Path: 2022-08-28_15-58-00_Two-column-separation
Procedure: Two column separation
Argument list:
uses_trap = True
uses_separator = True
is_isocratic = False
analysis_time = 1800
oven_temperature = 50
sample_volume = 5
sample_position = Slot2:28
presample_solvent_volume = 2
presample_air_volume = 0.5
postsample_air_volume = 1
postsample_solvent_volume = 2
sample_aspirate_speed = 5
sample_postaspirate_delay = 500
sample_inject_speed = 1
calibrant_volume = 0.6
calibrantTime = 10
is_calibrant_inject = False
is_extended_wash = False
is_bottom_sense = True
injection_method = uLPickup
trap = Name: Thermo Trap Cartridge 5mm, Func: Separator, ColDia: 0.3 mm, Len: 5 mm, PartDia: 5 µm, Porosity: 42 %
trap_volume = 0.148440252882118
trap_unityflow = 2.78838695282287
trap_equilibration_volumemultiplier = 10
trap_equilibration_pressure = 217.5
trap_equil_time = 0.648440252882118
separator = Name: IonOpticks 25 cm, Func: Separator, ColDia: 0.075 mm, Len: 250 mm, PartDia: 1.6 µm, Porosity: 42 %
separator_volume = 0.463875790256618
separator_unityflow = 0.00058605984562038
separator_equilibration_volumemultiplier = 4
separator_equilibration_pressure = 800
column_load_volumemultiplier = 4
column_load_pressure = 217.5
separator_equil_time = 4.05402026133927
column_load_time = 0.6
gradient = (0 sec,0.3 µL/min,0.00 %), (1800 sec,0.3 µL/min,32.00 %), (2700 sec,0.3 µL/min,40.00 %), (3000 sec,0.3 µL/min,60.00 %), (3300 sec,0.3 µL/min,95.00 %), (3600 sec,0.3 µL/min,95.00 %)
00:00:00.0	STARTED
00:00:00.4	Lua version:		1.1.0
00:00:00.4	Lua date:			2020/01/30
00:00:00.4	--- Experiment:    Description
00:00:00.4	-------------------------------------------------------
00:00:00.4	--- Trap Column:              Thermo Trap Cartridge 5mm
00:00:00.4	---    Manufacturer:          Thermo Scientific
00:00:00.4	---    Part Number:           
00:00:00.4	---    Serial Number:         
00:00:00.4	---    Type/Material:         µ-Precolum TM Cartridge  / PepMap TM C18
00:00:00.4	---    Length:                5 mm
00:00:00.4	---    Inner Diameter:        0.3 mm
00:00:00.4	---    Particle Diameter:     5 um
00:00:00.4	---    Is Particle Pore Size: True
00:00:00.4	---    Particle Pore Size:    100
00:00:00.4	---    Is Column Porosity:    False
00:00:00.4	---    Column Porosity:       0.42 (0.0-1.0)
00:00:00.4	---    Is Max Temperature:    False
00:00:00.4	---    max Temperature:       65 C
00:00:00.4	---    Is Max Pressure:       False
00:00:00.4	---    max Pressure:          1000 bar
00:00:00.4	---    Is Max Flow:           False
00:00:00.4	---    max Flow:              9 uL/min
00:00:00.4	---    Advanced Settings:     False
00:00:00.4	---    Column Comments:       Manufacturer no: 160454
00:00:00.4	-------------------------------------------------------
00:00:00.4	--- Separation Column:        IonOpticks 25 cm
00:00:00.4	---    Manufacturer:          IonOpticks
00:00:00.4	---    Part Number:           
00:00:00.4	---    Serial Number:         
00:00:00.4	---    Type/Material:         C18
00:00:00.4	---    Length:                250 mm
00:00:00.4	---    Inner Diameter:        0.075 mm
00:00:00.4	---    Particle Diameter:     1.6 um
00:00:00.4	---    Is Particle Pore Size: True
00:00:00.4	---    Particle Pore Size:    120
00:00:00.4	---    Is Column Porosity:    False
00:00:00.4	---    Column Porosity:       0.42 (0.0-1.0)
00:00:00.4	---    Is Max Temperature:    False
00:00:00.4	---    max Temperature:       65 C
00:00:00.4	---    Is Max Pressure:       True
00:00:00.4	---    max Pressure:          1000 bar
00:00:00.4	---    Is Max Flow:           False
00:00:00.4	---    max Flow:              9 uL/min
00:00:00.4	---    Advanced Settings:     False
00:00:00.4	---    Column Comments:           
00:00:00.4	-------------------------------------------------------
00:00:00.4	'Valve Drive 1' is 'ILcInjectorValve'
00:00:00.4	'Valve Drive 2' is 'ISelectorValve'
00:00:00.4	--- Trap equil time:		0.64
00:00:00.4	--- Seperator equil time:	4.05
00:00:00.4	--- Column loading time:	0.6
00:00:00.4	pump: GetSettings() --> PumpSettings
00:00:00.4	--------------------------------------------------------
00:00:00.4	--- Log instrument settings called from: Two Column Separation
00:00:00.4	--------------------------------------------------------
00:00:00.4	--- HomeSpeed: 2000; PurgeSpeed: 2000
00:00:00.4	--- PressureTrackingExcessFactor: 96
00:00:00.4	--- FlowResistanceA: 300; FlowResistanceB: 100; FlowResistanceOut: 150
00:00:00.4	--- FC_FlowFilterDepth: 5; FC_PressureFilterDepth: 0
00:00:00.4	--- PC_OutputFilterDepth: 0
00:00:00.4	--- CompressibilityFactorB: 2.29999995231628; PressurePIDNegativeResultDivisor: 1
00:00:00.4	--- ExternalZeroPressureOffsetA: 130.700210571289; ExternalZeroPressureOffsetB: 127.99462890625
00:00:00.4	--- ExternalPressureScalingFactorA: 0.0197199992835522; ExternalPressureScalingFactorB: 0.0197199992835522
00:00:00.4	--------------------------------------------------------
00:00:00.4	--- Oven temperature: 50
00:00:00.4	--- Separator: Name: IonOpticks 25 cm, Func: Separator, ColDia: 0.075 mm, Len: 250 mm, PartDia: 1.6 µm, Porosity: 42 % ---
00:00:00.4	--- Trap: Name: Thermo Trap Cartridge 5mm, Func: Separator, ColDia: 0.3 mm, Len: 5 mm, PartDia: 5 µm, Porosity: 42 % ---
00:00:00.4	--- MaxPressureLimitAB: 1000 bar ---
00:00:00.4	pump: GetCurrentPressure(A) --> 6.689024
00:00:00.4	pump: GetCurrentPressure(B) --> 6.219686
00:00:00.4	pump: GetSettings() --> PumpSettings
00:00:00.4	pump: GetSetManualValvePosition(A) --> 240
00:00:00.4	pump: GetSetManualValvePosition(B) --> 240
00:00:00.4	-------------------------------------------------------
00:00:00.4	--- Reduce pressure ---
00:00:00.4	-------------------------------------------------------
00:00:00.4	--- Pressure channel A: 6.68902444839478
00:00:00.4	--- Pressure channel B: 6.21968603134155
00:00:00.4	pump: Manualmode_Pump_constantSpeed(A, 0)
00:00:00.4	pump: GetSetPistonSpeed(A) --> 0
00:00:00.4	pump: Manualmode_Pump_constantSpeed(B, 0)
00:00:00.4	pump: GetSetPistonSpeed(B) --> 0
00:00:00.4	pump: IsIdle() --> True
00:00:00.4	-------------------------------------------------------
00:00:00.5	pump: SetMaxPressureLimit(A, 1020)
00:00:00.5	pump: GetMaxPressureLimit(A) --> 1020
00:00:00.6	pump: SetMaxPressureLimit(B, 1020)
00:00:00.6	pump: GetMaxPressureLimit(B) --> 1020
00:00:00.6	pump: GetSettings() --> PumpSettings
00:00:00.6	------------------------------------------------------------------
00:00:00.6	PID settings changed:
00:00:00.6	------------------------------------------------------------------
00:00:00.6	P: 175 : True; Pmin: -5000 : True; Pmax: 5000 : True
00:00:00.6	I: 5 : True; Imin: -200 : True; Imax: 1000 : True; IWindow: 10 : True
00:00:00.6	D: 0 : True; Dmin: -1000 : True; Dmax: 1000 : True
00:00:00.6	Result min: -6000 : True; Result max: 8000 : True
00:00:00.6	------------------------------------------------------------------
00:00:00.6	pump: SetSettings(PumpSettings)
00:00:00.6	pump: GetPumpSideState(A) --> manualMode
00:00:00.6	pump: GetPumpSideState(B) --> manualMode
00:00:00.6	pump: GetPistonPosition(A) --> 818.450021947826
00:00:00.6	pump: GetPistonPosition(B) --> 115.612494607697
00:00:00.6	-------------------------------------------------------
00:00:00.6	--- Optional Refill of Pump Heads:
00:00:00.6	--- initiated by channel A:     False, B: False
00:00:00.6	pump: GetPistonPosition(A) --> 818.450021947826
00:00:00.6	pump: GetPistonPosition(B) --> 115.612494607697
