Path: 2022-08-09_00-21-14_Two-column-separation Procedure: Two column separation Argument list: uses_trap = True uses_separator = True is_isocratic = False analysis_time = 1800 oven_temperature = 50 sample_volume = 5 sample_position = Slot2:40 presample_solvent_volume = 2 presample_air_volume = 0.5 postsample_air_volume = 1 postsample_solvent_volume = 2 sample_aspirate_speed = 5 sample_postaspirate_delay = 500 sample_inject_speed = 1 calibrant_volume = 0.6 calibrantTime = 10 is_calibrant_inject = False is_extended_wash = False is_bottom_sense = True injection_method = uLPickup trap = Name: Thermo Trap Cartridge 5mm, Func: Separator, ColDia: 0.3 mm, Len: 5 mm, PartDia: 5 µm, Porosity: 42 % trap_volume = 0.148440252882118 trap_unityflow = 2.78838695282287 trap_equilibration_volumemultiplier = 10 trap_equilibration_pressure = 217.5 trap_equil_time = 0.648440252882118 separator = Name: IonOpticks 25 cm, Func: Separator, ColDia: 0.075 mm, Len: 250 mm, PartDia: 1.6 µm, Porosity: 42 % separator_volume = 0.463875790256618 separator_unityflow = 0.00058605984562038 separator_equilibration_volumemultiplier = 4 separator_equilibration_pressure = 800 column_load_volumemultiplier = 4 column_load_pressure = 217.5 separator_equil_time = 4.05402026133927 column_load_time = 0.6 gradient = (0 sec,0.3 µL/min,0.00 %), (1800 sec,0.3 µL/min,32.00 %), (2700 sec,0.3 µL/min,40.00 %), (3000 sec,0.3 µL/min,60.00 %), (3300 sec,0.3 µL/min,95.00 %), (3600 sec,0.3 µL/min,95.00 %) 00:00:00.0 STARTED 00:00:00.9 Lua version: 1.1.0 00:00:00.9 Lua date: 2020/01/30 00:00:00.9 --- Experiment: Description 00:00:00.9 ------------------------------------------------------- 00:00:00.9 --- Trap Column: Thermo Trap Cartridge 5mm 00:00:00.9 --- Manufacturer: Thermo Scientific 00:00:00.9 --- Part Number: 00:00:00.9 --- Serial Number: 00:00:00.9 --- Type/Material: µ-Precolum TM Cartridge / PepMap TM C18 00:00:00.9 --- Length: 5 mm 00:00:00.9 --- Inner Diameter: 0.3 mm 00:00:00.9 --- Particle Diameter: 5 um 00:00:00.9 --- Is Particle Pore Size: True 00:00:00.9 --- Particle Pore Size: 100 00:00:00.9 --- Is Column Porosity: False 00:00:00.9 --- Column Porosity: 0.42 (0.0-1.0) 00:00:00.9 --- Is Max Temperature: False 00:00:00.9 --- max Temperature: 65 C 00:00:00.9 --- Is Max Pressure: False 00:00:00.9 --- max Pressure: 1000 bar 00:00:00.9 --- Is Max Flow: False 00:00:00.9 --- max Flow: 9 uL/min 00:00:00.9 --- Advanced Settings: False 00:00:00.9 --- Column Comments: Manufacturer no: 160454 00:00:00.9 ------------------------------------------------------- 00:00:00.9 --- Separation Column: IonOpticks 25 cm 00:00:00.9 --- Manufacturer: IonOpticks 00:00:00.9 --- Part Number: 00:00:00.9 --- Serial Number: 00:00:00.9 --- Type/Material: C18 00:00:00.9 --- Length: 250 mm 00:00:00.9 --- Inner Diameter: 0.075 mm 00:00:00.9 --- Particle Diameter: 1.6 um 00:00:00.9 --- Is Particle Pore Size: True 00:00:00.9 --- Particle Pore Size: 120 00:00:00.9 --- Is Column Porosity: False 00:00:00.9 --- Column Porosity: 0.42 (0.0-1.0) 00:00:00.9 --- Is Max Temperature: False 00:00:00.9 --- max Temperature: 65 C 00:00:00.9 --- Is Max Pressure: True 00:00:00.9 --- max Pressure: 1000 bar 00:00:00.9 --- Is Max Flow: False 00:00:00.9 --- max Flow: 9 uL/min 00:00:00.9 --- Advanced Settings: False 00:00:00.9 --- Column Comments: 00:00:00.9 ------------------------------------------------------- 00:00:00.9 'Valve Drive 1' is 'ILcInjectorValve' 00:00:00.9 'Valve Drive 2' is 'ISelectorValve' 00:00:00.9 --- Trap equil time: 0.64 00:00:00.9 --- Seperator equil time: 4.05 00:00:00.9 --- Column loading time: 0.6 00:00:00.9 pump: GetSettings() --> PumpSettings 00:00:00.9 -------------------------------------------------------- 00:00:00.9 --- Log instrument settings called from: Two Column Separation 00:00:00.9 -------------------------------------------------------- 00:00:00.9 --- HomeSpeed: 2000; PurgeSpeed: 2000 00:00:00.9 --- PressureTrackingExcessFactor: 96 00:00:00.9 --- FlowResistanceA: 300; FlowResistanceB: 100; FlowResistanceOut: 150 00:00:00.9 --- FC_FlowFilterDepth: 5; FC_PressureFilterDepth: 0 00:00:00.9 --- PC_OutputFilterDepth: 0 00:00:00.9 --- CompressibilityFactorB: 2.29999995231628; PressurePIDNegativeResultDivisor: 1 00:00:00.9 --- ExternalZeroPressureOffsetA: 130.944732666016; ExternalZeroPressureOffsetB: 128.061676025391 00:00:00.9 --- ExternalPressureScalingFactorA: 0.0197199992835522; ExternalPressureScalingFactorB: 0.0197199992835522 00:00:00.9 -------------------------------------------------------- 00:00:00.9 --- Oven temperature: 50 00:00:00.9 --- Separator: Name: IonOpticks 25 cm, Func: Separator, ColDia: 0.075 mm, Len: 250 mm, PartDia: 1.6 µm, Porosity: 42 % --- 00:00:00.9 --- Trap: Name: Thermo Trap Cartridge 5mm, Func: Separator, ColDia: 0.3 mm, Len: 5 mm, PartDia: 5 µm, Porosity: 42 % --- 00:00:00.9 --- MaxPressureLimitAB: 1000 bar --- 00:00:00.9 pump: GetCurrentPressure(A) --> 5.616262 00:00:00.9 pump: GetCurrentPressure(B) --> 6.487879 00:00:00.9 pump: GetSettings() --> PumpSettings 00:00:00.9 pump: GetSetManualValvePosition(A) --> 240 00:00:00.9 pump: GetSetManualValvePosition(B) --> 240 00:00:00.9 ------------------------------------------------------- 00:00:00.9 --- Reduce pressure --- 00:00:00.9 ------------------------------------------------------- 00:00:00.9 --- Pressure channel A: 5.61626243591309 00:00:00.9 --- Pressure channel B: 6.48787927627563 00:00:00.9 pump: Manualmode_Pump_constantSpeed(A, 0) 00:00:00.9 pump: GetSetPistonSpeed(A) --> 0 00:00:00.9 pump: Manualmode_Pump_constantSpeed(B, 0) 00:00:00.9 pump: GetSetPistonSpeed(B) --> 0 00:00:00.9 pump: IsIdle() --> True 00:00:00.9 ------------------------------------------------------- 00:00:01.0 pump: SetMaxPressureLimit(A, 1020) 00:00:01.0 pump: GetMaxPressureLimit(A) --> 1020 00:00:01.1 pump: SetMaxPressureLimit(B, 1020) 00:00:01.1 pump: GetMaxPressureLimit(B) --> 1020 