7 pump: SetMaxPressureLimit(A, 820) 00:00:04.7 pump: GetMaxPressureLimit(A) --> 820 00:00:04.7 ------------------------------------------------------- 00:00:04.7 --- Separation Column Equilibration: 00:00:04.7 ------------------------------------------------------- 00:00:04.7 --- ovenTemp [C]: 50 00:00:04.7 --- Separator Unity-Flow @ ovenTemp [ul/min/bar]: 0.00058605984562038 00:00:04.7 --- Separator Volume [ul]: 0.463875790256618 00:00:04.7 --- Separator Equilibration Volume Multiplier: 4 00:00:04.7 --- Separator Equilibration Pressure [bar]: 800 00:00:04.7 --- Separator Equilibration Flow [ul/min]: 0.457694594849779 00:00:04.7 --- Separator Equilibration Volume [ul]: 1.85550316102647 00:00:04.7 --- Separator Equilibration Time [min]: 4.05402026133927 00:00:04.7 --- max. Volume (10*V+2ul/min*t) [ul]: 26.6630721329433 00:00:04.7 --- Separator Equilibration Time Out [min]: 40.5402026133927 00:00:04.7 ------------------------------------------------------- 00:00:04.7 pump: Manualmode_Pump_constantPressure(A, 800) 00:00:04.7 pump: GetPistonPosition(A) --> 169.473215697901 00:00:04.7 --- Injection delay: 237.164855895902s 00:00:29.7 pump: GetPistonPosition(A) --> 253.464533049665 00:00:29.7 pump: GetPistonPosition(A) --> 253.464533049665 00:00:29.7 pump: GetPistonPosition(A) --> 253.464533049665 00:04:02.6 status: SetStatus(aspirate & inject, ) 00:04:02.8 autosampler-left: LeaveObject() 00:04:07.2 autosampler-left: MoveToObject(LCMS Wash 1, 3) 00:04:07.9 autosampler-left: PenetrateObject(LCMS Wash 1, 3) 00:04:08.0 autosampler-left: EmptySyringe() 00:04:08.8 autosampler-left: LeaveObject() 00:04:09.7 Cleaning syringe and needle exterior 00:04:25.5 autosampler-left: CleanSyringe(LCMS Wash 1, 1, 30 mm, 40 uL/s, 500 ms, 30 mm, 40 uL/s, 80 %, 2) 00:04:25.6 autosampler-left: ChangeTool(LCP 1) --> False 00:04:28.7 autosampler-left: MoveToObject(Item Position 3, 1, True, True, True) 00:04:31.9 autosampler-left: PenetrateWithBottomSense(Item Position 3, 1, 1 mm) 00:04:32.9 autosampler-left: AspirateSyringe(2 uL, 5 uL/s, 500 ms) 00:04:33.0 pump: GetPistonPosition(A) --> 256.722364631438 00:04:33.0 pump: GetCurrentPressure(A) --> 800.1429 00:04:33.0 --- act. pressure [bar]: 800.142944335938 00:04:33.0 --- used volume [uL]: 3.25783158177262 00:04:33.0 --- average flow (incl. leaks) [uL/min]: 0.803424203054244 00:04:33.0 --- act. equilibration time [min]: 4.05493333333482 00:04:33.0 ------------------------------------------------------- 00:04:33.0 status: RemoveStatus(equilibrate separation-column, this finishes at about: 4.85 min.) 00:04:33.0 status: SetStatus(equilibrate trap-column, this finishes at about: 5.67 min., ) 00:04:33.1 --- MaxPressureLimitA: 217.5 bar --- 00:04:33.1 pump: GetCurrentPressure(A) --> 800.1232 00:04:33.1 pump: GetCurrentPressure() --> 800.1232 00:04:33.1 pump: GetSettings() --> PumpSettings 00:04:33.1 pump: GetSetManualValvePosition(A) --> 0 00:04:33.1 ------------------------------------------------------- 00:04:33.1 --- Reduce pressure --- 00:04:33.1 ------------------------------------------------------- 00:04:33.1 --- Pressure channel A: 800.123168945313 00:04:33.1 --- Pressure channel B: 800.123168945313 00:04:33.1 pump: GetSettings() --> PumpSettings 00:04:33.1 ------------------------------------------------------------------ 00:04:33.1 PID settings changed: 00:04:33.1 ------------------------------------------------------------------ 00:04:33.1 P: 250 : True; Pmin: -32000 : True; Pmax: 32000 : True 00:04:33.1 I: 20 : True; Imin: -20000 : True; Imax: 20000 : True; IWindow: 100 : True 00:04:33.1 D: 1500 : True; Dmin: -10000 : True; Dmax: 10000 : True 00:04:33.1 Result min: -32000 : True; Result max: 32000 : True 00:04:33.1 ------------------------------------------------------------------ 00:04:33.1 pump: SetSettings(PumpSettings) 00:04:33.2 --- Set pressure channel A: 217.5 00:04:33.2 --- Set pressure channel B: 217.5 00:04:33.2 --- Reduce pressure flow controlled: False 00:04:33.8 autosampler-left: LeaveObject() 00:04:34.8 autosampler-left: AspirateSyringe(0.5 uL, 5 uL/s, 500 ms) 00:04:45.2 autosampler-left: MoveToObject(Rack 2, 16, True, True, True) 00:04:49.0 autosampler-left: PenetrateWithBottomSense(Rack 2, 16, 1 mm) 00:04:49.2 -------------------------------------------------------- 00:04:49.2 --- Log PID parameter called from: reducePressure 00:04:49.2 -------------------------------------------------------- 