00:00:00.6	--- Piston position channel A:  818.450021947826, B: 115.612494607697
00:00:00.6	-------------------------------------------------------
00:00:00.6	pump: SetDigitalOutput(PO2, False)
00:00:00.6	pump: GetCurrentPressure(A) --> 6.669304
00:00:00.6	pump: GetCurrentPressure(B) --> 6.199966
00:00:00.6	pump: GetSettings() --> PumpSettings
00:00:00.6	pump: GetSetManualValvePosition(A) --> 240
00:00:00.6	pump: GetSetManualValvePosition(B) --> 240
00:00:00.6	-------------------------------------------------------
00:00:00.6	--- Reduce pressure ---
00:00:00.6	-------------------------------------------------------
00:00:00.6	--- Pressure channel A: 6.66930437088013
00:00:00.6	--- Pressure channel B: 6.19996643066406
00:00:00.6	pump: GetSettings() --> PumpSettings
00:00:00.6	------------------------------------------------------------------
00:00:00.6	PID settings changed:
00:00:00.6	------------------------------------------------------------------
00:00:00.6	P: 250 : True; Pmin: -5500 : True; Pmax: 32000 : True
00:00:00.6	I: 50 : True; Imin: -20000 : True; Imax: 20000 : True; IWindow: 50 : True
00:00:00.6	D: 1500 : True; Dmin: -10000 : True; Dmax: 10000 : True
00:00:00.6	Result min: -32000 : True; Result max: 32000 : True
00:00:00.6	------------------------------------------------------------------
00:00:00.6	pump: SetSettings(PumpSettings)
00:00:00.9	--- Set pressure channel A:          10
00:00:00.9	--- Set pressure channel B:          5
00:00:00.9	--- Reduce pressure flow controlled: True
00:00:01.9	--------------------------------------------------------
00:00:01.9	--- Log PID parameter called from: reducePressure
00:00:01.9	--------------------------------------------------------
00:00:01.9	--- P: 175; Pmin: -5000; Pmax: 5000
00:00:01.9	--- I: 5; Imin: -200; Imax: 1000; IWindow: 10
00:00:01.9	--- D: 0; Dmin: -1000; Dmax: 1000
00:00:01.9	--- Result min: -6000; Result max: 8000
00:00:01.9	--------------------------------------------------------
00:00:01.9	--------------------------------------------------------
00:00:01.9	--- Log instrument settings called from: reducePressure
00:00:01.9	--------------------------------------------------------
00:00:01.9	--- HomeSpeed: 2000; PurgeSpeed: 2000
00:00:01.9	--- PressureTrackingExcessFactor: 96
00:00:01.9	--- FlowResistanceA: 300; FlowResistanceB: 100; FlowResistanceOut: 150
00:00:01.9	--- FC_FlowFilterDepth: 5; FC_PressureFilterDepth: 0
00:00:01.9	--- PC_OutputFilterDepth: 0
00:00:01.9	--- CompressibilityFactorB: 2.29999995231628; PressurePIDNegativeResultDivisor: 1
00:00:01.9	--- ExternalZeroPressureOffsetA: 130.700210571289; ExternalZeroPressureOffsetB: 127.99462890625
00:00:01.9	--- ExternalPressureScalingFactorA: 0.0197199992835522; ExternalPressureScalingFactorB: 0.0197199992835522
00:00:01.9	--------------------------------------------------------
00:00:01.9	--- Final pressure channel A: 6.82706451416016
00:00:01.9	--- Final pressure channel B: 0
00:00:01.9	-------------------------------------------------------
00:00:01.9	pump: GetSetManualValvePosition(A) --> 240
00:00:01.9	pump: GetCurrentPressure(A) --> 6.827065
00:00:01.9	pump: SetManualValvePosition(A, 0, True)
00:00:02.5	pump: GetSetManualValvePosition(B) --> 240
00:00:02.5	pump: GetCurrentPressure(B) --> 0
00:00:02.5	pump: SetManualValvePosition(B, 180, False)
00:00:02.9	pump: GetSettings() --> PumpSettings
00:00:02.9	------------------------------------------------------------------
00:00:02.9	PID settings changed:
00:00:02.9	------------------------------------------------------------------
00:00:02.9	P: 250 : True; Pmin: -10000 : True; Pmax: 10000 : True
00:00:02.9	I: 20 : True; Imin: -10000 : True; Imax: 20000 : True; IWindow: 10 : True
00:00:02.9	D: 1500 : True; Dmin: -10000 : True; Dmax: 10000 : True
00:00:02.9	Result min: -10000 : True; Result max: 32000 : True
00:00:02.9	------------------------------------------------------------------
00:00:02.9	pump: SetSettings(PumpSettings)
00:00:02.9	status: SetStatus(equilibrate separation-column, this finishes at about: 4.84 min., )
00:00:03.5	autosampler-none: MoveValveDrive(Valve Drive 2, 180 deg)
00:00:03.5	--- MaxPressureLimitA: 800 bar ---
00:00:03.5	pump: GetCurrentPressure(A) --> 6.807344
00:00:03.5	pump: GetCurrentPressure() --> 6.807344
00:00:03.5	pump: GetSettings() --> PumpSettings
00:00:03.5	pump: GetSetManualValvePosition(A) --> 0
00:00:03.5	-------------------------------------------------------
00:00:03.5	--- Reduce pressure ---
00:00:03.5	-------------------------------------------------------
00:00:03.5	--- Pressure channel A: 6.80734443664551
00:00:03.5	--- Pressure channel B: 6.80734443664551
00:00:03.5	pump: Manualmode_Pump_constantSpeed(A, 0)
00:00:03.5	pump: GetSetPistonSpeed(A) --> 0
00:00:03.5	pump: Manualmode_Pump_constantSpeed(, 0)
00:00:03.5	pump: GetSetPistonSpeed() --> 0
00:00:03.5	pump: IsIdle() --> True
00:00:03.5	-------------------------------------------------------
00:00:03.6	pump: SetMaxPressureLimit(A, 820)
00:00:03.6	pump: GetMaxPressureLimit(A) --> 820
00:00:03.6	-------------------------------------------------------
00:00:03.6	--- Separation Column Equilibration:
00:00:03.6	-------------------------------------------------------
00:00:03.6	--- ovenTemp [C]:                                 50
00:00:03.6	--- Separator Unity-Flow @ ovenTemp [ul/min/bar]: 0.00058605984562038
00:00:03.6	--- Separator Volume [ul]:                        0.463875790256618
00:00:03.6	--- Separator Equilibration Volume Multiplier:    4
00:00:03.6	--- Separator Equilibration Pressure [bar]:       800
00:00:03.6	--- Separator Equilibration Flow [ul/min]:        0.457694594849779
00:00:03.6	--- Separator Equilibration Volume [ul]:          1.85550316102647
00:00:03.6	--- Separator Equilibration Time [min]:           4.05402026133927
00:00:03.6	--- max. Volume (10*V+2ul/min*t) [ul]:            26.6630721329433
00:00:03.6	--- Separator Equilibration Time Out [min]:       40.5402026133927
00:00:03.6	-------------------------------------------------------
00:00:03.6	pump: Manualmode_Pump_constantPressure(A, 800)
00:00:03.6	pump: GetPistonPosition(A) --> 818.450021947826
00:00:03.6	--- Injection delay: 237.164855895902s
00:00:22.6	pump: GetPistonPosition(A) --> 881.621167026209
00:00:22.6	pump: GetPistonPosition(A) --> 881.621167026209
00:00:22.6	pump: GetPistonPosition(A) --> 881.621167026209
00:04:01.0	status: SetStatus(aspirate & inject, )
00:04:01.5	autosampler-left: LeaveObject()
00:04:05.6	autosampler-left: MoveToObject(LCMS Wash 1, 3)
00:04:06.2	autosampler-left: PenetrateObject(LCMS Wash 1, 3)
00:04:06.3	autosampler-left: EmptySyringe()
00:04:07.1	autosampler-left: LeaveObject()
00:04:08.0	Cleaning syringe and needle exterior
00:04:23.3	autosampler-left: CleanSyringe(LCMS Wash 1, 1, 30 mm, 40 uL/s, 500 ms, 30 mm, 40 uL/s, 80 %, 2)
00:04:23.4	autosampler-left: ChangeTool(LCP 1) --> False
00:04:26.4	pump: GetPistonPosition(A) --> 889.701133891732
00:04:26.4	pump: GetCurrentPressure(A) --> 799.9852
00:04:26.4	--- act. pressure [bar]:                 799.985168457031
00:04:26.4	--- used volume [uL]:                    8.07996686552281
00:04:26.4	--- average flow (incl. leaks) [uL/min]: 1.98873542462325
00:04:26.4	--- act. equilibration time [min]:       4.06286666666759
00:04:26.4	-------------------------------------------------------
00:04:26.4	status: RemoveStatus(equilibrate separation-column, this finishes at about: 4.84 min.)