00:00:01.1 pump: GetSettings() --> PumpSettings 00:00:01.1 ------------------------------------------------------------------ 00:00:01.1 PID settings changed: 00:00:01.1 ------------------------------------------------------------------ 00:00:01.1 P: 175 : True; Pmin: -5000 : True; Pmax: 5000 : True 00:00:01.1 I: 5 : True; Imin: -200 : True; Imax: 1000 : True; IWindow: 10 : True 00:00:01.1 D: 0 : True; Dmin: -1000 : True; Dmax: 1000 : True 00:00:01.1 Result min: -6000 : True; Result max: 8000 : True 00:00:01.1 ------------------------------------------------------------------ 00:00:01.1 pump: SetSettings(PumpSettings) 00:00:01.1 pump: GetPumpSideState(A) --> manualMode 00:00:01.1 pump: GetPumpSideState(B) --> manualMode 00:00:01.1 pump: GetPistonPosition(A) --> 391.695866444678 00:00:01.1 pump: GetPistonPosition(B) --> 73.2296869971371 00:00:01.1 ------------------------------------------------------- 00:00:01.1 --- Optional Refill of Pump Heads: 00:00:01.1 --- initiated by channel A: False, B: False 00:00:01.1 pump: GetPistonPosition(A) --> 391.695866444678 00:00:01.1 pump: GetPistonPosition(B) --> 73.2296869971371 00:00:01.1 --- Piston position channel A: 391.695866444678, B: 73.2296869971371 00:00:01.1 ------------------------------------------------------- 00:00:01.1 pump: SetDigitalOutput(PO2, False) 00:00:01.1 pump: GetCurrentPressure(A) --> 5.616262 00:00:01.1 pump: GetCurrentPressure(B) --> 6.428719 00:00:01.1 pump: GetSettings() --> PumpSettings 00:00:01.1 pump: GetSetManualValvePosition(A) --> 240 00:00:01.1 pump: GetSetManualValvePosition(B) --> 240 00:00:01.1 ------------------------------------------------------- 00:00:01.1 --- Reduce pressure --- 00:00:01.1 ------------------------------------------------------- 00:00:01.1 --- Pressure channel A: 5.61626243591309 00:00:01.1 --- Pressure channel B: 6.42871904373169 00:00:01.1 pump: GetSettings() --> PumpSettings 00:00:01.1 ------------------------------------------------------------------ 00:00:01.1 PID settings changed: 00:00:01.1 ------------------------------------------------------------------ 00:00:01.1 P: 250 : True; Pmin: -5500 : True; Pmax: 32000 : True 00:00:01.1 I: 50 : True; Imin: -20000 : True; Imax: 20000 : True; IWindow: 50 : True 00:00:01.1 D: 1500 : True; Dmin: -10000 : True; Dmax: 10000 : True 00:00:01.1 Result min: -32000 : True; Result max: 32000 : True 00:00:01.1 ------------------------------------------------------------------ 00:00:01.1 pump: SetSettings(PumpSettings) 00:00:01.4 --- Set pressure channel A: 10 00:00:01.4 --- Set pressure channel B: 5 00:00:01.4 --- Reduce pressure flow controlled: True 00:00:02.7 -------------------------------------------------------- 00:00:02.7 --- Log PID parameter called from: reducePressure 00:00:02.7 -------------------------------------------------------- 00:00:02.7 --- P: 175; Pmin: -5000; Pmax: 5000 00:00:02.7 --- I: 5; Imin: -200; Imax: 1000; IWindow: 10 00:00:02.7 --- D: 0; Dmin: -1000; Dmax: 1000 00:00:02.7 --- Result min: -6000; Result max: 8000 00:00:02.7 -------------------------------------------------------- 00:00:02.7 -------------------------------------------------------- 00:00:02.7 --- Log instrument settings called from: reducePressure 00:00:02.7 -------------------------------------------------------- 00:00:02.7 --- HomeSpeed: 2000; PurgeSpeed: 2000 00:00:02.7 --- PressureTrackingExcessFactor: 96 00:00:02.7 --- FlowResistanceA: 300; FlowResistanceB: 100; FlowResistanceOut: 150 00:00:02.7 --- FC_FlowFilterDepth: 5; FC_PressureFilterDepth: 0 00:00:02.7 --- PC_OutputFilterDepth: 0 00:00:02.7 --- CompressibilityFactorB: 2.29999995231628; PressurePIDNegativeResultDivisor: 1 00:00:02.7 --- ExternalZeroPressureOffsetA: 130.944732666016; ExternalZeroPressureOffsetB: 128.061676025391 00:00:02.7 --- ExternalPressureScalingFactorA: 0.0197199992835522; ExternalPressureScalingFactorB: 0.0197199992835522 00:00:02.7 -------------------------------------------------------- 00:00:02.7 --- Final pressure channel A: 5.79374217987061 00:00:02.7 --- Final pressure channel B: 0 00:00:02.7 ------------------------------------------------------- 00:00:02.7 pump: GetSetManualValvePosition(A) --> 240 00:00:02.7 pump: GetCurrentPressure(A) --> 5.793742 00:00:02.7 pump: SetManualValvePosition(A, 0, True) 00:00:03.3 pump: GetSetManualValvePosition(B) --> 240 00:00:03.3 pump: GetCurrentPressure(B) --> 0 00:00:03.3 pump: SetManualValvePosition(B, 180, False) 00:00:03.7 pump: GetSettings() --> PumpSettings 00:00:03.7 ------------------------------------------------------------------ 00:00:03.7 PID settings changed: 00:00:03.7 ------------------------------------------------------------------ 00:00:03.7 P: 250 : True; Pmin: -10000 : True; Pmax: 10000 : True 00:00:03.7 I: 20 : True; Imin: -10000 : True; Imax: 20000 : True; IWindow: 10 : True 00:00:03.7 D: 1500 : True; Dmin: -10000 : True; Dmax: 10000 : True 00:00:03.7 Result min: -10000 : True; Result max: 32000 : True 00:00:03.7 ------------------------------------------------------------------ 00:00:03.7 pump: SetSettings(PumpSettings) 00:00:03.7 status: SetStatus(equilibrate separation-column, this finishes at about: 4.85 min., ) 00:00:03.9 autosampler-none: MoveValveDrive(Valve Drive 2, 180 deg) 00:00:03.9 --- MaxPressureLimitA: 800 bar --- 00:00:03.9 pump: GetCurrentPressure(A) --> 5.892343 00:00:03.9 pump: GetCurrentPressure() --> 5.892343 00:00:03.9 pump: GetSettings() --> PumpSettings 00:00:03.9 pump: GetSetManualValvePosition(A) --> 0 00:00:03.9 ------------------------------------------------------- 00:00:03.9 --- Reduce pressure --- 00:00:03.9 ------------------------------------------------------- 00:00:03.9 --- Pressure channel A: 5.89234256744385 00:00:03.9 --- Pressure channel B: 5.89234256744385 00:00:03.9 pump: Manualmode_Pump_constantSpeed(A, 0) 00:00:03.9 pump: GetSetPistonSpeed(A) --> 0 00:00:03.9 pump: Manualmode_Pump_constantSpeed(, 0) 00:00:03.9 pump: GetSetPistonSpeed() --> 0 00:00:03.9 pump: IsIdle() --> True 00:00:03.9 ------------------------------------------------------- 00:00:04.0 pump: SetMaxPressureLimit(A, 820) 00:00:04.0 pump: GetMaxPressureLimit(A) --> 820 00:00:04.0 ------------------------------------------------------- 00:00:04.0 --- Separation Column Equilibration: 00:00:04.0 ------------------------------------------------------- 00:00:04.0 --- ovenTemp [C]: 50 00:00:04.0 --- Separator Unity-Flow @ ovenTemp [ul/min/bar]: 0.00058605984562038 00:00:04.0 --- Separator Volume [ul]: 0.463875790256618 00:00:04.0 --- Separator Equilibration Volume Multiplier: 4 00:00:04.0 --- Separator Equilibration Pressure [bar]: 800 00:00:04.0 --- Separator Equilibration Flow [ul/min]: 0.457694594849779 00:00:04.0 --- Separator Equilibration Volume [ul]: 1.85550316102647 00:00:04.0 --- Separator Equilibration Time [min]: 4.05402026133927 00:00:04.0 --- max. Volume (10*V+2ul/min*t) [ul]: 26.6630721329433 00:00:04.0 --- Separator Equilibration Time Out [min]: 40.5402026133927 00:00:04.0 ------------------------------------------------------- 00:00:04.0 pump: Manualmode_Pump_constantPressure(A, 800) 00:00:04.0 pump: GetPistonPosition(A) --> 391.696075884188 00:00:04.0 --- Injection delay: 237.164855895902s 00:00:27.1 pump: GetPistonPosition(A) --> 468.709078194288 00:00:27.1 pump: GetPistonPosition(A) --> 468.709078194288 00:00:27.1 pump: GetPistonPosition(A) --> 468.709078194288 00:04:01.6 status: SetStatus(aspirate & inject, ) 00:04:01.7 autosampler-left: LeaveObject() 00:04:06.1 autosampler-left: MoveToObject(LCMS Wash 1, 3) 00:04:06.8 autosampler-left: PenetrateObject(LCMS Wash 1, 3) 00:04:06.9 autosampler-left: EmptySyringe() 00:04:07.6 autosampler-left: LeaveObject() 00:04:07.6 Cleaning syringe and needle exterior 00:04:23.4 autosampler-left: CleanSyringe(LCMS Wash 1, 1, 30 mm, 40 uL/s, 500 ms, 30 mm, 40 uL/s, 80 %, 2) 00:04:23.5 autosampler-left: ChangeTool(LCP 1) --> False 00:04:26.6 autosampler-left: MoveToObject(Item Position 3, 1, True, True, True) 00:04:29.8 autosampler-left: PenetrateWithBottomSense(Item Position 3, 1, 1 mm) 00:04:30.9 autosampler-left: AspirateSyringe(2 uL, 5 uL/s, 500 ms) 00:04:31.1 pump: GetPistonPosition(A) --> 473.60284179054 00:04:31.1 pump: GetCurrentPressure(A) --> 799.9378 00:04:31.1 --- act. pressure [bar]: 799.937805175781 00:04:31.1 --- used volume [uL]: 4.89376359625192 00:04:31.1 --- average flow (incl. leaks) [uL/min]: 1.20300978291644 00:04:31.1 --- act. equilibration time [min]: 4.06793333333335 00:04:31.1 ------------------------------------------------------- 00:04:31.1 status: RemoveStatus(equilibrate separation-column, this finishes at about: 4.85 min.) 00:04:31.1 status: SetStatus(equilibrate trap-column, this finishes at about: 5.65 min., ) 00:04:31.2 --- MaxPressureLimitA: 217.5 bar --- 00:04:31.2 pump: GetCurrentPressure(A) --> 799.9378 00:04:31.2 pump: GetCurrentPressure() --> 799.9378 00:04:31.2 pump: GetSettings() --> PumpSettings 00:04:31.2 pump: GetSetManualValvePosition(A) --> 0 00:04:31.2 ------------------------------------------------------- 00:04:31.2 --- Reduce pressure --- 00:04:31.2 ------------------------------------------------------- 00:04:31.2 --- Pressure channel A: 799.937805175781 00:04:31.2 --- Pressure channel B: 799.937805175781 00:04:31.2 pump: GetSettings() --> PumpSettings 00:04:31.2 ------------------------------------------------------------------ 00:04:31.2 PID settings changed: 00:04:31.2 ------------------------------------------------------------------ 00:04:31.2 P: 250 : True; Pmin: -32000 : True; Pmax: 32000 : True 00:04:31.2 I: 20 : True; Imin: -20000 : True; Imax: 20000 : True; IWindow: 100 : True 00:04:31.2 D: 1500 : True; Dmin: -10000 : True; Dmax: 10000 : True 00:04:31.2 Result min: -32000 : True; Result max: 32000 : True 00:04:31.2 ------------------------------------------------------------------ 00:04:31.2 pump: SetSettings(PumpSettings) 00:04:31.3 --- Set pressure channel A: 217.5 00:04:31.3 --- Set pressure channel B: 217.5 00:04:31.3 --- Reduce pressure flow controlled: False 00:04:31.7 autosampler-left: LeaveObject() 00:04:32.7 autosampler-left: AspirateSyringe(0.5 uL, 5 uL/s, 500 ms) 00:04:42.6 autosampler-left: MoveToObject(Rack 2, 40, True, True, True) 00:04:45.9 -------------------------------------------------------- 00:04:45.9 --- Log PID parameter called from: reducePressure 00:04:45.9 -------------------------------------------------------- 00:04:45.9 --- P: 250; Pmin: -10000; Pmax: 10000 00:04:45.9 --- I: 20; Imin: -10000; Imax: 20000; IWindow: 10 00:04:45.9 --- D: 1500; Dmin: -10000; Dmax: 10000 00:04:45.9 --- Result min: -10000; Result max: 32000 00:04:45.9 -------------------------------------------------------- 00:04:45.9 -------------------------------------------------------- 00:04:45.9 --- Log instrument settings called from: reducePressure 00:04:45.9 -------------------------------------------------------- 00:04:45.9 --- HomeSpeed: 2000; PurgeSpeed: 2000 00:04:45.9 --- PressureTrackingExcessFactor: 96 00:04:45.9 --- FlowResistanceA: 300; FlowResistanceB: 100; FlowResistanceOut: 150 00:04:45.9 --- FC_FlowFilterDepth: 5; FC_PressureFilterDepth: 0 00:04:45.9 --- PC_OutputFilterDepth: 0 00:04:45.9 --- CompressibilityFactorB: 2.29999995231628; PressurePIDNegativeResultDivisor: 1 00:04:45.9 --- ExternalZeroPressureOffsetA: 130.944732666016; ExternalZeroPressureOffsetB: 128.061676025391 00:04:45.9 --- ExternalPressureScalingFactorA: 0.0197199992835522; ExternalPressureScalingFactorB: 0.0197199992835522 00:04:45.9 -------------------------------------------------------- 00:04:45.9 --- Final pressure channel A: 216.127258300781 00:04:45.9 --- Final pressure channel B: 216.127258300781 00:04:45.9 ------------------------------------------------------- 00:04:46.0 pump: SetMaxPressureLimit(A, 237.5) 00:04:46.0 pump: GetMaxPressureLimit(A) --> 237.5 00:04:46.5 autosampler-none: MoveValveDrive(Valve Drive 2, 60 deg) 00:04:46.5 ------------------------------------------------------- 00:04:46.5 --- Trap Column Equilibration 00:04:46.5 ------------------------------------------------------- 00:04:46.5 --- Trap Temperature: fixed to 20 C 00:04:46.5 --- Trap Unity-Flow @ 20C [ul/bar]: 2.78838695282287 00:04:46.5 --- Trap Volume [ul]: 0.148440252882118 00:04:46.5 --- Trap Equilibration Volume Multiplier: 10 00:04:46.5 --- Trap Equilibration Pressure [bar]: 217.5 00:04:46.5 --- Trap Equilibration Flow [ul/min]: 9.99557465783688 00:04:46.5 --- Trap Equilibration Volume (+5) [ul]: 6.48440252882118 00:04:46.5 --- Trap Equilibration Time [min]: 0.648727336925764 00:04:46.5 --- max. Volume (10*V+2ul/min*t) [ul]: 66.1414799620633 00:04:46.5 ------------------------------------------------------- 00:04:46.5 pump: Manualmode_Pump_constantPressure(A, 217.5) 00:04:46.5 pump: GetPistonPosition(A) --> 419.453513055225 00:04:46.5 autosampler-left: PenetrateWithBottomSense(Rack 2, 40, 1 mm) 00:04:48.2 autosampler-left: AspirateSyringe(5 uL, 5 uL/s, 500 ms) 00:04:55.7 autosampler-left: LeaveObject() 00:04:56.6 autosampler-left: AspirateSyringe(1 uL, 5 uL/s, 500 ms) 00:05:00.0 autosampler-left: MoveToObject(LCMS Wash 1, 1, True, True, True) 00:05:01.0 autosampler-left: PenetrateObject(LCMS Wash 1, 1, 30 mm) 00:05:06.0 autosampler-left: Wait(5 s) 00:05:06.8 autosampler-left: LeaveObject() 00:05:07.5 pump: GetPistonPosition(A) --> 422.046374191988 00:05:07.5 pump: GetPistonPosition(A) --> 422.046374191988 00:05:07.5 pump: GetPistonPosition(A) --> 422.046374191988 00:05:10.1 autosampler-left: MoveToObject(LCInjector1) 00:05:10.9 autosampler-left: DispenseSyringe(0.75 uL, 1 uL/s) 00:05:13.6 autosampler-left: PenetrateWithConstForce(LCInjector1) 00:05:13.7 autosampler-left: MoveValveDrive(Valve Drive 1, 0 deg) 00:05:14.8 autosampler-left: Wait(1000 ms) 00:05:21.6 autosampler-left: DispenseSyringe(6.75 uL, 1 uL/s) 00:05:22.7 autosampler-left: Wait(1000 ms) 00:05:23.9 status: RemoveStatus(aspirate & inject) 00:05:46.9 pump: GetPistonPosition(A) --> 426.713105359141 00:05:46.9 pump: GetPistonPosition(A) --> 426.713105359141 00:06:02.9 pump: GetPistonPosition(A) --> 428.612512277501 00:06:02.9 pump: GetCurrentPressure(A) --> 217.4682 00:06:02.9 ------------------------------------------------------- 00:06:02.9 --- Finished: Trap Column Equilibration (const_pressure_load_and_equilibration) 00:06:02.9 ------------------------------------------------------- 00:06:02.9 --- act. pressure [bar]: 217.468215942383 00:06:02.9 --- used volume [uL]: 6.56613808551293 00:06:02.9 --- average flow [uL/min]: 7.11776486233267 00:06:02.9 --- act. equilibration time [min]: 0.922500000001552 00:06:02.9 ------------------------------------------------------- 00:06:02.9 status: RemoveStatus(equilibrate trap-column, this finishes at about: 5.65 min.) 00:06:04.9 --- MaxPressureLimitA: 217.5 bar --- 00:06:04.9 pump: GetCurrentPressure(A) --> 217.4091 00:06:04.9 pump: GetCurrentPressure() --> 217.4091 00:06:04.9 pump: GetSettings() --> PumpSettings 00:06:04.9 pump: GetSetManualValvePosition(A) --> 0 00:06:04.9 ------------------------------------------------------- 00:06:04.9 --- Reduce pressure --- 00:06:04.9 ------------------------------------------------------- 00:06:04.9 --- Pressure channel A: 217.409057617188 00:06:04.9 --- Pressure channel B: 217.409057617188 00:06:04.9 pump: Manualmode_Pump_constantSpeed(A, 0) 00:06:04.9 pump: GetSetPistonSpeed(A) --> 0 00:06:04.9 pump: Manualmode_Pump_constantSpeed(, 0) 00:06:04.9 pump: GetSetPistonSpeed() --> 0 00:06:04.9 pump: IsIdle() --> False 00:06:04.9 ------------------------------------------------------- 00:06:05.0 pump: SetMaxPressureLimit(A, 237.5) 00:06:05.0 pump: GetMaxPressureLimit(A) --> 237.5 00:06:05.0 status: SetStatus(load trap-column, this finishes at about: 8.35 min., ) 00:06:05.1 autosampler-left: MoveValveDrive(Valve Drive 1, 60 deg) 00:06:06.5 ------------------------------------------------------- 00:06:06.5 --- Sample Loading on Trap Column 00:06:06.5 ------------------------------------------------------- 00:06:06.5 --- Trap Temperature: fixed to 20 C 00:06:06.5 --- Trap Unity-Flow [ul/bar]: 2.78838695282287 00:06:06.5 --- Trap Volume [ul]: 0.148440252882118 00:06:06.5 --- Sample Volume [ul]: 5 00:06:06.5 --- Sample Volume Multiplier: 4 00:06:06.5 --- Trap Loading Pressure [bar]: 217.5 00:06:06.5 --- Trap Loading Flow [uL/min]: 9.99557465783688 00:06:06.5 --- Trap Loading Volume (+2) [uL]: 22 00:06:06.5 --- Trap Loading Time [min]: 2.200974006307 00:06:06.5 --- max. Volume (10*V+2ul/min*t) [ul]: 224.401948012614 00:06:06.5 ------------------------------------------------------- 00:06:06.5 pump: Manualmode_Pump_constantPressure(A, 217.5) 00:06:06.5 pump: GetPistonPosition(A) --> 428.86530576636 00:06:06.6 autosampler-left: EmptySyringe(5 uL/s) 00:06:22.1 pump: GetPistonPosition(A) --> 431.446228851039 00:06:22.1 pump: GetPistonPosition(A) --> 431.446228851039 00:06:22.1 pump: GetPistonPosition(A) --> 431.446228851039 00:07:06.0 autosampler-left: CleanInjector(LCInjector1, LCP 1, 200 uL, 0 mm, 5 uL/s, 5 s, 20 mm, 40 uL/s, 1000 ms, 2, False) 00:08:07.2 autosampler-left: CleanInjector(LCInjector1, LCP 1, 200 uL, 0 mm, 5 uL/s, 5 s, 20 mm, 40 uL/s, 1000 ms, 1, True) 00:08:22.9 autosampler-left: CleanSyringe(LCMS Wash 1, 2, 30 mm, 40 uL/s, 500 ms, 30 mm, 40 uL/s, 80 %, 2) 00:08:34.2 pump: GetPistonPosition(A) --> 447.303941368809 00:08:34.2 pump: GetPistonPosition(A) --> 447.303941368809 00:08:43.3 autosampler-left: CleanSyringe(LCMS Wash 1, 1, 30 mm, 40 uL/s, 500 ms, 30 mm, 40 uL/s, 80 %, 3) 00:08:46.6 autosampler-left: MoveToHome() 00:09:25.2 pump: GetPistonPosition(A) --> 453.468793352704 00:09:25.2 pump: GetCurrentPressure(A) --> 217.4288 