00:04:49.2 --- P: 250; Pmin: -10000; Pmax: 10000 00:04:49.2 --- I: 20; Imin: -10000; Imax: 20000; IWindow: 10 00:04:49.2 --- D: 1500; Dmin: -10000; Dmax: 10000 00:04:49.2 --- Result min: -10000; Result max: 32000 00:04:49.2 -------------------------------------------------------- 00:04:49.2 -------------------------------------------------------- 00:04:49.2 --- Log instrument settings called from: reducePressure 00:04:49.2 -------------------------------------------------------- 00:04:49.2 --- HomeSpeed: 2000; PurgeSpeed: 2000 00:04:49.2 --- PressureTrackingExcessFactor: 96 00:04:49.2 --- FlowResistanceA: 300; FlowResistanceB: 100; FlowResistanceOut: 150 00:04:49.2 --- FC_FlowFilterDepth: 5; FC_PressureFilterDepth: 0 00:04:49.2 --- PC_OutputFilterDepth: 0 00:04:49.2 --- CompressibilityFactorB: 2.29999995231628; PressurePIDNegativeResultDivisor: 1 00:04:49.2 --- ExternalZeroPressureOffsetA: 131.015731811523; ExternalZeroPressureOffsetB: 127.931526184082 00:04:49.2 --- ExternalPressureScalingFactorA: 0.0197199992835522; ExternalPressureScalingFactorB: 0.0197199992835522 00:04:49.2 -------------------------------------------------------- 00:04:49.2 --- Final pressure channel A: 216.194290161133 00:04:49.2 --- Final pressure channel B: 216.194290161133 00:04:49.2 ------------------------------------------------------- 00:04:49.3 pump: SetMaxPressureLimit(A, 237.5) 00:04:49.3 pump: GetMaxPressureLimit(A) --> 237.5 00:04:49.4 autosampler-none: MoveValveDrive(Valve Drive 2, 60 deg) 00:04:49.4 ------------------------------------------------------- 00:04:49.4 --- Trap Column Equilibration 00:04:49.4 ------------------------------------------------------- 00:04:49.4 --- Trap Temperature: fixed to 20 C 00:04:49.4 --- Trap Unity-Flow @ 20C [ul/bar]: 2.78838695282287 00:04:49.4 --- Trap Volume [ul]: 0.148440252882118 00:04:49.4 --- Trap Equilibration Volume Multiplier: 10 00:04:49.4 --- Trap Equilibration Pressure [bar]: 217.5 00:04:49.4 --- Trap Equilibration Flow [ul/min]: 9.99557465783688 00:04:49.4 --- Trap Equilibration Volume (+5) [ul]: 6.48440252882118 00:04:49.4 --- Trap Equilibration Time [min]: 0.648727336925764 00:04:49.4 --- max. Volume (10*V+2ul/min*t) [ul]: 66.1414799620633 00:04:49.4 ------------------------------------------------------- 00:04:49.4 pump: Manualmode_Pump_constantPressure(A, 217.5) 00:04:49.4 pump: GetPistonPosition(A) --> 197.328670559731 00:04:50.6 autosampler-left: AspirateSyringe(5 uL, 5 uL/s, 500 ms) 00:04:58.4 autosampler-left: LeaveObject() 00:04:59.2 autosampler-left: AspirateSyringe(1 uL, 5 uL/s, 500 ms) 00:05:02.9 autosampler-left: MoveToObject(LCMS Wash 1, 1, True, True, True) 00:05:03.8 autosampler-left: PenetrateObject(LCMS Wash 1, 1, 30 mm) 00:05:08.8 autosampler-left: Wait(5 s) 00:05:09.8 autosampler-left: LeaveObject() 00:05:11.5 pump: GetPistonPosition(A) --> 200.30313048415 00:05:11.5 pump: GetPistonPosition(A) --> 200.30313048415 00:05:11.5 pump: GetPistonPosition(A) --> 200.30313048415 00:05:12.8 autosampler-left: MoveToObject(LCInjector1) 00:05:13.7 autosampler-left: DispenseSyringe(0.75 uL, 1 uL/s) 00:05:16.2 autosampler-left: PenetrateWithConstForce(LCInjector1) 00:05:16.3 autosampler-left: MoveValveDrive(Valve Drive 1, 0 deg) 00:05:17.3 autosampler-left: Wait(1000 ms) 00:05:24.2 autosampler-left: DispenseSyringe(6.75 uL, 1 uL/s) 00:05:25.2 autosampler-left: Wait(1000 ms) 00:05:26.2 status: RemoveStatus(aspirate & inject) 00:05:51.2 pump: GetPistonPosition(A) --> 205.607395520471 00:05:51.2 pump: GetPistonPosition(A) --> 205.607395520471 00:06:00.2 pump: GetPistonPosition(A) --> 206.804551760999 00:06:00.2 pump: GetCurrentPressure(A) --> 217.3381 00:06:00.2 ------------------------------------------------------- 00:06:00.2 --- Finished: Trap Column Equilibration (const_pressure_load_and_equilibration) 00:06:00.2 ------------------------------------------------------- 00:06:00.2 --- act. pressure [bar]: 217.33805847168 00:06:00.2 --- used volume [uL]: 6.50142127684896 00:06:00.2 --- average flow [uL/min]: 8.00503338007566 00:06:00.2 --- act. equilibration time [min]: 0.812166666666356 00:06:00.2 ------------------------------------------------------- 00:06:00.2 status: RemoveStatus(equilibrate trap-column, this finishes at about: 5.67 min.) 00:06:02.2 --- MaxPressureLimitA: 217.5 bar --- 00:06:02.2 pump: GetCurrentPressure(A) --> 217.2789 00:06:02.2 pump: GetCurrentPressure() --> 217.2789 00:06:02.2 pump: GetSettings() --> PumpSettings 00:06:02.2 pump: GetSetManualValvePosition(A) --> 0 00:06:02.2 ------------------------------------------------------- 00:06:02.2 --- Reduce pressure --- 00:06:02.2 ------------------------------------------------------- 00:06:02.2 --- Pressure channel A: 217.278900146484 00:06:02.2 --- Pressure channel B: 217.278900146484 00:06:02.2 pump: Manualmode_Pump_constantSpeed(A, 0) 00:06:02.2 pump: GetSetPistonSpeed(A) --> 0 00:06:02.2 pump: Manualmode_Pump_constantSpeed(, 0) 00:06:02.2 pump: GetSetPistonSpeed() --> 0 00:06:02.2 pump: IsIdle() --> False 00:06:02.2 ------------------------------------------------------- 00:06:02.3 pump: SetMaxPressureLimit(A, 237.5) 00:06:02.3 pump: GetMaxPressureLimit(A) --> 237.5 00:06:02.3 status: SetStatus(load trap-column, this finishes at about: 8.30 min., ) 00:06:02.4 autosampler-left: MoveValveDrive(Valve Drive 1, 60 deg) 00:06:03.8 autosampler-left: EmptySyringe(5 uL/s) 00:06:03.9 ------------------------------------------------------- 00:06:03.9 --- Sample Loading on Trap Column 00:06:03.9 ------------------------------------------------------- 00:06:03.9 --- Trap Temperature: fixed to 20 C 00:06:03.9 --- Trap Unity-Flow [ul/bar]: 2.78838695282287 00:06:03.9 --- Trap Volume [ul]: 0.148440252882118 00:06:03.9 --- Sample Volume [ul]: 5 00:06:03.9 --- Sample Volume Multiplier: 4 00:06:03.9 --- Trap Loading Pressure [bar]: 217.5 00:06:03.9 --- Trap Loading Flow [uL/min]: 9.99557465783688 00:06:03.9 --- Trap Loading Volume (+2) [uL]: 22 00:06:03.9 --- Trap Loading Time [min]: 2.200974006307 00:06:03.9 --- max. Volume (10*V+2ul/min*t) [ul]: 224.401948012614 00:06:03.9 ------------------------------------------------------- 00:06:03.9 pump: Manualmode_Pump_constantPressure(A, 217.5) 00:06:03.9 pump: GetPistonPosition(A) --> 207.084572386189 00:06:23.2 pump: GetPistonPosition(A) --> 210.331094234408 00:06:23.2 pump: GetPistonPosition(A) --> 210.331094234408 00:06:23.2 pump: GetPistonPosition(A) --> 210.331094234408 00:07:02.8 autosampler-left: CleanInjector(LCInjector1, LCP 1, 200 uL, 0 mm, 5 uL/s, 5 s, 20 mm, 40 uL/s, 1000 ms, 2, False) 00:08:03.6 autosampler-left: CleanInjector(LCInjector1, LCP 1, 200 uL, 0 mm, 5 uL/s, 5 s, 20 mm, 40 uL/s, 1000 ms, 1, True) 00:08:18.9 autosampler-left: CleanSyringe(LCMS Wash 1, 2, 30 mm, 40 uL/s, 500 ms, 30 mm, 40 uL/s, 80 %, 2) 00:08:35.3 pump: GetPistonPosition(A) --> 227.956266779088 00:08:35.3 pump: GetPistonPosition(A) --> 227.956266779088 00:08:39.7 autosampler-left: CleanSyringe(LCMS Wash 1, 1, 30 mm, 40 uL/s, 500 ms, 30 mm, 40 uL/s, 80 %, 3) 00:08:43.4 autosampler-left: MoveToHome() 00:09:08.0 pump: GetPistonPosition(A) --> 232.363502393054 00:09:08.0 pump: GetCurrentPressure(A) --> 217.4761 00:09:08.0 ------------------------------------------------------- 00:09:08.0 --- Finished: Sample Loading on Trap Column (const_pressure_load_and_equilibration) 00:09:08.0 ------------------------------------------------------- 00:09:08.0 --- act. pressure [bar]: 217.476089477539 00:09:08.0 --- used volume [uL]: 22.0324081586457 00:09:08.0 --- average flow [uL/min]: 8.01771303338549 00:09:08.0 --- act. loading time [min]: 2.7479666666671 00:09:08.0 ------------------------------------------------------- 00:09:08.0 --- Separator: Name: IonOpticks 25 cm, Func: Separator, ColDia: 0.075 mm, Len: 250 mm, PartDia: 1.6 µm, Porosity: 42 % --- 00:09:08.0 --- Trap: Name: Thermo Trap Cartridge 5mm, Func: Separator, ColDia: 0.3 mm, Len: 5 mm, PartDia: 5 µm, Porosity: 42 % --- 00:09:08.0 --- MaxPressureLimitAB: 1000 bar --- 00:09:08.0 pump: GetCurrentPressure(A) --> 217.4761 00:09:08.0 pump: GetCurrentPressure(B) --> 0 00:09:08.0 pump: GetSettings() --> PumpSettings 00:09:08.0 pump: GetSetManualValvePosition(A) --> 0 00:09:08.0 ------------------------------------------------------- 00:09:08.0 --- Reduce pressure --- 00:09:08.0 ------------------------------------------------------- 00:09:08.0 --- Pressure channel A: 217.476089477539 00:09:08.0 --- Pressure channel B: 0 00:09:08.0 pump: Manualmode_Pump_constantSpeed(A, 0) 00:09:08.1 pump: GetSetPistonSpeed(A) --> 0 00:09:08.1 pump: Manualmode_Pump_constantSpeed(B, 0) 00:09:08.1 pump: GetSetPistonSpeed(B) --> 0 00:09:08.1 pump: IsIdle() --> False 00:09:08.1 ------------------------------------------------------- 00:09:08.2 pump: SetMaxPressureLimit(A, 1020) 