00:04:26.4	status: SetStatus(equilibrate trap-column, this finishes at about: 5.58 min., )
00:04:26.8	autosampler-left: MoveToObject(Item Position 3, 1, True, True, True)
00:04:26.8	--- MaxPressureLimitA: 217.5 bar ---
00:04:26.8	pump: GetCurrentPressure(A) --> 799.926
00:04:26.8	pump: GetCurrentPressure() --> 799.926
00:04:26.8	pump: GetSettings() --> PumpSettings
00:04:26.8	pump: GetSetManualValvePosition(A) --> 0
00:04:26.8	-------------------------------------------------------
00:04:26.8	--- Reduce pressure ---
00:04:26.8	-------------------------------------------------------
00:04:26.8	--- Pressure channel A: 799.926025390625
00:04:26.8	--- Pressure channel B: 799.926025390625
00:04:26.8	pump: GetSettings() --> PumpSettings
00:04:26.8	------------------------------------------------------------------
00:04:26.8	PID settings changed:
00:04:26.8	------------------------------------------------------------------
00:04:26.8	P: 250 : True; Pmin: -32000 : True; Pmax: 32000 : True
00:04:26.8	I: 20 : True; Imin: -20000 : True; Imax: 20000 : True; IWindow: 100 : True
00:04:26.8	D: 1500 : True; Dmin: -10000 : True; Dmax: 10000 : True
00:04:26.8	Result min: -32000 : True; Result max: 32000 : True
00:04:26.8	------------------------------------------------------------------
00:04:26.8	pump: SetSettings(PumpSettings)
00:04:26.9	--- Set pressure channel A:          217.5
00:04:26.9	--- Set pressure channel B:          217.5
00:04:26.9	--- Reduce pressure flow controlled: False
00:04:30.0	autosampler-left: PenetrateWithBottomSense(Item Position 3, 1, 1 mm)
00:04:31.0	autosampler-left: AspirateSyringe(2 uL, 5 uL/s, 500 ms)
00:04:32.2	autosampler-left: LeaveObject()
00:04:32.9	autosampler-left: AspirateSyringe(0.5 uL, 5 uL/s, 500 ms)
00:04:39.1	--------------------------------------------------------
00:04:39.1	--- Log PID parameter called from: reducePressure
00:04:39.1	--------------------------------------------------------
00:04:39.1	--- P: 250; Pmin: -10000; Pmax: 10000
00:04:39.1	--- I: 20; Imin: -10000; Imax: 20000; IWindow: 10
00:04:39.1	--- D: 1500; Dmin: -10000; Dmax: 10000
00:04:39.1	--- Result min: -10000; Result max: 32000
00:04:39.1	--------------------------------------------------------
00:04:39.1	--------------------------------------------------------
00:04:39.1	--- Log instrument settings called from: reducePressure
00:04:39.1	--------------------------------------------------------
00:04:39.1	--- HomeSpeed: 2000; PurgeSpeed: 2000
00:04:39.1	--- PressureTrackingExcessFactor: 96
00:04:39.1	--- FlowResistanceA: 300; FlowResistanceB: 100; FlowResistanceOut: 150
00:04:39.1	--- FC_FlowFilterDepth: 5; FC_PressureFilterDepth: 0
00:04:39.1	--- PC_OutputFilterDepth: 0
00:04:39.1	--- CompressibilityFactorB: 2.29999995231628; PressurePIDNegativeResultDivisor: 1
00:04:39.1	--- ExternalZeroPressureOffsetA: 130.700210571289; ExternalZeroPressureOffsetB: 127.99462890625
00:04:39.1	--- ExternalPressureScalingFactorA: 0.0197199992835522; ExternalPressureScalingFactorB: 0.0197199992835522
00:04:39.1	--------------------------------------------------------
00:04:39.1	--- Final pressure channel A: 216.529541015625
00:04:39.1	--- Final pressure channel B: 216.529541015625
00:04:39.1	-------------------------------------------------------
00:04:39.2	pump: SetMaxPressureLimit(A, 237.5)
00:04:39.2	pump: GetMaxPressureLimit(A) --> 237.5
00:04:39.4	autosampler-none: MoveValveDrive(Valve Drive 2, 60 deg)
00:04:39.4	-------------------------------------------------------
00:04:39.4	--- Trap Column Equilibration
00:04:39.4	-------------------------------------------------------
00:04:39.4	--- Trap Temperature: fixed to 20 C
00:04:39.4	--- Trap Unity-Flow  @ 20C [ul/bar]:      2.78838695282287
00:04:39.4	--- Trap Volume [ul]:                     0.148440252882118
00:04:39.4	--- Trap Equilibration Volume Multiplier: 10
00:04:39.4	--- Trap Equilibration Pressure [bar]:    217.5
00:04:39.4	--- Trap Equilibration Flow [ul/min]:     9.99557465783688
00:04:39.4	--- Trap Equilibration Volume (+5) [ul]:  6.48440252882118
00:04:39.4	--- Trap Equilibration Time [min]:        0.648727336925764
00:04:39.4	--- max. Volume (10*V+2ul/min*t) [ul]:    66.1414799620633
00:04:39.4	-------------------------------------------------------
00:04:39.4	pump: Manualmode_Pump_constantPressure(A, 217.5)
00:04:39.4	pump: GetPistonPosition(A) --> 845.511072747317
00:04:43.2	autosampler-left: MoveToObject(Rack 2, 28, True, True, True)
00:04:47.1	autosampler-left: PenetrateWithBottomSense(Rack 2, 28, 1 mm)
00:04:48.7	autosampler-left: AspirateSyringe(5 uL, 5 uL/s, 500 ms)
00:04:56.0	autosampler-left: LeaveObject()
00:04:56.9	autosampler-left: AspirateSyringe(1 uL, 5 uL/s, 500 ms)
00:04:58.4	pump: GetPistonPosition(A) --> 847.600022422444
00:04:58.4	pump: GetPistonPosition(A) --> 847.600022422444
00:04:58.4	pump: GetPistonPosition(A) --> 847.600022422444
00:05:00.5	autosampler-left: MoveToObject(LCMS Wash 1, 1, True, True, True)
00:05:01.5	autosampler-left: PenetrateObject(LCMS Wash 1, 1, 30 mm)
00:05:06.5	autosampler-left: Wait(5 s)
00:05:07.4	autosampler-left: LeaveObject()
00:05:10.4	autosampler-left: MoveToObject(LCInjector1)
00:05:11.3	autosampler-left: DispenseSyringe(0.75 uL, 1 uL/s)
00:05:13.9	autosampler-left: PenetrateWithConstForce(LCInjector1)
00:05:14.1	autosampler-left: MoveValveDrive(Valve Drive 1, 0 deg)
00:05:15.2	autosampler-left: Wait(1000 ms)
00:05:22.1	autosampler-left: DispenseSyringe(6.75 uL, 1 uL/s)
00:05:23.1	autosampler-left: Wait(1000 ms)
00:05:23.5	status: RemoveStatus(aspirate & inject)
00:05:37.5	pump: GetPistonPosition(A) --> 851.758025019225
00:05:37.5	pump: GetPistonPosition(A) --> 851.758025019225
00:05:59.6	pump: GetPistonPosition(A) --> 854.10751744509
00:05:59.6	pump: GetCurrentPressure(A) --> 217.4564
00:05:59.6	-------------------------------------------------------
00:05:59.6	--- Finished: Trap Column Equilibration (const_pressure_load_and_equilibration)
00:05:59.6	-------------------------------------------------------
00:05:59.6	--- act. pressure [bar]:           217.45637512207
00:05:59.6	--- used volume [uL]:              6.50749502264591
00:05:59.6	--- average flow [uL/min]:         6.38073116353243
00:05:59.6	--- act. equilibration time [min]: 1.01986666666635
00:05:59.6	-------------------------------------------------------
00:05:59.6	status: RemoveStatus(equilibrate trap-column, this finishes at about: 5.58 min.)