00:09:25.2 ------------------------------------------------------- 00:09:25.2 --- Finished: Sample Loading on Trap Column (const_pressure_load_and_equilibration) 00:09:25.2 ------------------------------------------------------- 00:09:25.2 --- act. pressure [bar]: 217.428771972656 00:09:25.2 --- used volume [uL]: 22.0225645016645 00:09:25.2 --- average flow [uL/min]: 7.21487509814507 00:09:25.2 --- act. loading time [min]: 3.052383333334 00:09:25.2 ------------------------------------------------------- 00:09:25.2 --- Separator: Name: IonOpticks 25 cm, Func: Separator, ColDia: 0.075 mm, Len: 250 mm, PartDia: 1.6 µm, Porosity: 42 % --- 00:09:25.2 --- Trap: Name: Thermo Trap Cartridge 5mm, Func: Separator, ColDia: 0.3 mm, Len: 5 mm, PartDia: 5 µm, Porosity: 42 % --- 00:09:25.2 --- MaxPressureLimitAB: 1000 bar --- 00:09:25.2 pump: GetCurrentPressure(A) --> 217.4288 00:09:25.2 pump: GetCurrentPressure(B) --> 0 00:09:25.2 pump: GetSettings() --> PumpSettings 00:09:25.2 pump: GetSetManualValvePosition(A) --> 0 00:09:25.2 ------------------------------------------------------- 00:09:25.2 --- Reduce pressure --- 00:09:25.2 ------------------------------------------------------- 00:09:25.2 --- Pressure channel A: 217.428771972656 00:09:25.2 --- Pressure channel B: 0 00:09:25.2 pump: Manualmode_Pump_constantSpeed(A, 0) 00:09:25.2 pump: GetSetPistonSpeed(A) --> 0 00:09:25.2 pump: Manualmode_Pump_constantSpeed(B, 0) 00:09:25.2 pump: GetSetPistonSpeed(B) --> 0 00:09:25.2 pump: IsIdle() --> False 00:09:25.2 ------------------------------------------------------- 00:09:25.3 pump: SetMaxPressureLimit(A, 1020) 00:09:25.3 pump: GetMaxPressureLimit(A) --> 1020 00:09:25.4 pump: SetMaxPressureLimit(B, 1020) 00:09:25.4 pump: GetMaxPressureLimit(B) --> 1020 00:09:25.4 status: RemoveStatus(load trap-column, this finishes at about: 8.35 min.) 00:09:25.4 pump: GetSettings() --> PumpSettings 00:09:25.4 ------------------------------------------------------------------ 00:09:25.4 PID settings changed: 00:09:25.4 ------------------------------------------------------------------ 00:09:25.4 P: 100 : True; Pmin: -10000 : True; Pmax: 10000 : True 00:09:25.4 I: 100 : True; Imin: -1000 : True; Imax: 1000 : True; IWindow: 100 : True 00:09:25.4 D: 5000 : True; Dmin: -32000 : True; Dmax: 32000 : True 00:09:25.4 Result min: -5000 : True; Result max: 25000 : True 00:09:25.4 ------------------------------------------------------------------ 00:09:25.4 pump: SetSettings(PumpSettings) 00:09:25.4 pump: GetSettings() --> PumpSettings 00:09:25.4 ------------------------------------------------------------------ 00:09:25.4 PID settings changed: 00:09:25.4 ------------------------------------------------------------------ 00:09:25.4 P: 100 : False; Pmin: -10000 : False; Pmax: 10000 : False 00:09:25.4 I: 100 : False; Imin: -1000 : False; Imax: 1000 : False; IWindow: 2 : True 00:09:25.4 D: 5000 : False; Dmin: -32000 : False; Dmax: 32000 : False 00:09:25.4 Result min: -5000 : False; Result max: 25000 : False 00:09:25.4 ------------------------------------------------------------------ 00:09:25.4 pump: SetSettings(PumpSettings) 00:09:25.4 status: SetStatus(preparing gradient start, ) 00:09:25.5 --- Starting conditioning paths --- 00:09:25.5 pump: GetSettings() --> PumpSettings 00:09:25.5 ------------------------------------------------------------------ 00:09:25.5 PID settings changed: 00:09:25.5 ------------------------------------------------------------------ 00:09:25.5 P: 100 : True; Pmin: -10000 : True; Pmax: 10000 : True 00:09:25.5 I: 100 : True; Imin: -1000 : True; Imax: 1000 : True; IWindow: 100 : True 00:09:25.5 D: 5000 : True; Dmin: -32000 : True; Dmax: 32000 : True 00:09:25.5 Result min: -5000 : True; Result max: 25000 : True 00:09:25.5 ------------------------------------------------------------------ 00:09:25.5 pump: SetSettings(PumpSettings) 00:09:25.5 pump: Manualmode_Pump_constantPressure(A, 0) 00:09:25.5 pump: GetTargetPressure(A) --> 0 00:09:25.5 pump: GetCurrentPressure(A) --> 217.4485 00:09:26.5 pump: GetCurrentPressure(A) --> 202.5993 00:09:27.6 pump: GetCurrentPressure(A) --> 181.2623 00:09:28.6 pump: GetCurrentPressure(A) --> 160.1816 00:09:29.6 pump: GetCurrentPressure(A) --> 138.4305 00:09:30.6 pump: GetCurrentPressure(A) --> 117.2315 00:09:31.6 pump: GetCurrentPressure(A) --> 96.20994 00:09:32.6 pump: GetCurrentPressure(A) --> 75.3659 00:09:33.6 pump: GetCurrentPressure(A) --> 57.44042 00:09:34.6 pump: GetCurrentPressure(A) --> 37.66126 00:09:35.6 pump: GetCurrentPressure(A) --> 22.16134 00:09:36.6 pump: GetCurrentPressure(A) --> 14.05642 00:09:36.7 autosampler-none: MoveValveDrive(Valve Drive 2, 0 deg) 00:09:36.7 pump: SetManualValvePosition(A, 240, False) 00:09:37.3 pump: SetManualValvePosition(B, 240, False) 00:09:39.3 --- Set constant binary flow --- 00:09:39.3 pump: Manualmode_Pump_constantFlow_binary(1, 1) 00:09:39.3 pump: GetSetFlow(A) --> 1 00:09:39.3 pump: GetSetFlow(B) --> 1 00:09:39.3 00:09:39.3 --- Starting spline flow reduction B/A --- 00:09:39.4 pump: GetSetExternalTemperature() --> 50 00:09:39.4 --- Oven temperatur: 50 00:09:39.4 --- Separator: Name: IonOpticks 25 cm, Func: Separator, ColDia: 0.075 mm, Len: 250 mm, PartDia: 1.6 µm, Porosity: 42 % 00:09:39.4 --- Spline fitting time: 97.6577595902957 s 00:09:39.4 --- Spline fitting time adapted: 10 s 00:09:39.4 --- y = 0x + 1 00:09:39.4 --- p0.Time -1, p0.Flow 1 00:09:39.4 --- p1.Time 0, p1.Flow 1 00:09:39.4 --- p2.Time 9, p2.Flow 1 00:09:39.4 --- p3.Time 10, p3.Flow 1, p3.Mix 0 00:09:39.4 --- p4.Time 40, p4.Flow 1, p4.Mix 0 00:09:39.4 --- Strategy function end 00:09:39.4 --- y = 0x + 0 00:09:39.4 --- p0.Time -1, p0.Flow 1 00:09:39.4 --- p1.Time 0, p1.Flow 1 00:09:39.4 --- p2.Time 9, p2.Flow 0 00:09:39.4 --- p3.Time 10, p3.Flow 0, p3.Mix 0 00:09:39.4 --- p4.Time 40, p4.Flow 0, p4.Mix 0 00:09:39.4 --- Strategy function end 00:09:39.4 xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx 00:09:39.4 XXXXX Time 0, Flow 2, Mix 50 00:09:39.4 XXXXX Time 1, Flow 1.94545, Mix 48.59813 00:09:39.5 XXXXX Time 2, Flow 1.84848, Mix 45.90163 00:09:39.5 XXXXX Time 3, Flow 1.72121, Mix 41.9014 00:09:39.5 XXXXX Time 4, Flow 1.57575, Mix 36.53846 00:09:39.5 XXXXX Time 5, Flow 1.42424, Mix 29.78723 00:09:39.5 XXXXX Time 6, Flow 1.27878, Mix 21.80094 00:09:39.6 XXXXX Time 7, Flow 1.15151, Mix 13.15789 00:09:39.6 XXXXX Time 8, Flow 1.05454, Mix 5.17241 00:09:39.6 XXXXX Time 9, Flow 1, Mix 0 00:09:39.6 --- Gradient is created, switching valves A and B 00:09:39.6 pump: GetSetManualValvePosition(A) --> 240 00:09:39.6 pump: GetCurrentPressure(A) --> 4.709142 00:09:39.6 pump: SetManualValvePosition(A, 240, False) 00:09:39.8 pump: GetSetManualValvePosition(B) --> 240 00:09:39.8 pump: GetCurrentPressure(B) --> 0.3943993 00:09:39.8 pump: SetManualValvePosition(B, 240, False) 00:09:40.0 --- Valves A and B switched to MixTee, starting gradient 00:09:40.0 --- LoadGradient() --- 00:09:40.0 --- Loading gradient method index: 0 00:09:40.0 pump: LoadMethod(0, ZrData.ZrPumpMethod) 00:09:40.5 pump: GetMethodState(0) --> loaded 00:09:40.5 --- Set gradient start 00:09:40.5 --- StartGradient() --- 00:09:40.5 --- Starting gradient method index: 0 00:09:40.5 pump: SetMethodSignal(0, StartTrunk) 00:09:40.7 pump: GetMethodState(0) --> runningTrunk 00:09:40.7 --- Start GradientSplineFit 00:09:40.7 pump: GetMethodState(0) --> runningTrunk 00:09:41.7 pump: GetCurrentPressure(A) --> 79.72402 00:09:41.7 pump: GetCurrentPressure(B) --> 46.2434 00:09:41.7 --- GradientSplineFit is running 00:09:49.7 --- GradientSplineFit has finished 00:09:49.7 --- End GradientSplineFit 00:09:49.7 --- Finished spline flow reduction B --- 00:09:50.8 pump: GetSetExternalTemperature() --> 50 00:09:50.8 --- Oven temperatur: 50 00:09:50.8 --- Separator: Name: IonOpticks 25 cm, Func: Separator, ColDia: 0.075 mm, Len: 250 mm, PartDia: 1.6 µm, Porosity: 42 % 00:09:50.8 --- Spline fitting time: 97.6577595902957 s 00:09:50.8 --- Spline fitting time adapted: 60 s 00:09:50.8 --- y = 0x + 0.01 00:09:50.8 --- p0.Time -1, p0.Flow 1 00:09:50.8 --- p1.Time 0, p1.Flow 1 00:09:50.8 --- p2.Time 59, p2.Flow 0.01 00:09:50.8 --- p3.Time 60, p3.Flow 0.01, p3.Mix 0 00:09:50.8 --- p4.Time 90, p4.Flow 0.01, p4.Mix 0 00:09:50.8 --- Strategy function end 00:09:50.8 --- y = 0x + 0 00:09:50.8 --- p0.Time -1, p0.Flow 0 00:09:50.8 --- p1.Time 0, p1.Flow 0 00:09:50.8 --- p2.Time 59, p2.Flow 0 00:09:50.8 --- p3.Time 60, p3.Flow 0, p3.Mix 0 00:09:50.8 --- p4.Time 90, p4.Flow 0, p4.Mix 0 00:09:50.8 --- Strategy function end 00:09:50.8 xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx 00:09:50.8 XXXXX Time 0, Flow 1, Mix 0 00:09:50.8 XXXXX Time 1, Flow 0.99837, Mix 0 00:09:50.9 XXXXX Time 2, Flow 0.99518, Mix 0 00:09:50.9 XXXXX Time 3, Flow 0.99048, Mix 0 00:09:50.9 XXXXX Time 4, Flow 0.98432, Mix 0 00:09:50.9 XXXXX Time 5, Flow 0.97675, Mix 0 00:09:50.9 XXXXX Time 6, Flow 0.96784, Mix 0 00:09:51.0 XXXXX Time 7, Flow 0.95763, Mix 0 00:09:51.0 XXXXX Time 8, Flow 0.94619, Mix 0 00:09:51.0 XXXXX Time 9, Flow 0.93356, Mix 0 00:09:51.0 XXXXX Time 10, Flow 0.91981, Mix 0 00:09:51.0 XXXXX Time 11, Flow 0.90498, Mix 0 00:09:51.1 XXXXX Time 12, Flow 0.88914, Mix 0 00:09:51.1 XXXXX Time 13, Flow 0.87233, Mix 0 00:09:51.1 XXXXX Time 14, Flow 0.85462, Mix 0 00:09:51.1 XXXXX Time 15, Flow 0.83605, Mix 0 00:09:51.1 XXXXX Time 16, Flow 0.81668, Mix 0 00:09:51.2 XXXXX Time 17, Flow 0.79658, Mix 0 00:09:51.2 XXXXX Time 18, Flow 0.77578, Mix 0 00:09:51.2 XXXXX Time 19, Flow 0.75435, Mix 0 00:09:51.2 XXXXX Time 20, Flow 0.73235, Mix 0 00:09:51.2 XXXXX Time 21, Flow 0.70982, Mix 0 00:09:51.3 XXXXX Time 22, Flow 0.68682, Mix 0 00:09:51.3 XXXXX Time 23, Flow 0.66341, Mix 0 00:09:51.3 XXXXX Time 24, Flow 0.63964, Mix 0 00:09:51.3 XXXXX Time 25, Flow 0.61558, Mix 0 00:09:51.3 XXXXX Time 26, Flow 0.59126, Mix 0 00:09:51.4 XXXXX Time 27, Flow 0.56675, Mix 0 00:09:51.4 XXXXX Time 28, Flow 0.5421, Mix 0 00:09:51.4 XXXXX Time 29, Flow 0.51737, Mix 0 00:09:51.4 XXXXX Time 30, Flow 0.49262, Mix 0 00:09:51.4 XXXXX Time 31, Flow 0.46789, Mix 0 00:09:51.5 XXXXX Time 32, Flow 0.44324, Mix 0 00:09:51.5 XXXXX Time 33, Flow 0.41873, Mix 0 00:09:51.5 XXXXX Time 34, Flow 0.39441, Mix 0 00:09:51.5 XXXXX Time 35, Flow 0.37035, Mix 0 00:09:51.5 XXXXX Time 36, Flow 0.34658, Mix 0 00:09:51.6 XXXXX Time 37, Flow 0.32317, Mix 0 00:09:51.6 XXXXX Time 38, Flow 0.30017, Mix 0 00:09:51.6 XXXXX Time 39, Flow 0.27764, Mix 0 00:09:51.6 XXXXX Time 40, Flow 0.25564, Mix 0 00:09:51.6 XXXXX Time 41, Flow 0.23421, Mix 0 00:09:51.6 XXXXX Time 42, Flow 0.21341, Mix 0 00:09:51.7 XXXXX Time 43, Flow 0.19331, Mix 0 00:09:51.7 XXXXX Time 44, Flow 0.17394, Mix 0 00:09:51.7 XXXXX Time 45, Flow 0.15537, Mix 0 00:09:51.7 XXXXX Time 46, Flow 0.13766, Mix 0 00:09:51.7 XXXXX Time 47, Flow 0.12085, Mix 0 00:09:51.8 XXXXX Time 48, Flow 0.10501, Mix 0 00:09:51.8 XXXXX Time 49, Flow 0.09018, Mix 0 00:09:51.8 XXXXX Time 50, Flow 0.07643, Mix 0 00:09:51.8 XXXXX Time 51, Flow 0.0638, Mix 0 00:09:51.8 XXXXX Time 52, Flow 0.05236, Mix 0 00:09:51.9 XXXXX Time 53, Flow 0.04215, Mix 0 00:09:51.9 XXXXX Time 54, Flow 0.03324, Mix 0 00:09:51.9 XXXXX Time 55, Flow 0.02567, Mix 0 00:09:51.9 XXXXX Time 56, Flow 0.01951, Mix 0 00:09:51.9 XXXXX Time 57, Flow 0.01481, Mix 0 00:09:52.0 XXXXX Time 58, Flow 0.01162, Mix 0 00:09:52.0 XXXXX Time 59, Flow 0.00999, Mix 0 00:09:52.0 --- Gradient is created, switching valves A and B 00:09:52.0 pump: GetSetManualValvePosition(A) --> 240 00:09:52.0 pump: GetCurrentPressure(A) --> 90.8461 00:09:52.0 pump: SetManualValvePosition(A, 240, False) 00:09:52.2 pump: GetSetManualValvePosition(B) --> 240 00:09:52.2 pump: GetCurrentPressure(B) --> 12.85744 00:09:52.2 pump: SetManualValvePosition(B, 240, False) 00:09:52.4 --- Valves A and B switched to MixTee, starting gradient 