00:09:08.2 pump: GetMaxPressureLimit(A) --> 1020 00:09:08.3 pump: SetMaxPressureLimit(B, 1020) 00:09:08.3 pump: GetMaxPressureLimit(B) --> 1020 00:09:08.3 status: RemoveStatus(load trap-column, this finishes at about: 8.30 min.) 00:09:08.3 pump: GetSettings() --> PumpSettings 00:09:08.3 ------------------------------------------------------------------ 00:09:08.3 PID settings changed: 00:09:08.3 ------------------------------------------------------------------ 00:09:08.3 P: 100 : True; Pmin: -10000 : True; Pmax: 10000 : True 00:09:08.3 I: 100 : True; Imin: -1000 : True; Imax: 1000 : True; IWindow: 100 : True 00:09:08.3 D: 5000 : True; Dmin: -32000 : True; Dmax: 32000 : True 00:09:08.3 Result min: -5000 : True; Result max: 25000 : True 00:09:08.3 ------------------------------------------------------------------ 00:09:08.3 pump: SetSettings(PumpSettings) 00:09:08.3 pump: GetSettings() --> PumpSettings 00:09:08.3 ------------------------------------------------------------------ 00:09:08.3 PID settings changed: 00:09:08.3 ------------------------------------------------------------------ 00:09:08.3 P: 100 : False; Pmin: -10000 : False; Pmax: 10000 : False 00:09:08.3 I: 100 : False; Imin: -1000 : False; Imax: 1000 : False; IWindow: 2 : True 00:09:08.3 D: 5000 : False; Dmin: -32000 : False; Dmax: 32000 : False 00:09:08.3 Result min: -5000 : False; Result max: 25000 : False 00:09:08.3 ------------------------------------------------------------------ 00:09:08.3 pump: SetSettings(PumpSettings) 00:09:08.3 status: SetStatus(preparing gradient start, ) 00:09:08.3 --- Starting conditioning paths --- 00:09:08.3 pump: GetSettings() --> PumpSettings 00:09:08.3 ------------------------------------------------------------------ 00:09:08.3 PID settings changed: 00:09:08.3 ------------------------------------------------------------------ 00:09:08.3 P: 100 : True; Pmin: -10000 : True; Pmax: 10000 : True 00:09:08.3 I: 100 : True; Imin: -1000 : True; Imax: 1000 : True; IWindow: 100 : True 00:09:08.3 D: 5000 : True; Dmin: -32000 : True; Dmax: 32000 : True 00:09:08.3 Result min: -5000 : True; Result max: 25000 : True 00:09:08.3 ------------------------------------------------------------------ 00:09:08.3 pump: SetSettings(PumpSettings) 00:09:08.4 pump: Manualmode_Pump_constantPressure(A, 0) 00:09:08.4 pump: GetTargetPressure(A) --> 0 00:09:08.4 pump: GetCurrentPressure(A) --> 217.3183 00:09:09.4 pump: GetCurrentPressure(A) --> 203.3171 00:09:10.4 pump: GetCurrentPressure(A) --> 183.7549 00:09:11.4 pump: GetCurrentPressure(A) --> 164.4293 00:09:12.4 pump: GetCurrentPressure(A) --> 145.0445 00:09:13.4 pump: GetCurrentPressure(A) --> 125.3245 00:09:14.4 pump: GetCurrentPressure(A) --> 106.2947 00:09:15.4 pump: GetCurrentPressure(A) --> 87.30438 00:09:16.4 pump: GetCurrentPressure(A) --> 68.51122 00:09:17.4 pump: GetCurrentPressure(A) --> 52.43942 00:09:18.4 pump: GetCurrentPressure(A) --> 35.24358 00:09:19.4 pump: GetCurrentPressure(A) --> 21.53818 00:09:20.4 pump: GetCurrentPressure(A) --> 13.68962 00:09:20.5 autosampler-none: MoveValveDrive(Valve Drive 2, 0 deg) 00:09:20.5 pump: SetManualValvePosition(A, 240, False) 00:09:21.1 pump: SetManualValvePosition(B, 240, False) 00:09:23.1 --- Set constant binary flow --- 00:09:23.2 pump: Manualmode_Pump_constantFlow_binary(1, 1) 00:09:23.2 pump: GetSetFlow(A) --> 1 00:09:23.2 pump: GetSetFlow(B) --> 1 00:09:23.2 00:09:23.2 --- Starting spline flow reduction B/A --- 00:09:23.2 pump: GetSetExternalTemperature() --> 50 00:09:23.2 --- Oven temperatur: 50 00:09:23.2 --- Separator: Name: IonOpticks 25 cm, Func: Separator, ColDia: 0.075 mm, Len: 250 mm, PartDia: 1.6 µm, Porosity: 42 % 00:09:23.2 --- Spline fitting time: 97.6577595902957 s 00:09:23.2 --- Spline fitting time adapted: 10 s 00:09:23.2 --- y = 0x + 1 00:09:23.2 --- p0.Time -1, p0.Flow 1 00:09:23.2 --- p1.Time 0, p1.Flow 1 00:09:23.2 --- p2.Time 9, p2.Flow 1 00:09:23.2 --- p3.Time 10, p3.Flow 1, p3.Mix 0 00:09:23.2 --- p4.Time 40, p4.Flow 1, p4.Mix 0 00:09:23.2 --- Strategy function end 00:09:23.2 --- y = 0x + 0 00:09:23.2 --- p0.Time -1, p0.Flow 1 00:09:23.2 --- p1.Time 0, p1.Flow 1 00:09:23.2 --- p2.Time 9, p2.Flow 0 00:09:23.2 --- p3.Time 10, p3.Flow 0, p3.Mix 0 00:09:23.2 --- p4.Time 40, p4.Flow 0, p4.Mix 0 00:09:23.2 --- Strategy function end 00:09:23.2 xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx 00:09:23.3 XXXXX Time 0, Flow 