00:06:01.6	--- MaxPressureLimitA: 217.5 bar ---
00:06:01.6	pump: GetCurrentPressure(A) --> 217.4761
00:06:01.6	pump: GetCurrentPressure() --> 217.4761
00:06:01.6	pump: GetSettings() --> PumpSettings
00:06:01.6	pump: GetSetManualValvePosition(A) --> 0
00:06:01.6	-------------------------------------------------------
00:06:01.6	--- Reduce pressure ---
00:06:01.6	-------------------------------------------------------
00:06:01.6	--- Pressure channel A: 217.476089477539
00:06:01.6	--- Pressure channel B: 217.476089477539
00:06:01.6	pump: Manualmode_Pump_constantSpeed(A, 0)
00:06:01.6	pump: GetSetPistonSpeed(A) --> 0
00:06:01.6	pump: Manualmode_Pump_constantSpeed(, 0)
00:06:01.6	pump: GetSetPistonSpeed() --> 0
00:06:01.6	pump: IsIdle() --> False
00:06:01.6	-------------------------------------------------------
00:06:01.7	pump: SetMaxPressureLimit(A, 237.5)
00:06:01.7	pump: GetMaxPressureLimit(A) --> 237.5
00:06:01.7	status: SetStatus(load trap-column, this finishes at about: 8.30 min., )
00:06:01.8	autosampler-left: MoveValveDrive(Valve Drive 1, 60 deg)
00:06:03.2	-------------------------------------------------------
00:06:03.2	--- Sample Loading on Trap Column
00:06:03.2	-------------------------------------------------------
00:06:03.2	--- Trap Temperature: fixed to 20 C
00:06:03.2	--- Trap Unity-Flow [ul/bar]:          2.78838695282287
00:06:03.2	--- Trap Volume [ul]:                  0.148440252882118
00:06:03.2	--- Sample Volume [ul]:                5
00:06:03.2	--- Sample Volume Multiplier:          4
00:06:03.2	--- Trap Loading Pressure [bar]:       217.5
00:06:03.2	--- Trap Loading Flow [uL/min]:        9.99557465783688
00:06:03.2	--- Trap Loading Volume (+2) [uL]:     22
00:06:03.2	--- Trap Loading Time [min]:           2.200974006307
00:06:03.2	--- max. Volume (10*V+2ul/min*t) [ul]: 224.401948012614
00:06:03.2	-------------------------------------------------------
00:06:03.2	pump: Manualmode_Pump_constantPressure(A, 217.5)
00:06:03.2	pump: GetPistonPosition(A) --> 854.330570523495
00:06:03.3	autosampler-left: EmptySyringe(5 uL/s)
00:06:15.0	pump: GetPistonPosition(A) --> 856.413865331846
00:06:15.0	pump: GetPistonPosition(A) --> 856.413865331846
00:06:15.0	pump: GetPistonPosition(A) --> 856.413865331846
00:07:02.1	autosampler-left: CleanInjector(LCInjector1, LCP 1, 200 uL, 0 mm, 5 uL/s, 5 s, 20 mm, 40 uL/s, 1000 ms, 2, False)
00:08:03.6	autosampler-left: CleanInjector(LCInjector1, LCP 1, 200 uL, 0 mm, 5 uL/s, 5 s, 20 mm, 40 uL/s, 1000 ms, 1, True)
00:08:19.2	autosampler-left: CleanSyringe(LCMS Wash 1, 2, 30 mm, 40 uL/s, 500 ms, 30 mm, 40 uL/s, 80 %, 2)
00:08:27.1	pump: GetPistonPosition(A) --> 870.495111923766
00:08:27.1	pump: GetPistonPosition(A) --> 870.495111923766
00:08:39.7	autosampler-left: CleanSyringe(LCMS Wash 1, 1, 30 mm, 40 uL/s, 500 ms, 30 mm, 40 uL/s, 80 %, 3)
00:08:42.9	autosampler-left: MoveToHome()
00:09:42.4	pump: GetPistonPosition(A) --> 878.421559628283
00:09:42.4	pump: GetCurrentPressure(A) --> 217.5155
00:09:42.4	-------------------------------------------------------
00:09:42.4	--- Finished: Sample Loading on Trap Column (const_pressure_load_and_equilibration)
00:09:42.4	-------------------------------------------------------
00:09:42.4	--- act. pressure [bar]:     217.515533447266
00:09:42.4	--- used volume [uL]:        22.0076942964374
00:09:42.4	--- average flow [uL/min]:   6.36609435778463
00:09:42.4	--- act. loading time [min]: 3.45701666666719
00:09:42.4	-------------------------------------------------------
00:09:42.4	--- Separator: Name: IonOpticks 25 cm, Func: Separator, ColDia: 0.075 mm, Len: 250 mm, PartDia: 1.6 µm, Porosity: 42 % ---
00:09:42.4	--- Trap: Name: Thermo Trap Cartridge 5mm, Func: Separator, ColDia: 0.3 mm, Len: 5 mm, PartDia: 5 µm, Porosity: 42 % ---
00:09:42.4	--- MaxPressureLimitAB: 1000 bar ---
00:09:42.4	pump: GetCurrentPressure(A) --> 217.5155
00:09:42.4	pump: GetCurrentPressure(B) --> 0
00:09:42.4	pump: GetSettings() --> PumpSettings
00:09:42.4	pump: GetSetManualValvePosition(A) --> 0
00:09:42.4	-------------------------------------------------------
00:09:42.4	--- Reduce pressure ---
00:09:42.4	-------------------------------------------------------
00:09:42.4	--- Pressure channel A: 217.515533447266
00:09:42.4	--- Pressure channel B: 0
00:09:42.4	pump: Manualmode_Pump_constantSpeed(A, 0)
00:09:42.4	pump: GetSetPistonSpeed(A) --> 0
00:09:42.4	pump: Manualmode_Pump_constantSpeed(B, 0)
00:09:42.4	pump: GetSetPistonSpeed(B) --> 0
00:09:42.4	pump: IsIdle() --> False
00:09:42.4	-------------------------------------------------------
00:09:42.5	pump: SetMaxPressureLimit(A, 1020)
00:09:42.5	pump: GetMaxPressureLimit(A) --> 1020
00:09:42.6	pump: SetMaxPressureLimit(B, 1020)
00:09:42.6	pump: GetMaxPressureLimit(B) --> 1020
00:09:42.6	status: RemoveStatus(load trap-column, this finishes at about: 8.30 min.)