00:09:52.4 --- LoadGradient() --- 00:09:52.4 --- Loading gradient method index: 0 00:09:52.4 pump: LoadMethod(0, ZrData.ZrPumpMethod) 00:09:52.9 pump: GetMethodState(0) --> loaded 00:09:52.9 --- Set gradient start 00:09:52.9 --- StartGradient() --- 00:09:52.9 --- Starting gradient method index: 0 00:09:52.9 pump: SetMethodSignal(0, StartTrunk) 00:09:53.1 pump: GetMethodState(0) --> runningTrunk 00:09:53.1 --- Start GradientSplineFit 00:09:53.1 pump: GetMethodState(0) --> runningTrunk 00:09:54.1 pump: GetCurrentPressure(A) --> 90.96442 00:09:54.1 pump: GetCurrentPressure(B) --> 13.98148 00:09:54.1 --- GradientSplineFit is running 00:10:52.1 --- GradientSplineFit has finished 00:10:52.1 --- End GradientSplineFit 00:10:52.1 --- Finished spline flow reduction A --- 00:10:52.1 --- Finished conditioning paths --- 00:10:57.4 autosampler-none: MoveValveDrive(Valve Drive 2, 240 deg) 00:10:57.4 pump: GetSetExternalTemperature() --> 50 00:10:57.4 --- Oven temperatur: 50 00:10:57.4 --- Separator: Name: IonOpticks 25 cm, Func: Separator, ColDia: 0.075 mm, Len: 250 mm, PartDia: 1.6 µm, Porosity: 42 % 00:10:57.4 --- Spline fitting time: 97.6577595902957 s 00:10:57.4 --- Spline fitting time adapted: 53.7117677746626 s 00:10:57.4 --- Press: 541.893115083548; colPress: 511.893115083548, visco: 0.00566684100509561, flow > 0.3 00:10:57.4 --- Flow: 0.3; Mix: 0, calcI: 220.674819441516 00:10:57.4 --- Ki calc: 220.674819441516 00:10:57.4 pump: GetSettings() --> PumpSettings 00:10:57.4 ------------------------------------------------------------------ 00:10:57.5 PID settings changed: 00:10:57.5 ------------------------------------------------------------------ 00:10:57.5 P: 200 : True; Pmin: -32000 : True; Pmax: 32000 : True 00:10:57.5 I: 600 : True; Imin: -32000 : True; Imax: 32000 : True; IWindow: 100 : True 00:10:57.5 D: 1000 : True; Dmin: -32000 : True; Dmax: 32000 : True 00:10:57.5 Result min: -32000 : True; Result max: 32000 : True 00:10:57.5 ------------------------------------------------------------------ 00:10:57.5 pump: SetSettings(PumpSettings) 00:10:57.5 pump: GetSettings() --> PumpSettings 00:10:57.5 ------------------------------------------------------------------ 00:10:57.5 PID settings changed: 00:10:57.5 ------------------------------------------------------------------ 00:10:57.5 P: 200 : False; Pmin: -32000 : False; Pmax: 32000 : False 00:10:57.5 I: 220 : True; Imin: -32000 : False; Imax: 32000 : False; IWindow: 100 : False 00:10:57.5 D: 1000 : False; Dmin: -32000 : False; Dmax: 32000 : False 00:10:57.5 Result min: -32000 : False; Result max: 32000 : False 00:10:57.5 ------------------------------------------------------------------ 00:10:57.5 pump: SetSettings(PumpSettings) 00:10:57.5 --- y = -5.33333333333333E-05x + 0.302864627614649 00:10:57.5 --- p0.Time -1, p0.Flow 0.01 00:10:57.5 --- p1.Time 0, p1.Flow 0.01 00:10:57.5 --- p2.Time 52.7117677746626, p2.Flow 0.300053333333333 00:10:57.5 --- p3.Time 53.7117677746626, p3.Flow 0.3, p3.Mix 0 00:10:57.5 --- p4.Time 1853.71176777466, p4.Flow 0.204, p4.Mix 32 00:10:57.5 --- Strategy function end 00:10:57.5 --- y = 5.33333333333333E-05x + -0.00286462761464867 00:10:57.5 --- p0.Time -1, p0.Flow 0 00:10:57.5 --- p1.Time 0, p1.Flow 0 00:10:57.5 --- p2.Time 52.7117677746626, p2.Flow -5.33333333333336E-05 00:10:57.5 --- p3.Time 53.7117677746626, p3.Flow 0, p3.Mix 0 00:10:57.5 --- p4.Time 1853.71176777466, p4.Flow 0.096, p4.Mix 32 00:10:57.5 --- Strategy function end 00:10:57.5 xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx 00:10:57.5 XXXXX Time 0, Flow 0.01, Mix 0 00:10:57.5 XXXXX Time 1, Flow 0.01059, Mix 0 00:10:57.5 XXXXX Time 2, Flow 0.01175, Mix 0 00:10:57.6 XXXXX Time 3, Flow 0.01347, Mix 0 00:10:57.6 XXXXX Time 4, Flow 0.01571, Mix 0 00:10:57.6 XXXXX Time 5, Flow 0.01845, Mix 0 00:10:57.6 XXXXX Time 6, Flow 0.02168, Mix 0 00:10:57.6 XXXXX Time 7, Flow 0.02537, Mix 0 00:10:57.7 XXXXX Time 8, Flow 0.02949, Mix 0 00:10:57.7 XXXXX Time 9, Flow 0.03402, Mix 0 00:10:57.7 XXXXX Time 10, Flow 0.03894, Mix 0 00:10:57.7 XXXXX Time 11, Flow 0.04424, Mix 0 00:10:57.7 XXXXX Time 12, Flow 0.04988, Mix 0 00:10:57.8 XXXXX Time 13, Flow 0.05584, Mix 0 00:10:57.8 XXXXX Time 14, Flow 0.0621, Mix 0 00:10:57.8 XXXXX Time 15, Flow 0.06864, Mix 0 00:10:57.8 XXXXX Time 16, Flow 0.07544, Mix 0 00:10:57.8 XXXXX Time 17, Flow 0.08247, Mix 0 00:10:57.9 XXXXX Time 18, Flow 0.08971, Mix 0 00:10:57.9 XXXXX Time 19, Flow 0.09713, Mix 0 00:10:57.9 XXXXX Time 20, Flow 0.10472, Mix 0 00:10:57.9 XXXXX Time 21, Flow 0.11246, Mix 0 00:10:57.9 XXXXX Time 22, Flow 0.12031, Mix 0 00:10:58.0 XXXXX Time 23, Flow 0.12826, Mix 0 00:10:58.0 XXXXX Time 24, Flow 0.13629, Mix 0 00:10:58.0 XXXXX Time 25, Flow 0.14437, Mix 0 00:10:58.0 XXXXX Time 26, Flow 0.15248, Mix 0 00:10:58.0 XXXXX Time 27, Flow 0.16059, Mix 0 00:10:58.1 XXXXX Time 28, Flow 0.16869, Mix 0 00:10:58.1 XXXXX Time 29, Flow 0.17675, Mix 0 00:10:58.1 XXXXX Time 30, Flow 0.18476, Mix 0 00:10:58.1 XXXXX Time 31, Flow 0.19267, Mix 0 00:10:58.1 XXXXX Time 32, Flow 0.20049, Mix 0 00:10:58.2 XXXXX Time 33, Flow 0.20817, Mix 0 00:10:58.2 XXXXX Time 34, Flow 0.2157, Mix 0 00:10:58.2 XXXXX Time 35, Flow 0.22306, Mix 0 00:10:58.2 XXXXX Time 36, Flow 0.23022, Mix 0 00:10:58.2 XXXXX Time 37, Flow 0.23717, Mix 0 00:10:58.3 XXXXX Time 38, Flow 0.24387, Mix 0 00:10:58.3 XXXXX Time 39, Flow 0.25031, Mix 0 00:10:58.3 XXXXX Time 40, Flow 0.25646, Mix 0 00:10:58.3 XXXXX Time 41, Flow 0.2623, Mix 0 00:10:58.3 XXXXX Time 42, Flow 0.26781, Mix 0 00:10:58.3 XXXXX Time 43, Flow 0.27297, Mix 0 00:10:58.4 XXXXX Time 44, Flow 0.27775, Mix 0 00:10:58.4 XXXXX Time 45, Flow 0.28213, Mix 0 00:10:58.4 XXXXX Time 46, Flow 0.28609, Mix 0 00:10:58.4 XXXXX Time 47, Flow 0.28961, Mix 0 00:10:58.4 XXXXX Time 48, Flow 0.29265, Mix 0 00:10:58.5 XXXXX Time 49, Flow 0.29521, Mix 0 00:10:58.5 XXXXX Time 50, Flow 0.29726, Mix 0 00:10:58.5 XXXXX Time 