2, Mix 50 00:09:23.3 XXXXX Time 1, Flow 1.94545, Mix 48.59813 00:09:23.3 XXXXX Time 2, Flow 1.84848, Mix 45.90163 00:09:23.4 XXXXX Time 3, Flow 1.72121, Mix 41.9014 00:09:23.4 XXXXX Time 4, Flow 1.57575, Mix 36.53846 00:09:23.5 XXXXX Time 5, Flow 1.42424, Mix 29.78723 00:09:23.5 XXXXX Time 6, Flow 1.27878, Mix 21.80094 00:09:23.5 XXXXX Time 7, Flow 1.15151, Mix 13.15789 00:09:23.6 XXXXX Time 8, Flow 1.05454, Mix 5.17241 00:09:23.6 XXXXX Time 9, Flow 1, Mix 0 00:09:23.6 --- Gradient is created, switching valves A and B 00:09:23.6 pump: GetSetManualValvePosition(A) --> 240 00:09:23.6 pump: GetCurrentPressure(A) --> 7.556703 00:09:23.6 pump: SetManualValvePosition(A, 240, False) 00:09:23.8 pump: GetSetManualValvePosition(B) --> 240 00:09:23.8 pump: GetCurrentPressure(B) --> 3.344509 00:09:23.8 pump: SetManualValvePosition(B, 240, False) 00:09:24.0 --- Valves A and B switched to MixTee, starting gradient 00:09:24.0 --- LoadGradient() --- 00:09:24.0 --- Loading gradient method index: 0 00:09:24.0 pump: LoadMethod(0, ZrData.ZrPumpMethod) 00:09:24.5 pump: GetMethodState(0) --> loaded 00:09:24.5 --- Set gradient start 00:09:24.5 --- StartGradient() --- 00:09:24.5 --- Starting gradient method index: 0 00:09:24.5 pump: SetMethodSignal(0, StartTrunk) 00:09:24.7 pump: GetMethodState(0) --> runningTrunk 00:09:24.7 --- Start GradientSplineFit 00:09:24.7 pump: GetMethodState(0) --> runningTrunk 00:09:25.7 pump: GetCurrentPressure(A) --> 81.25034 00:09:25.7 pump: GetCurrentPressure(B) --> 42.98171 00:09:25.7 --- GradientSplineFit is running 00:09:33.7 --- GradientSplineFit has finished 00:09:33.7 --- End GradientSplineFit 00:09:33.7 --- Finished spline flow reduction B --- 00:09:34.8 pump: GetSetExternalTemperature() --> 50 00:09:34.8 --- Oven temperatur: 50 00:09:34.8 --- Separator: Name: IonOpticks 25 cm, Func: Separator, ColDia: 0.075 mm, Len: 250 mm, PartDia: 1.6 µm, Porosity: 42 % 00:09:34.8 --- Spline fitting time: 97.6577595902957 s 00:09:34.8 --- Spline fitting time adapted: 60 s 00:09:34.8 --- y = 0x + 0.01 00:09:34.8 --- p0.Time -1, p0.Flow 1 00:09:34.8 --- p1.Time 0, p1.Flow 1 00:09:34.8 --- p2.Time 59, p2.Flow 0.01 00:09:34.8 --- p3.Time 60, p3.Flow 0.01, p3.Mix 0 00:09:34.8 --- p4.Time 90, p4.Flow 0.01, p4.Mix 0 00:09:34.8 --- Strategy function end 00:09:34.8 --- y = 0x + 0 00:09:34.8 --- p0.Time -1, p0.Flow 0 00:09:34.8 --- p1.Time 0, p1.Flow 0 00:09:34.8 --- p2.Time 59, p2.Flow 0 00:09:34.8 --- p3.Time 60, p3.Flow 0, p3.Mix 0 00:09:34.8 --- p4.Time 90, p4.Flow 0, p4.Mix 0 00:09:34.8 --- Strategy function end 00:09:34.8 xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx 00:09:34.8 XXXXX Time 0, Flow 1, Mix 0 00:09:34.9 XXXXX Time 1, Flow 0.99837, Mix 0 00:09:34.9 XXXXX Time 2, Flow 0.99518, Mix 0 00:09:34.9 XXXXX Time 3, Flow 0.99048, Mix 0 00:09:35.0 XXXXX Time 4, Flow 0.98432, Mix 0 00:09:35.0 XXXXX Time 5, Flow 0.97675, Mix 0 00:09:35.1 XXXXX Time 6, Flow 0.96784, Mix 0 00:09:35.1 XXXXX Time 7, Flow 0.95763, Mix 0 00:09:35.1 XXXXX Time 8, Flow 0.94619, Mix 0 00:09:35.2 XXXXX Time 9, Flow 0.93356, Mix 0 00:09:35.2 XXXXX Time 10, Flow 0.91981, Mix 0 00:09:35.3 XXXXX Time 11, Flow 0.90498, Mix 0 00:09:35.3 XXXXX Time 12, Flow 0.88914, Mix 0 00:09:35.3 XXXXX Time 13, Flow 0.87233, Mix 0 00:09:35.4 XXXXX Time 14, Flow 0.85462, Mix 0 00:09:35.4 XXXXX Time 15, Flow 0.83605, Mix 0 00:09:35.5 XXXXX Time 16, Flow 0.81668, Mix 0 00:09:35.5 XXXXX Time 17, Flow 0.79658, Mix 0 00:09:35.6 XXXXX Time 18, Flow 0.77578, Mix 0 00:09:35.6 XXXXX Time 19, Flow 0.75435, Mix 0 00:09:35.7 XXXXX Time 20, Flow 0.73235, Mix 0 00:09:35.7 XXXXX Time 21, Flow 0.70982, Mix 0 00:09:35.8 XXXXX Time 22, Flow 0.68682, Mix 0 00:09:35.8 XXXXX Time 23, Flow 0.66341, Mix 0 00:09:35.8 XXXXX Time 24, Flow 0.63964, Mix 0 00:09:35.9 XXXXX Time 25, Flow 0.61558, Mix 0 00:09:35.9 XXXXX Time 26, Flow 0.59126, Mix 0 00:09:36.0 XXXXX Time 27, Flow 0.56675, Mix 0 00:09:36.0 XXXXX Time 28, Flow 0.5421, Mix 0 00:09:36.0 XXXXX Time 29, Flow 0.51737, Mix 0 00:09:36.1 XXXXX Time 30, Flow 0.49262, Mix 0 00:09:36.1 XXXXX Time 31, Flow 0.46789, Mix 0 00:09:36.1 XXXXX Time 32, Flow 0.44324, Mix 0 00:09:36.2 XXXXX Time 33, Flow 0.41873, Mix 0 00:09:36.2 XXXXX Time 34, Flow 0.39441, Mix 0 00:09:36.3 XXXXX Time 35, Flow 0.37035, Mix 0 00:09:36.3 XXXXX Time 36, Flow 0.34658, Mix 0 00:09:36.3 XXXXX Time 37, Flow 0.32317, Mix 0 00:09:36.4 XXXXX Time 38, Flow 0.30017, Mix 0 