00:09:42.6	pump: GetSettings() --> PumpSettings
00:09:42.6	------------------------------------------------------------------
00:09:42.6	PID settings changed:
00:09:42.6	------------------------------------------------------------------
00:09:42.6	P: 100 : True; Pmin: -10000 : True; Pmax: 10000 : True
00:09:42.6	I: 100 : True; Imin: -1000 : True; Imax: 1000 : True; IWindow: 100 : True
00:09:42.6	D: 5000 : True; Dmin: -32000 : True; Dmax: 32000 : True
00:09:42.6	Result min: -5000 : True; Result max: 25000 : True
00:09:42.6	------------------------------------------------------------------
00:09:42.6	pump: SetSettings(PumpSettings)
00:09:42.6	pump: GetSettings() --> PumpSettings
00:09:42.6	------------------------------------------------------------------
00:09:42.6	PID settings changed:
00:09:42.6	------------------------------------------------------------------
00:09:42.6	P: 100 : False; Pmin: -10000 : False; Pmax: 10000 : False
00:09:42.6	I: 100 : False; Imin: -1000 : False; Imax: 1000 : False; IWindow: 2 : True
00:09:42.6	D: 5000 : False; Dmin: -32000 : False; Dmax: 32000 : False
00:09:42.6	Result min: -5000 : False; Result max: 25000 : False
00:09:42.6	------------------------------------------------------------------
00:09:42.6	pump: SetSettings(PumpSettings)
00:09:42.6	status: SetStatus(preparing gradient start, )
00:09:42.7	--- Starting conditioning paths ---
00:09:42.7	pump: GetSettings() --> PumpSettings
00:09:42.7	------------------------------------------------------------------
00:09:42.7	PID settings changed:
00:09:42.7	------------------------------------------------------------------
00:09:42.7	P: 100 : True; Pmin: -10000 : True; Pmax: 10000 : True
00:09:42.7	I: 100 : True; Imin: -1000 : True; Imax: 1000 : True; IWindow: 100 : True
00:09:42.7	D: 5000 : True; Dmin: -32000 : True; Dmax: 32000 : True
00:09:42.7	Result min: -5000 : True; Result max: 25000 : True
00:09:42.7	------------------------------------------------------------------
00:09:42.7	pump: SetSettings(PumpSettings)
00:09:42.8	pump: Manualmode_Pump_constantPressure(A, 0)
00:09:42.8	pump: GetTargetPressure(A) --> 0
00:09:42.8	pump: GetCurrentPressure(A) --> 217.4367
00:09:43.8	pump: GetCurrentPressure(A) --> 199.2745
00:09:44.8	pump: GetCurrentPressure(A) --> 172.4751
00:09:45.8	pump: GetCurrentPressure(A) --> 147.2729
00:09:46.8	pump: GetCurrentPressure(A) --> 120.572
00:09:47.8	pump: GetCurrentPressure(A) --> 94.6205
00:09:48.8	pump: GetCurrentPressure(A) --> 69.63526
00:09:49.8	pump: GetCurrentPressure(A) --> 44.86694
00:09:50.8	pump: GetCurrentPressure(A) --> 25.02862
00:09:51.8	pump: GetCurrentPressure(A) --> 14.41926
00:09:52.2	autosampler-none: MoveValveDrive(Valve Drive 2, 0 deg)
00:09:52.2	pump: SetManualValvePosition(A, 240, False)
00:09:52.8	pump: SetManualValvePosition(B, 240, False)
00:09:54.8	--- Set constant binary flow ---
00:09:54.8	pump: Manualmode_Pump_constantFlow_binary(1, 1)
00:09:54.8	pump: GetSetFlow(A) --> 1
00:09:54.8	pump: GetSetFlow(B) --> 1
00:09:54.8	

00:09:54.8	--- Starting spline flow reduction B/A ---
00:09:54.9	pump: GetSetExternalTemperature() --> 50
00:09:54.9	--- Oven temperatur: 50
00:09:54.9	--- Separator: Name: IonOpticks 25 cm, Func: Separator, ColDia: 0.075 mm, Len: 250 mm, PartDia: 1.6 µm, Porosity: 42 %
00:09:54.9	--- Spline fitting time: 97.6577595902957 s
00:09:54.9	--- Spline fitting time adapted: 10 s
00:09:54.9	--- y = 0x + 1
00:09:54.9	--- p0.Time -1, p0.Flow 1
00:09:54.9	--- p1.Time 0, p1.Flow 1
00:09:54.9	--- p2.Time 9, p2.Flow 1
00:09:54.9	--- p3.Time 10, p3.Flow 1, p3.Mix 0
00:09:54.9	--- p4.Time 40, p4.Flow 1, p4.Mix 0
00:09:54.9	--- Strategy function end
00:09:54.9	--- y = 0x + 0
00:09:54.9	--- p0.Time -1, p0.Flow 1
00:09:54.9	--- p1.Time 0, p1.Flow 1
00:09:54.9	--- p2.Time 9, p2.Flow 0
00:09:54.9	--- p3.Time 10, p3.Flow 0, p3.Mix 0
00:09:54.9	--- p4.Time 40, p4.Flow 0, p4.Mix 0
00:09:54.9	--- Strategy function end
00:09:54.9	xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx
00:09:54.9	XXXXX Time 0, Flow 2, Mix 50
00:09:54.9	XXXXX Time 1, Flow 1.94545, Mix 48.59813
00:09:55.0	XXXXX Time 2, Flow 1.84848, Mix 45.90163
00:09:55.0	XXXXX Time 3, Flow 1.72121, Mix 41.9014
00:09:55.0	XXXXX Time 4, Flow 1.57575, Mix 36.53846
00:09:55.0	XXXXX Time 5, Flow 1.42424, Mix 29.78723
00:09:55.0	XXXXX Time 6, Flow 1.27878, Mix 21.80094
00:09:55.0	XXXXX Time 7, Flow 1.15151, Mix 13.15789
00:09:55.0	XXXXX Time 8, Flow 1.05454, Mix 5.17241
00:09:55.0	XXXXX Time 9, Flow 1, Mix 0
00:09:55.0	--- Gradient is created, switching valves A and B
00:09:55.0	pump: GetSetManualValvePosition(A) --> 240
00:09:55.0	pump: GetCurrentPressure(A) --> 4.342344
00:09:55.0	pump: SetManualValvePosition(A, 240, False)
00:09:55.2	pump: GetSetManualValvePosition(B) --> 240
00:09:55.2	pump: GetCurrentPressure(B) --> 0
00:09:55.2	pump: SetManualValvePosition(B, 240, False)
00:09:55.4	--- Valves A and B switched to MixTee, starting gradient
00:09:55.4	--- LoadGradient() ---
00:09:55.4	--- Loading gradient method index: 0
00:09:55.4	pump: LoadMethod(0, ZrData.ZrPumpMethod)
00:09:55.9	pump: GetMethodState(0) --> loaded
00:09:55.9	--- Set gradient start
00:09:55.9	--- StartGradient() ---
00:09:55.9	--- Starting gradient method index: 0
00:09:55.9	pump: SetMethodSignal(0, StartTrunk)
00:09:56.1	pump: GetMethodState(0) --> runningTrunk
00:09:56.1	--- Start GradientSplineFit
00:09:56.1	pump: GetMethodState(0) --> runningTrunk
00:09:57.1	pump: GetCurrentPressure(A) --> 84.34638
00:09:57.1	pump: GetCurrentPressure(B) --> 44.93005
00:09:57.1	--- GradientSplineFit is running
00:10:05.1	--- GradientSplineFit has finished
00:10:05.1	--- End GradientSplineFit
00:10:05.1	--- Finished spline flow reduction B ---