51, Flow 0.29877, Mix 0 00:10:58.5 XXXXX Time 52, Flow 0.29972, Mix 0 00:10:58.5 XXXXX Time 53, Flow 0.3001, Mix 0 00:10:58.5 --- Gradient is created, switching valves A and B 00:10:58.5 pump: GetSetManualValvePosition(A) --> 240 00:10:58.5 pump: GetCurrentPressure(A) --> 0 00:10:58.5 pump: SetManualValvePosition(A, 240, False) 00:10:58.7 pump: GetSetManualValvePosition(B) --> 240 00:10:58.7 pump: GetCurrentPressure(B) --> 0 00:10:58.7 pump: SetManualValvePosition(B, 240, False) 00:10:58.9 --- Valves A and B switched to MixTee, starting gradient 00:10:58.9 --- LoadGradient() --- 00:10:58.9 --- Loading gradient method index: 0 00:10:58.9 pump: LoadMethod(0, ZrData.ZrPumpMethod) 00:10:59.4 pump: GetMethodState(0) --> loaded 00:10:59.4 --- Set gradient start 00:10:59.4 --- StartGradient() --- 00:10:59.4 --- Starting gradient method index: 0 00:10:59.4 pump: SetMethodSignal(0, StartTrunk) 00:10:59.6 pump: GetMethodState(0) --> runningTrunk 00:10:59.6 --- Start GradientSplineFit 00:10:59.6 pump: GetMethodState(0) --> runningTrunk 00:11:00.6 pump: GetCurrentPressure(A) --> 0 00:11:00.6 pump: GetCurrentPressure(B) --> 0 00:11:00.6 --- GradientSplineFit is running 00:11:52.7 --- GradientSplineFit has finished 00:11:52.7 --- End GradientSplineFit 00:11:52.7 status: RemoveStatus(preparing gradient start) 00:11:52.8 Setting signal "lc-ready" 00:11:52.8 Waiting for signal "ms-start"... 00:11:52.9 --- LoadGradient() --- 00:11:52.9 --- Loading gradient method index: 0 00:11:52.9 pump: LoadMethod(0, ZrData.ZrPumpMethod) 00:11:53.4 pump: GetMethodState(0) --> loaded 00:11:53.4 --- Set gradient start 00:11:53.4 --- StartGradient() --- 00:11:53.4 --- Starting gradient method index: 0 00:11:53.4 pump: SetMethodSignal(0, StartTrunk) 00:11:53.6 pump: GetMethodState(0) --> runningTrunk 00:11:53.6 autosampler-none: SetSignal(Output Signal 1) 00:11:53.6 status: SetStatus(wash, ) 00:11:57.3 status: SetStatus(elution, ) 00:11:58.9 autosampler-left: MoveToObject(LCInjector1) 00:12:01.6 autosampler-left: PenetrateWithConstForce(LCInjector1) 00:12:01.7 autosampler-left: MoveValveDrive(Valve Drive 1, 0 deg) 00:12:31.1 autosampler-left: CleanInjector(LCInjector1, LCP 1, 100 uL, 0 mm, 5 uL/s, 5 s, 20 mm, 40 uL/s, 1000 ms, 2, False) 00:14:01.1 autosampler-left: CleanInjector(LCInjector1, LCP 1, 300 uL, 0 mm, 5 uL/s, 5 s, 20 mm, 40 uL/s, 1000 ms, 1, True) 00:14:01.3 autosampler-left: MoveValveDrive(Valve Drive 1, 60 deg) 00:14:04.3 autosampler-left: MoveToHome() 01:11:54.3 Setting signal "lc-done" 01:11:54.3 status: RemoveStatus(elution) 01:11:54.3 status: RemoveStatus(wash) 01:11:54.3 pump: GetCurrentPressure(A) --> 243.9324 01:11:54.3 pump: GetCurrentPressure(B) --> 252.1005 01:11:54.3 pump: GetSettings() --> PumpSettings 01:11:54.3 pump: GetSetManualValvePosition(A) --> 240 01:11:54.3 pump: GetSetManualValvePosition(B) --> 240 01:11:54.3 ------------------------------------------------------- 01:11:54.3 --- Reduce pressure --- 01:11:54.3 ------------------------------------------------------- 01:11:54.3 --- Pressure channel A: 243.93244934082 01:11:54.3 --- Pressure channel B: 252.100463867188 01:11:54.3 pump: GetSettings() --> PumpSettings 01:11:54.3 ------------------------------------------------------------------ 01:11:54.3 PID settings changed: 01:11:54.3 ------------------------------------------------------------------ 01:11:54.3 P: 250 : True; Pmin: -5500 : True; Pmax: 32000 : True 01:11:54.3 I: 50 : True; Imin: -20000 : True; Imax: 20000 : True; IWindow: 50 : True 01:11:54.3 D: 1500 : True; Dmin: -10000 : True; Dmax: 10000 : True 01:11:54.3 Result min: -32000 : True; Result max: 32000 : True 01:11:54.3 ------------------------------------------------------------------ 01:11:54.3 pump: SetSettings(PumpSettings) 01:11:54.5 --- Set pressure channel A: 10 01:11:54.5 --- Set pressure channel B: 5 01:11:54.5 --- Reduce pressure flow controlled: True 01:12:24.8 -------------------------------------------------------- 01:12:24.8 --- Log PID parameter called from: reducePressure 01:12:24.8 -------------------------------------------------------- 01:12:24.8 --- P: 200; Pmin: -32000; Pmax: 32000 01:12:24.8 --- I: 220; Imin: -32000; Imax: 32000; IWindow: 100 01:12:24.8 --- D: 1000; Dmin: -32000; Dmax: 32000 01:12:24.8 --- Result min: -32000; Result max: 32000 01:12:24.8 -------------------------------------------------------- 01:12:24.8 -------------------------------------------------------- 01:12:24.8 --- Log instrument settings called from: reducePressure 01:12:24.8 -------------------------------------------------------- 01:12:24.8 --- HomeSpeed: 2000; PurgeSpeed: 2000 01:12:24.8 --- PressureTrackingExcessFactor: 96 01:12:24.8 --- FlowResistanceA: 300; FlowResistanceB: 100; FlowResistanceOut: 150 01:12:24.8 --- FC_FlowFilterDepth: 5; FC_PressureFilterDepth: 0 01:12:24.8 --- PC_OutputFilterDepth: 0 01:12:24.8 --- CompressibilityFactorB: 2.29999995231628; PressurePIDNegativeResultDivisor: 1 01:12:24.8 --- ExternalZeroPressureOffsetA: 130.944732666016; ExternalZeroPressureOffsetB: 128.061676025391 01:12:24.8 --- ExternalPressureScalingFactorA: 0.0197199992835522; ExternalPressureScalingFactorB: 0.0197199992835522 01:12:24.8 -------------------------------------------------------- 01:12:24.8 --- Final pressure channel A: 5.69514226913452 01:12:24.8 --- Final pressure channel B: 4.43699932098389 01:12:24.8 ------------------------------------------------------- 01:12:25.1 autosampler-left: MoveValveDrive(Valve Drive 2, 0 deg) 01:12:25.3 pump: Manualmode_Pump_constantSpeed(A, 0) 01:12:25.3 pump: GetSetPistonSpeed(A) --> 0 01:12:25.3 pump: Manualmode_Pump_constantSpeed(B, 0) 01:12:25.3 pump: GetSetPistonSpeed(B) --> 0 01:12:25.3 pump: IsIdle() --> True 01:12:25.4 autosampler-left: MoveValveDrive(Valve Drive 1, 60 deg) 01:12:27.7 autosampler-left: MoveToHome() 01:12:27.8 pump: IsIdle() --> True 01:12:27.8 COMPLETED