00:09:36.4 XXXXX Time 39, Flow 0.27764, Mix 0 00:09:36.4 XXXXX Time 40, Flow 0.25564, Mix 0 00:09:36.5 XXXXX Time 41, Flow 0.23421, Mix 0 00:09:36.5 XXXXX Time 42, Flow 0.21341, Mix 0 00:09:36.6 XXXXX Time 43, Flow 0.19331, Mix 0 00:09:36.6 XXXXX Time 44, Flow 0.17394, Mix 0 00:09:36.6 XXXXX Time 45, Flow 0.15537, Mix 0 00:09:36.7 XXXXX Time 46, Flow 0.13766, Mix 0 00:09:36.7 XXXXX Time 47, Flow 0.12085, Mix 0 00:09:36.7 XXXXX Time 48, Flow 0.10501, Mix 0 00:09:36.8 XXXXX Time 49, Flow 0.09018, Mix 0 00:09:36.8 XXXXX Time 50, Flow 0.07643, Mix 0 00:09:36.9 XXXXX Time 51, Flow 0.0638, Mix 0 00:09:36.9 XXXXX Time 52, Flow 0.05236, Mix 0 00:09:36.9 XXXXX Time 53, Flow 0.04215, Mix 0 00:09:37.0 XXXXX Time 54, Flow 0.03324, Mix 0 00:09:37.0 XXXXX Time 55, Flow 0.02567, Mix 0 00:09:37.1 XXXXX Time 56, Flow 0.01951, Mix 0 00:09:37.1 XXXXX Time 57, Flow 0.01481, Mix 0 00:09:37.2 XXXXX Time 58, Flow 0.01162, Mix 0 00:09:37.2 XXXXX Time 59, Flow 0.00999, Mix 0 00:09:37.2 --- Gradient is created, switching valves A and B 00:09:37.2 pump: GetSetManualValvePosition(A) --> 240 00:09:37.2 pump: GetCurrentPressure(A) --> 91.44558 00:09:37.2 pump: SetManualValvePosition(A, 240, False) 00:09:37.4 pump: GetSetManualValvePosition(B) --> 240 00:09:37.4 pump: GetCurrentPressure(B) --> 13.75667 00:09:37.4 pump: SetManualValvePosition(B, 240, False) 00:09:37.6 --- Valves A and B switched to MixTee, starting gradient 00:09:37.6 --- LoadGradient() --- 00:09:37.6 --- Loading gradient method index: 0 00:09:37.6 pump: LoadMethod(0, ZrData.ZrPumpMethod) 00:09:38.1 pump: GetMethodState(0) --> loaded 00:09:38.1 --- Set gradient start 00:09:38.1 --- StartGradient() --- 00:09:38.1 --- Starting gradient method index: 0 00:09:38.1 pump: SetMethodSignal(0, StartTrunk) 00:09:38.3 pump: GetMethodState(0) --> runningTrunk 00:09:38.3 --- Start GradientSplineFit 00:09:38.3 pump: GetMethodState(0) --> runningTrunk 00:09:39.3 pump: GetCurrentPressure(A) --> 90.8737 00:09:39.3 pump: GetCurrentPressure(B) --> 14.22995 00:09:39.3 --- GradientSplineFit is running 00:10:37.4 --- GradientSplineFit has finished 00:10:37.4 --- End GradientSplineFit 00:10:37.4 --- Finished spline flow reduction A --- 00:10:37.4 --- Finished conditioning paths --- 00:10:42.6 autosampler-none: MoveValveDrive(Valve Drive 2, 240 deg) 00:10:42.7 pump: GetSetExternalTemperature() --> 50 00:10:42.7 --- Oven temperatur: 50 00:10:42.7 --- Separator: Name: IonOpticks 25 cm, Func: Separator, ColDia: 0.075 mm, Len: 250 mm, PartDia: 1.6 µm, Porosity: 42 % 00:10:42.7 --- Spline fitting time: 97.6577595902957 s 00:10:42.7 --- Spline fitting time adapted: 53.7117677746626 s 00:10:42.7 --- Press: 541.893115083548; colPress: 511.893115083548, visco: 0.00566684100509561, flow > 0.3 00:10:42.7 --- Flow: 0.3; Mix: 0, calcI: 220.674819441516 00:10:42.7 --- Ki calc: 220.674819441516 00:10:42.7 pump: GetSettings() --> PumpSettings 00:10:42.7 ------------------------------------------------------------------ 00:10:42.7 PID settings changed: 00:10:42.7 ------------------------------------------------------------------ 00:10:42.7 P: 200 : True; Pmin: -32000 : True; Pmax: 32000 : True 00:10:42.7 I: 600 : True; Imin: -32000 : True; Imax: 32000 : True; IWindow: 100 : True 00:10:42.7 D: 1000 : True; Dmin: -32000 : True; Dmax: 32000 : True 00:10:42.7 Result min: -32000 : True; Result max: 32000 : True 00:10:42.7 ------------------------------------------------------------------ 00:10:42.7 pump: SetSettings(PumpSettings) 00:10:42.7 pump: GetSettings() --> PumpSettings 00:10:42.7 ------------------------------------------------------------------ 00:10:42.7 PID settings changed: 00:10:42.7 ------------------------------------------------------------------ 00:10:42.7 P: 200 : False; Pmin: -32000 : False; Pmax: 32000 : False 00:10:42.7 I: 220 : True; Imin: -32000 : False; Imax: 32000 : False; IWindow: 100 : False 00:10:42.7 D: 1000 : False; Dmin: -32000 : False; Dmax: 32000 : False 00:10:42.7 Result min: -32000 : False; Result max: 32000 : False 00:10:42.7 ------------------------------------------------------------------ 00:10:42.7 pump: SetSettings(PumpSettings) 00:10:42.7 --- y = -5.33333333333333E-05x + 0.302864627614649 00:10:42.7 --- p0.Time -1, p0.Flow 0.01 00:10:42.7 --- p1.Time 0, p1.Flow 0.01 00:10:42.7 --- p2.Time 52.7117677746626, p2.Flow 0.300053333333333 00:10:42.7 --- p3.Time 53.7117677746626, p3.Flow 0.3, p3.Mix 