00:10:06.2	pump: GetSetExternalTemperature() --> 50
00:10:06.2	--- Oven temperatur: 50
00:10:06.2	--- Separator: Name: IonOpticks 25 cm, Func: Separator, ColDia: 0.075 mm, Len: 250 mm, PartDia: 1.6 µm, Porosity: 42 %
00:10:06.2	--- Spline fitting time: 97.6577595902957 s
00:10:06.2	--- Spline fitting time adapted: 60 s
00:10:06.2	--- y = 0x + 0.01
00:10:06.2	--- p0.Time -1, p0.Flow 1
00:10:06.2	--- p1.Time 0, p1.Flow 1
00:10:06.2	--- p2.Time 59, p2.Flow 0.01
00:10:06.2	--- p3.Time 60, p3.Flow 0.01, p3.Mix 0
00:10:06.2	--- p4.Time 90, p4.Flow 0.01, p4.Mix 0
00:10:06.2	--- Strategy function end
00:10:06.2	--- y = 0x + 0
00:10:06.2	--- p0.Time -1, p0.Flow 0
00:10:06.2	--- p1.Time 0, p1.Flow 0
00:10:06.2	--- p2.Time 59, p2.Flow 0
00:10:06.2	--- p3.Time 60, p3.Flow 0, p3.Mix 0
00:10:06.2	--- p4.Time 90, p4.Flow 0, p4.Mix 0
00:10:06.2	--- Strategy function end
00:10:06.2	xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx
00:10:06.2	XXXXX Time 0, Flow 1, Mix 0
00:10:06.2	XXXXX Time 1, Flow 0.99837, Mix 0
00:10:06.2	XXXXX Time 2, Flow 0.99518, Mix 0
00:10:06.2	XXXXX Time 3, Flow 0.99048, Mix 0
00:10:06.2	XXXXX Time 4, Flow 0.98432, Mix 0
00:10:06.2	XXXXX Time 5, Flow 0.97675, Mix 0
00:10:06.2	XXXXX Time 6, Flow 0.96784, Mix 0
00:10:06.2	XXXXX Time 7, Flow 0.95763, Mix 0
00:10:06.2	XXXXX Time 8, Flow 0.94619, Mix 0
00:10:06.2	XXXXX Time 9, Flow 0.93356, Mix 0
00:10:06.3	XXXXX Time 10, Flow 0.91981, Mix 0
00:10:06.3	XXXXX Time 11, Flow 0.90498, Mix 0
00:10:06.3	XXXXX Time 12, Flow 0.88914, Mix 0
00:10:06.3	XXXXX Time 13, Flow 0.87233, Mix 0
00:10:06.3	XXXXX Time 14, Flow 0.85462, Mix 0
00:10:06.3	XXXXX Time 15, Flow 0.83605, Mix 0
00:10:06.3	XXXXX Time 16, Flow 0.81668, Mix 0
00:10:06.3	XXXXX Time 17, Flow 0.79658, Mix 0
00:10:06.3	XXXXX Time 18, Flow 0.77578, Mix 0
00:10:06.3	XXXXX Time 19, Flow 0.75435, Mix 0
00:10:06.3	XXXXX Time 20, Flow 0.73235, Mix 0
00:10:06.3	XXXXX Time 21, Flow 0.70982, Mix 0
00:10:06.3	XXXXX Time 22, Flow 0.68682, Mix 0
00:10:06.3	XXXXX Time 23, Flow 0.66341, Mix 0
00:10:06.3	XXXXX Time 24, Flow 0.63964, Mix 0
00:10:06.3	XXXXX Time 25, Flow 0.61558, Mix 0
00:10:06.4	XXXXX Time 26, Flow 0.59126, Mix 0
00:10:06.4	XXXXX Time 27, Flow 0.56675, Mix 0
00:10:06.4	XXXXX Time 28, Flow 0.5421, Mix 0
00:10:06.4	XXXXX Time 29, Flow 0.51737, Mix 0
00:10:06.4	XXXXX Time 30, Flow 0.49262, Mix 0
00:10:06.4	XXXXX Time 31, Flow 0.46789, Mix 0
00:10:06.4	XXXXX Time 32, Flow 0.44324, Mix 0
00:10:06.4	XXXXX Time 33, Flow 0.41873, Mix 0
00:10:06.4	XXXXX Time 34, Flow 0.39441, Mix 0
00:10:06.4	XXXXX Time 35, Flow 0.37035, Mix 0
00:10:06.4	XXXXX Time 36, Flow 0.34658, Mix 0
00:10:06.4	XXXXX Time 37, Flow 0.32317, Mix 0
00:10:06.4	XXXXX Time 38, Flow 0.30017, Mix 0
00:10:06.4	XXXXX Time 39, Flow 0.27764, Mix 0
00:10:06.4	XXXXX Time 40, Flow 0.25564, Mix 0
00:10:06.4	XXXXX Time 41, Flow 0.23421, Mix 0
00:10:06.4	XXXXX Time 42, Flow 0.21341, Mix 0
00:10:06.5	XXXXX Time 43, Flow 0.19331, Mix 0
00:10:06.5	XXXXX Time 44, Flow 0.17394, Mix 0
00:10:06.5	XXXXX Time 45, Flow 0.15537, Mix 0
00:10:06.5	XXXXX Time 46, Flow 0.13766, Mix 0
00:10:06.5	XXXXX Time 47, Flow 0.12085, Mix 0
00:10:06.5	XXXXX Time 48, Flow 0.10501, Mix 0
00:10:06.5	XXXXX Time 49, Flow 0.09018, Mix 0
00:10:06.5	XXXXX Time 50, Flow 0.07643, Mix 0
00:10:06.5	XXXXX Time 51, Flow 0.0638, Mix 0
00:10:06.5	XXXXX Time 52, Flow 0.05236, Mix 0
00:10:06.5	XXXXX Time 53, Flow 0.04215, Mix 0
00:10:06.5	XXXXX Time 54, Flow 0.03324, Mix 0
00:10:06.5	XXXXX Time 55, Flow 0.02567, Mix 0
00:10:06.5	XXXXX Time 56, Flow 0.01951, Mix 0
00:10:06.5	XXXXX Time 57, Flow 0.01481, Mix 0
00:10:06.5	XXXXX Time 58, Flow 0.01162, Mix 0
00:10:06.6	XXXXX Time 59, Flow 0.00999, Mix 0
00:10:06.6	--- Gradient is created, switching valves A and B
00:10:06.6	pump: GetSetManualValvePosition(A) --> 240
00:10:06.6	pump: GetCurrentPressure(A) --> 89.00031
00:10:06.6	pump: SetManualValvePosition(A, 240, False)
00:10:06.8	pump: GetSetManualValvePosition(B) --> 240
00:10:06.8	pump: GetCurrentPressure(B) --> 12.07653
00:10:06.8	pump: SetManualValvePosition(B, 240, False)
00:10:07.0	--- Valves A and B switched to MixTee, starting gradient
00:10:07.0	--- LoadGradient() ---
00:10:07.0	--- Loading gradient method index: 0
00:10:07.0	pump: LoadMethod(0, ZrData.ZrPumpMethod)
00:10:07.5	pump: GetMethodState(0) --> loaded
00:10:07.5	--- Set gradient start
00:10:07.5	--- StartGradient() ---
00:10:07.5	--- Starting gradient method index: 0
00:10:07.5	pump: SetMethodSignal(0, StartTrunk)
00:10:07.7	pump: GetMethodState(0) --> runningTrunk
00:10:07.7	--- Start GradientSplineFit
00:10:07.7	pump: GetMethodState(0) --> runningTrunk
00:10:08.7	pump: GetCurrentPressure(A) --> 90.14406
00:10:08.7	pump: GetCurrentPressure(B) --> 13.89077
00:10:08.7	--- GradientSplineFit is running
00:11:06.7	--- GradientSplineFit has finished
00:11:06.7	--- End GradientSplineFit
00:11:06.7	--- Finished spline flow reduction A ---

00:11:06.7	--- Finished conditioning paths ---
00:11:12.3	autosampler-none: MoveValveDrive(Valve Drive 2, 240 deg)
00:11:12.3	pump: GetSetExternalTemperature() --> 50
00:11:12.3	--- Oven temperatur: 50
00:11:12.3	--- Separator: Name: IonOpticks 25 cm, Func: Separator, ColDia: 0.075 mm, Len: 250 mm, PartDia: 1.6 µm, Porosity: 42 %
00:11:12.3	--- Spline fitting time: 97.6577595902957 s
00:11:12.3	--- Spline fitting time adapted: 53.7117677746626 s
00:11:12.3	--- Press: 541.893115083548; colPress: 511.893115083548, visco: 0.00566684100509561, flow > 0.3
00:11:12.3	--- Flow: 0.3; Mix: 0, calcI: 220.674819441516
00:11:12.3	--- Ki calc: 220.674819441516
00:11:12.3	pump: GetSettings() --> PumpSettings
00:11:12.3	------------------------------------------------------------------
00:11:12.3	PID settings changed:
00:11:12.3	------------------------------------------------------------------
00:11:12.3	P: 200 : True; Pmin: -32000 : True; Pmax: 32000 : True
00:11:12.3	I: 600 : True; Imin: -32000 : True; Imax: 32000 : True; IWindow: 100 : True
00:11:12.3	D: 1000 : True; Dmin: -32000 : True; Dmax: 32000 : True
00:11:12.3	Result min: -32000 : True; Result max: 32000 : True
00:11:12.3	------------------------------------------------------------------
00:11:12.3	pump: SetSettings(PumpSettings)
00:11:12.3	pump: GetSettings() --> PumpSettings
00:11:12.3	------------------------------------------------------------------
00:11:12.3	PID settings changed:
00:11:12.3	------------------------------------------------------------------
00:11:12.3	P: 200 : False; Pmin: -32000 : False; Pmax: 32000 : False
00:11:12.3	I: 220 : True; Imin: -32000 : False; Imax: 32000 : False; IWindow: 100 : False
00:11:12.3	D: 1000 : False; Dmin: -32000 : False; Dmax: 32000 : False
00:11:12.3	Result min: -32000 : False; Result max: 32000 : False
00:11:12.3	------------------------------------------------------------------
00:11:12.3	pump: SetSettings(PumpSettings)
00:11:12.3	--- y = -5.33333333333333E-05x + 0.302864627614649
00:11:12.3	--- p0.Time -1, p0.Flow 0.01
00:11:12.3	--- p1.Time 0, p1.Flow 0.01
00:11:12.3	--- p2.Time 52.7117677746626, p2.Flow 0.300053333333333
00:11:12.3	--- p3.Time 53.7117677746626, p3.Flow 0.3, p3.Mix 0
00:11:12.3	--- p4.Time 1853.71176777466, p4.Flow 0.204, p4.Mix 32
00:11:12.3	--- Strategy function end
00:11:12.3	--- y = 5.33333333333333E-05x + -0.00286462761464867
00:11:12.3	--- p0.Time -1, p0.Flow 0
00:11:12.3	--- p1.Time 0, p1.Flow 0
00:11:12.3	--- p2.Time 52.7117677746626, p2.Flow -5.33333333333336E-05
00:11:12.3	--- p3.Time 53.7117677746626, p3.Flow 0, p3.Mix 0
00:11:12.3	--- p4.Time 1853.71176777466, p4.Flow 0.096, p4.Mix 32
00:11:12.3	--- Strategy function end
00:11:12.3	xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx
00:11:12.4	XXXXX Time 0, Flow 0.01, Mix 0
00:11:12.4	XXXXX Time 1, Flow 0.01059, Mix 0
00:11:12.4	XXXXX Time 2, Flow 0.01175, Mix 0
00:11:12.4	XXXXX Time 3, Flow 0.01347, Mix 0
00:11:12.4	XXXXX Time 4, Flow 0.01571, Mix 0
00:11:12.4	XXXXX Time 5, Flow 0.01845, Mix 0
00:11:12.4	XXXXX Time 6, Flow 0.02168, Mix 0
00:11:12.4	XXXXX Time 7, Flow 0.02537, Mix 0
00:11:12.4	XXXXX Time 8, Flow 0.02949, Mix 0
00:11:12.4	XXXXX Time 9, Flow 0.03402, Mix 0
00:11:12.4	XXXXX Time 10, Flow 0.03894, Mix 0
00:11:12.4	XXXXX Time 11, Flow 0.04424, Mix 0
00:11:12.5	XXXXX Time 12, Flow 0.04988, Mix 0
00:11:12.5	XXXXX Time 13, Flow 0.05584, Mix 0
00:11:12.5	XXXXX Time 14, Flow 0.0621, Mix 0
00:11:12.5	XXXXX Time 15, Flow 0.06864, Mix 0
00:11:12.5	XXXXX Time 16, Flow 0.07544, Mix 0
00:11:12.5	XXXXX Time 17, Flow 0.08247, Mix 0
00:11:12.5	XXXXX Time 18, Flow 0.08971, Mix 0
00:11:12.5	XXXXX Time 19, Flow 0.09713, Mix 0
00:11:12.5	XXXXX Time 20, Flow 0.10472, Mix 0
00:11:12.5	XXXXX Time 21, Flow 0.11246, Mix 0
00:11:12.5	XXXXX Time 22, Flow 0.12031, Mix 0
00:11:12.5	XXXXX Time 23, Flow 0.12826, Mix 0
00:11:12.5	XXXXX Time 24, Flow 0.13629, Mix 0
00:11:12.5	XXXXX Time 25, Flow 0.14437, Mix 0
00:11:12.5	XXXXX Time 26, Flow 0.15248, Mix 0
00:11:12.5	XXXXX Time 27, Flow 0.16059, Mix 0
00:11:12.5	XXXXX Time 28, Flow 0.16869, Mix 0
00:11:12.6	XXXXX Time 29, Flow 0.17675, Mix 0
00:11:12.6	XXXXX Time 30, Flow 0.18476, Mix 0
00:11:12.6	XXXXX Time 31, Flow 0.19267, Mix 0
00:11:12.6	XXXXX Time 32, Flow 0.20049, Mix 0
00:11:12.6	XXXXX Time 33, Flow 0.20817, Mix 0
00:11:12.6	XXXXX Time 34, Flow 0.2157, Mix 0
00:11:12.6	XXXXX Time 35, Flow 0.22306, Mix 0
00:11:12.6	XXXXX Time 36, Flow 0.23022, Mix 0
00:11:12.6	XXXXX Time 37, Flow 0.23717, Mix 0
00:11:12.6	XXXXX Time 38, Flow 0.24387, Mix 0
00:11:12.6	XXXXX Time 39, Flow 0.25031, Mix 0
00:11:12.6	XXXXX Time 40, Flow 0.25646, Mix 0
00:11:12.6	XXXXX Time 41, Flow 0.2623, Mix 0
00:11:12.6	XXXXX Time 42, Flow 0.26781, Mix 0
00:11:12.6	XXXXX Time 43, Flow 0.27297, Mix 0
00:11:12.6	XXXXX Time 44, Flow 0.27775, Mix 0
00:11:12.6	XXXXX Time 45, Flow 0.28213, Mix 0
00:11:12.7	XXXXX Time 46, Flow 0.28609, Mix 0
00:11:12.7	XXXXX Time 47, Flow 0.28961, Mix 0
00:11:12.7	XXXXX Time 48, Flow 0.29265, Mix 0
00:11:12.7	XXXXX Time 49, Flow 0.29521, Mix 0
00:11:12.7	XXXXX Time 50, Flow 0.29726, Mix 0
00:11:12.7	XXXXX Time 51, Flow 0.29877, Mix 0
00:11:12.7	XXXXX Time 52, Flow 0.29972, Mix 0
00:11:12.7	XXXXX Time 53, Flow 0.3001, Mix 0
00:11:12.7	--- Gradient is created, switching valves A and B
00:11:12.7	pump: GetSetManualValvePosition(A) --> 240
00:11:12.7	pump: GetCurrentPressure(A) --> 1.127985
00:11:12.7	pump: SetManualValvePosition(A, 240, False)
00:11:12.9	pump: GetSetManualValvePosition(B) --> 240
00:11:12.9	pump: GetCurrentPressure(B) --> 0
00:11:12.9	pump: SetManualValvePosition(B, 240, False)
00:11:13.1	--- Valves A and B switched to MixTee, starting gradient
00:11:13.1	--- LoadGradient() ---
00:11:13.1	--- Loading gradient method index: 0
00:11:13.1	pump: LoadMethod(0, ZrData.ZrPumpMethod)
00:11:13.6	pump: GetMethodState(0) --> loaded
00:11:13.6	--- Set gradient start
00:11:13.6	--- StartGradient() ---
00:11:13.6	--- Starting gradient method index: 0
00:11:13.6	pump: SetMethodSignal(0, StartTrunk)
00:11:13.8	pump: GetMethodState(0) --> runningTrunk
00:11:13.8	--- Start GradientSplineFit
00:11:13.8	pump: GetMethodState(0) --> runningTrunk
00:11:14.8	pump: GetCurrentPressure(A) --> 0
00:11:14.8	pump: GetCurrentPressure(B) --> 0
00:11:14.8	--- GradientSplineFit is running
00:12:06.8	--- GradientSplineFit has finished
00:12:06.8	--- End GradientSplineFit
00:12:06.8	status: RemoveStatus(preparing gradient start)
00:12:06.9	Setting signal "lc-ready"
00:12:06.9	Waiting for signal "ms-start"...