0 00:10:42.7 --- p4.Time 1853.71176777466, p4.Flow 0.204, p4.Mix 32 00:10:42.7 --- Strategy function end 00:10:42.7 --- y = 5.33333333333333E-05x + -0.00286462761464867 00:10:42.7 --- p0.Time -1, p0.Flow 0 00:10:42.7 --- p1.Time 0, p1.Flow 0 00:10:42.7 --- p2.Time 52.7117677746626, p2.Flow -5.33333333333336E-05 00:10:42.7 --- p3.Time 53.7117677746626, p3.Flow 0, p3.Mix 0 00:10:42.7 --- p4.Time 1853.71176777466, p4.Flow 0.096, p4.Mix 32 00:10:42.7 --- Strategy function end 00:10:42.7 xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx 00:10:42.7 XXXXX Time 0, Flow 0.01, Mix 0 00:10:42.8 XXXXX Time 1, Flow 0.01059, Mix 0 00:10:42.8 XXXXX Time 2, Flow 0.01175, Mix 0 00:10:42.9 XXXXX Time 3, Flow 0.01347, Mix 0 00:10:42.9 XXXXX Time 4, Flow 0.01571, Mix 0 00:10:43.0 XXXXX Time 5, Flow 0.01845, Mix 0 00:10:43.0 XXXXX Time 6, Flow 0.02168, Mix 0 00:10:43.1 XXXXX Time 7, Flow 0.02537, Mix 0 00:10:43.1 XXXXX Time 8, Flow 0.02949, Mix 0 00:10:43.2 XXXXX Time 9, Flow 0.03402, Mix 0 00:10:43.2 XXXXX Time 10, Flow 0.03894, Mix 0 00:10:43.3 XXXXX Time 11, Flow 0.04424, Mix 0 00:10:43.3 XXXXX Time 12, Flow 0.04988, Mix 0 00:10:43.4 XXXXX Time 13, Flow 0.05584, Mix 0 00:10:43.4 XXXXX Time 14, Flow 0.0621, Mix 0 00:10:43.5 XXXXX Time 15, Flow 0.06864, Mix 0 00:10:43.5 XXXXX Time 16, Flow 0.07544, Mix 0 00:10:43.5 XXXXX Time 17, Flow 0.08247, Mix 0 00:10:43.6 XXXXX Time 18, Flow 0.08971, Mix 0 00:10:43.6 XXXXX Time 19, Flow 0.09713, Mix 0 00:10:43.7 XXXXX Time 20, Flow 0.10472, Mix 0 00:10:43.7 XXXXX Time 21, Flow 0.11246, Mix 0 00:10:43.8 XXXXX Time 22, Flow 0.12031, Mix 0 00:10:43.8 XXXXX Time 23, Flow 0.12826, Mix 0 00:10:43.9 XXXXX Time 24, Flow 0.13629, Mix 0 00:10:43.9 XXXXX Time 25, Flow 0.14437, Mix 0 00:10:44.0 XXXXX Time 26, Flow 0.15248, Mix 0 00:10:44.0 XXXXX Time 27, Flow 0.16059, Mix 0 00:10:44.0 XXXXX Time 28, Flow 0.16869, Mix 0 00:10:44.1 XXXXX Time 29, Flow 0.17675, Mix 0 00:10:44.1 XXXXX Time 30, Flow 0.18476, Mix 0 00:10:44.2 XXXXX Time 31, Flow 0.19267, Mix 0 00:10:44.2 XXXXX Time 32, Flow 0.20049, Mix 0 00:10:44.3 XXXXX Time 33, Flow 0.20817, Mix 0 00:10:44.3 XXXXX Time 34, Flow 0.2157, Mix 0 00:10:44.3 XXXXX Time 35, Flow 0.22306, Mix 0 00:10:44.4 XXXXX Time 36, Flow 0.23022, Mix 0 00:10:44.4 XXXXX Time 37, Flow 0.23717, Mix 0 00:10:44.5 XXXXX Time 38, Flow 0.24387, Mix 0 00:10:44.5 XXXXX Time 39, Flow 0.25031, Mix 0 00:10:44.6 XXXXX Time 40, Flow 0.25646, Mix 0 00:10:44.6 XXXXX Time 41, Flow 0.2623, Mix 0 00:10:44.6 XXXXX Time 42, Flow 0.26781, Mix 0 00:10:44.7 XXXXX Time 43, Flow 0.27297, Mix 0 00:10:44.7 XXXXX Time 44, Flow 0.27775, Mix 0 00:10:44.8 XXXXX Time 45, Flow 0.28213, Mix 0 00:10:44.8 XXXXX Time 46, Flow 0.28609, Mix 0 00:10:44.9 XXXXX Time 47, Flow 0.28961, Mix 0 00:10:44.9 XXXXX Time 48, Flow 0.29265, Mix 0 00:10:45.0 XXXXX Time 49, Flow 0.29521, Mix 0 00:10:45.0 XXXXX Time 50, Flow 0.29726, Mix 0 00:10:45.1 XXXXX Time 51, Flow 0.29877, Mix 0 00:10:45.1 XXXXX Time 52, Flow 0.29972, Mix 0 00:10:45.2 XXXXX Time 53, Flow 0.3001, Mix 0 00:10:45.2 --- Gradient is created, switching valves A and B 00:10:45.2 pump: GetSetManualValvePosition(A) --> 240 00:10:45.2 pump: GetCurrentPressure(A) --> 0.04338343 00:10:45.2 pump: SetManualValvePosition(A, 240, False) 00:10:45.4 pump: GetSetManualValvePosition(B) --> 240 00:10:45.4 pump: GetCurrentPressure(B) --> 0 00:10:45.4 pump: SetManualValvePosition(B, 240, False) 00:10:45.6 --- Valves A and B switched to MixTee, starting gradient 00:10:45.6 --- LoadGradient() --- 00:10:45.6 --- Loading gradient method index: 0 00:10:45.6 pump: LoadMethod(0, ZrData.ZrPumpMethod) 00:10:46.1 pump: GetMethodState(0) --> loaded 00:10:46.1 --- Set gradient start 00:10:46.1 --- StartGradient() --- 00:10:46.1 --- Starting gradient method index: 0 00:10:46.1 pump: SetMethodSignal(0, StartTrunk) 00:10:46.3 pump: GetMethodState(0) --> runningTrunk 00:10:46.3 --- Start GradientSplineFit 00:10:46.3 pump: GetMethodState(0) --> runningTrunk 00:10:47.3 pump: GetCurrentPressure(A) --> 0.9702234 00:10:47.3 pump: GetCurrentPressure(B) --> 0 00:10:47.3 --- GradientSplineFit is running 00:11:39.3 --- GradientSplineFit has finished 00:11:39.3 --- End GradientSplineFit 00:11:39.3 status: RemoveStatus(preparing gradient start) 00:11:39.6 Setting signal "lc-ready" 00:11:39.6 Waiting for signal "ms-start"... 