00:12:06.9	--- LoadGradient() ---
00:12:06.9	--- Loading gradient method index: 0
00:12:06.9	pump: LoadMethod(0, ZrData.ZrPumpMethod)
00:12:07.4	pump: GetMethodState(0) --> loaded
00:12:07.4	--- Set gradient start
00:12:07.4	--- StartGradient() ---
00:12:07.4	--- Starting gradient method index: 0
00:12:07.4	pump: SetMethodSignal(0, StartTrunk)
00:12:07.6	pump: GetMethodState(0) --> runningTrunk
00:12:08.4	autosampler-none: SetSignal(Output Signal 1)
00:12:08.4	status: SetStatus(wash, )
00:12:12.3	status: SetStatus(elution, )
00:12:14.1	autosampler-left: MoveToObject(LCInjector1)
00:12:16.8	autosampler-left: PenetrateWithConstForce(LCInjector1)
00:12:16.9	autosampler-left: MoveValveDrive(Valve Drive 1, 0 deg)
00:12:46.3	autosampler-left: CleanInjector(LCInjector1, LCP 1, 100 uL, 0 mm, 5 uL/s, 5 s, 20 mm, 40 uL/s, 1 s, 2, False)
00:14:16.9	autosampler-left: CleanInjector(LCInjector1, LCP 1, 300 uL, 0 mm, 5 uL/s, 5 s, 20 mm, 40 uL/s, 1 s, 1, True)
00:14:17.1	autosampler-left: MoveValveDrive(Valve Drive 1, 60 deg)
00:14:20.1	autosampler-left: MoveToHome()
01:12:08.5	Setting signal "lc-done"
01:12:08.5	status: RemoveStatus(elution)
01:12:08.5	status: RemoveStatus(wash)
01:12:08.5	pump: GetCurrentPressure(A) --> 233.0746
01:12:08.5	pump: GetCurrentPressure(B) --> 241.1835
01:12:08.5	pump: GetSettings() --> PumpSettings
01:12:08.5	pump: GetSetManualValvePosition(A) --> 240
01:12:08.5	pump: GetSetManualValvePosition(B) --> 240
01:12:08.5	-------------------------------------------------------
01:12:08.5	--- Reduce pressure ---
01:12:08.5	-------------------------------------------------------
01:12:08.5	--- Pressure channel A: 233.074615478516
01:12:08.5	--- Pressure channel B: 241.183471679688
01:12:08.5	pump: GetSettings() --> PumpSettings
01:12:08.5	------------------------------------------------------------------
01:12:08.5	PID settings changed:
01:12:08.5	------------------------------------------------------------------
01:12:08.5	P: 250 : True; Pmin: -5500 : True; Pmax: 32000 : True
01:12:08.5	I: 50 : True; Imin: -20000 : True; Imax: 20000 : True; IWindow: 50 : True
01:12:08.5	D: 1500 : True; Dmin: -10000 : True; Dmax: 10000 : True
01:12:08.5	Result min: -32000 : True; Result max: 32000 : True
01:12:08.5	------------------------------------------------------------------
01:12:08.5	pump: SetSettings(PumpSettings)
01:12:08.7	--- Set pressure channel A:          10
01:12:08.7	--- Set pressure channel B:          5
01:12:08.7	--- Reduce pressure flow controlled: True
01:12:39.0	--------------------------------------------------------
01:12:39.0	--- Log PID parameter called from: reducePressure
01:12:39.0	--------------------------------------------------------
01:12:39.0	--- P: 200; Pmin: -32000; Pmax: 32000
01:12:39.0	--- I: 220; Imin: -32000; Imax: 32000; IWindow: 100
01:12:39.0	--- D: 1000; Dmin: -32000; Dmax: 32000
01:12:39.0	--- Result min: -32000; Result max: 32000
01:12:39.0	--------------------------------------------------------
01:12:39.0	--------------------------------------------------------
01:12:39.0	--- Log instrument settings called from: reducePressure
01:12:39.0	--------------------------------------------------------
01:12:39.0	--- HomeSpeed: 2000; PurgeSpeed: 2000
01:12:39.0	--- PressureTrackingExcessFactor: 96
01:12:39.0	--- FlowResistanceA: 300; FlowResistanceB: 100; FlowResistanceOut: 150
01:12:39.0	--- FC_FlowFilterDepth: 5; FC_PressureFilterDepth: 0
01:12:39.0	--- PC_OutputFilterDepth: 0
01:12:39.0	--- CompressibilityFactorB: 2.29999995231628; PressurePIDNegativeResultDivisor: 1
01:12:39.0	--- ExternalZeroPressureOffsetA: 130.700210571289; ExternalZeroPressureOffsetB: 127.99462890625
01:12:39.0	--- ExternalPressureScalingFactorA: 0.0197199992835522; ExternalPressureScalingFactorB: 0.0197199992835522
01:12:39.0	--------------------------------------------------------
01:12:39.0	--- Final pressure channel A: 5.38750457763672
01:12:39.0	--- Final pressure channel B: 3.8532862663269
01:12:39.0	-------------------------------------------------------
01:12:39.3	autosampler-left: MoveValveDrive(Valve Drive 2, 0 deg)
01:12:39.5	pump: Manualmode_Pump_constantSpeed(A, 0)
01:12:39.5	pump: GetSetPistonSpeed(A) --> 0
01:12:39.5	pump: Manualmode_Pump_constantSpeed(B, 0)
01:12:39.5	pump: GetSetPistonSpeed(B) --> 0
01:12:39.5	pump: IsIdle() --> True
01:12:39.6	autosampler-left: MoveValveDrive(Valve Drive 1, 60 deg)
01:12:41.8	autosampler-left: MoveToHome()
01:12:41.8	pump: IsIdle() --> True
01:12:41.8	COMPLETED