00:11:39.6 --- LoadGradient() --- 00:11:39.6 --- Loading gradient method index: 0 00:11:39.6 pump: LoadMethod(0, ZrData.ZrPumpMethod) 00:11:40.1 pump: GetMethodState(0) --> loaded 00:11:40.1 --- Set gradient start 00:11:40.1 --- StartGradient() --- 00:11:40.1 --- Starting gradient method index: 0 00:11:40.1 pump: SetMethodSignal(0, StartTrunk) 00:11:40.3 pump: GetMethodState(0) --> runningTrunk 00:11:40.4 autosampler-none: SetSignal(Output Signal 1) 00:11:40.4 status: SetStatus(wash, ) 00:11:44.5 status: SetStatus(elution, ) 00:11:46.1 autosampler-left: MoveToObject(LCInjector1) 00:11:48.7 autosampler-left: PenetrateWithConstForce(LCInjector1) 00:11:48.9 autosampler-left: MoveValveDrive(Valve Drive 1, 0 deg) 00:12:18.5 autosampler-left: CleanInjector(LCInjector1, LCP 1, 100 uL, 0 mm, 5 uL/s, 5 s, 20 mm, 40 uL/s, 1000 ms, 2, False) 00:13:48.8 autosampler-left: CleanInjector(LCInjector1, LCP 1, 300 uL, 0 mm, 5 uL/s, 5 s, 20 mm, 40 uL/s, 1000 ms, 1, True) 00:13:49.0 autosampler-left: MoveValveDrive(Valve Drive 1, 60 deg) 00:13:52.5 autosampler-left: MoveToHome() 01:11:41.5 Setting signal "lc-done" 01:11:41.5 status: RemoveStatus(elution) 01:11:41.5 status: RemoveStatus(wash) 01:11:41.5 pump: GetCurrentPressure(A) --> 239.9766 01:11:41.5 pump: GetCurrentPressure(B) --> 248.1486 01:11:41.5 pump: GetSettings() --> PumpSettings 01:11:41.5 pump: GetSetManualValvePosition(A) --> 240 01:11:41.5 pump: GetSetManualValvePosition(B) --> 240 01:11:41.5 ------------------------------------------------------- 01:11:41.5 --- Reduce pressure --- 01:11:41.5 ------------------------------------------------------- 01:11:41.5 --- Pressure channel A: 239.976608276367 01:11:41.5 --- Pressure channel B: 248.148574829102 01:11:41.5 pump: GetSettings() --> PumpSettings 01:11:41.5 ------------------------------------------------------------------ 01:11:41.5 PID settings changed: 01:11:41.5 ------------------------------------------------------------------ 01:11:41.5 P: 250 : True; Pmin: -5500 : True; Pmax: 32000 : True 01:11:41.5 I: 50 : True; Imin: -20000 : True; Imax: 20000 : True; IWindow: 50 : True 01:11:41.5 D: 1500 : True; Dmin: -10000 : True; Dmax: 10000 : True 01:11:41.5 Result min: -32000 : True; Result max: 32000 : True 01:11:41.5 ------------------------------------------------------------------ 01:11:41.5 pump: SetSettings(PumpSettings) 01:11:41.7 --- Set pressure channel A: 10 01:11:41.7 --- Set pressure channel B: 5 01:11:41.7 --- Reduce pressure flow controlled: True 01:12:12.1 -------------------------------------------------------- 01:12:12.1 --- Log PID parameter called from: reducePressure 01:12:12.1 -------------------------------------------------------- 01:12:12.1 --- P: 200; Pmin: -32000; Pmax: 32000 01:12:12.1 --- I: 220; Imin: -32000; Imax: 32000; IWindow: 100 01:12:12.1 --- D: 1000; Dmin: -32000; Dmax: 32000 01:12:12.1 --- Result min: -32000; Result max: 32000 01:12:12.1 -------------------------------------------------------- 01:12:12.1 -------------------------------------------------------- 01:12:12.1 --- Log instrument settings called from: reducePressure 01:12:12.1 -------------------------------------------------------- 01:12:12.1 --- HomeSpeed: 2000; PurgeSpeed: 2000 01:12:12.1 --- PressureTrackingExcessFactor: 96 01:12:12.1 --- FlowResistanceA: 300; FlowResistanceB: 100; FlowResistanceOut: 150 01:12:12.1 --- FC_FlowFilterDepth: 5; FC_PressureFilterDepth: 0 01:12:12.1 --- PC_OutputFilterDepth: 0 01:12:12.1 --- CompressibilityFactorB: 2.29999995231628; PressurePIDNegativeResultDivisor: 1 01:12:12.1 --- ExternalZeroPressureOffsetA: 131.015731811523; ExternalZeroPressureOffsetB: 127.931526184082 01:12:12.1 --- ExternalPressureScalingFactorA: 0.0197199992835522; ExternalPressureScalingFactorB: 0.0197199992835522 01:12:12.1 -------------------------------------------------------- 01:12:12.1 --- Final pressure channel A: 4.97338342666626 01:12:12.1 --- Final pressure channel B: 3.69946908950806 01:12:12.1 ------------------------------------------------------- 01:12:12.7 autosampler-left: MoveValveDrive(Valve Drive 2, 0 deg) 01:12:12.8 pump: Manualmode_Pump_constantSpeed(A, 0) 01:12:12.8 pump: GetSetPistonSpeed(A) --> 0 01:12:12.8 pump: Manualmode_Pump_constantSpeed(B, 0) 01:12:12.8 pump: GetSetPistonSpeed(B) --> 0 01:12:12.8 pump: IsIdle() --> True 01:12:12.9 autosampler-left: MoveValveDrive(Valve Drive 1, 60 deg) 01:12:15.3 autosampler-left: MoveToHome() 01:12:15.4 pump: IsIdle() --> True 01:12:15.4 COMPLETED