Path: 2022-07-30_13-27-42_Two-column-separation
Procedure: Two column separation
Argument list:
uses_trap = True
uses_separator = True
is_isocratic = False
analysis_time = 1800
oven_temperature = 50
sample_volume = 5
sample_position = Slot2:12
presample_solvent_volume = 2
presample_air_volume = 0.5
postsample_air_volume = 1
postsample_solvent_volume = 2
sample_aspirate_speed = 5
sample_postaspirate_delay = 500
sample_inject_speed = 1
calibrant_volume = 0.6
calibrantTime = 10
is_calibrant_inject = False
is_extended_wash = False
is_bottom_sense = True
injection_method = uLPickup
trap = Name: Thermo Trap Cartridge 5mm, Func: Separator, ColDia: 0.3 mm, Len: 5 mm, PartDia: 5 µm, Porosity: 42 %
trap_volume = 0.148440252882118
trap_unityflow = 2.78838695282287
trap_equilibration_volumemultiplier = 10
trap_equilibration_pressure = 217.5
trap_equil_time = 0.648440252882118
separator = Name: IonOpticks 25 cm, Func: Separator, ColDia: 0.075 mm, Len: 250 mm, PartDia: 1.6 µm, Porosity: 42 %
separator_volume = 0.463875790256618
separator_unityflow = 0.00058605984562038
separator_equilibration_volumemultiplier = 4
separator_equilibration_pressure = 800
column_load_volumemultiplier = 4
column_load_pressure = 217.5
separator_equil_time = 4.05402026133927
column_load_time = 0.6
gradient = (0 sec,0.3 µL/min,0.00 %), (1800 sec,0.3 µL/min,32.00 %), (2700 sec,0.3 µL/min,40.00 %), (3000 sec,0.3 µL/min,60.00 %), (3300 sec,0.3 µL/min,95.00 %), (3600 sec,0.3 µL/min,95.00 %)
00:00:00.0	STARTED
00:00:00.3	Lua version:		1.1.0
00:00:00.3	Lua date:			2020/01/30
00:00:00.3	--- Experiment:    Description
00:00:00.3	-------------------------------------------------------
00:00:00.3	--- Trap Column:              Thermo Trap Cartridge 5mm
00:00:00.3	---    Manufacturer:          Thermo Scientific
00:00:00.3	---    Part Number:           
00:00:00.3	---    Serial Number:         
00:00:00.3	---    Type/Material:         µ-Precolum TM Cartridge  / PepMap TM C18
00:00:00.3	---    Length:                5 mm
00:00:00.3	---    Inner Diameter:        0.3 mm
00:00:00.3	---    Particle Diameter:     5 um
00:00:00.3	---    Is Particle Pore Size: True
00:00:00.3	---    Particle Pore Size:    100
00:00:00.3	---    Is Column Porosity:    False
00:00:00.3	---    Column Porosity:       0.42 (0.0-1.0)
00:00:00.3	---    Is Max Temperature:    False
00:00:00.3	---    max Temperature:       65 C
00:00:00.3	---    Is Max Pressure:       False
00:00:00.3	---    max Pressure:          1000 bar
00:00:00.3	---    Is Max Flow:           False
00:00:00.3	---    max Flow:              9 uL/min
00:00:00.3	---    Advanced Settings:     False
00:00:00.3	---    Column Comments:       Manufacturer no: 160454
00:00:00.3	-------------------------------------------------------
00:00:00.3	--- Separation Column:        IonOpticks 25 cm
00:00:00.3	---    Manufacturer:          IonOpticks
00:00:00.3	---    Part Number:           
00:00:00.3	---    Serial Number:         
00:00:00.3	---    Type/Material:         C18
00:00:00.3	---    Length:                250 mm
00:00:00.3	---    Inner Diameter:        0.075 mm
00:00:00.3	---    Particle Diameter:     1.6 um
00:00:00.3	---    Is Particle Pore Size: True
00:00:00.3	---    Particle Pore Size:    120
00:00:00.3	---    Is Column Porosity:    False
00:00:00.3	---    Column Porosity:       0.42 (0.0-1.0)
00:00:00.3	---    Is Max Temperature:    False
00:00:00.3	---    max Temperature:       65 C
00:00:00.3	---    Is Max Pressure:       True
00:00:00.3	---    max Pressure:          1000 bar
00:00:00.3	---    Is Max Flow:           False
00:00:00.3	---    max Flow:              9 uL/min
00:00:00.3	---    Advanced Settings:     False
00:00:00.3	---    Column Comments:           
00:00:00.3	-------------------------------------------------------
00:00:00.3	'Valve Drive 1' is 'ILcInjectorValve'
00:00:00.3	'Valve Drive 2' is 'ISelectorValve'
00:00:00.3	--- Trap equil time:		0.64
00:00:00.3	--- Seperator equil time:	4.05
00:00:00.3	--- Column loading time:	0.6
00:00:00.3	pump: GetSettings() --> PumpSettings
00:00:00.3	--------------------------------------------------------
00:00:00.3	--- Log instrument settings called from: Two Column Separation
00:00:00.3	--------------------------------------------------------
00:00:00.3	--- HomeSpeed: 2000; PurgeSpeed: 2000
00:00:00.3	--- PressureTrackingExcessFactor: 96
00:00:00.3	--- FlowResistanceA: 300; FlowResistanceB: 100; FlowResistanceOut: 150
00:00:00.3	--- FC_FlowFilterDepth: 5; FC_PressureFilterDepth: 0
00:00:00.3	--- PC_OutputFilterDepth: 0
00:00:00.3	--- CompressibilityFactorB: 2.29999995231628; PressurePIDNegativeResultDivisor: 1
00:00:00.3	--- ExternalZeroPressureOffsetA: 130.944732666016; ExternalZeroPressureOffsetB: 128.061676025391
00:00:00.3	--- ExternalPressureScalingFactorA: 0.0197199992835522; ExternalPressureScalingFactorB: 0.0197199992835522
00:00:00.3	--------------------------------------------------------
00:00:00.3	--- Oven temperature: 50
00:00:00.3	--- Separator: Name: IonOpticks 25 cm, Func: Separator, ColDia: 0.075 mm, Len: 250 mm, PartDia: 1.6 µm, Porosity: 42 % ---
00:00:00.3	--- Trap: Name: Thermo Trap Cartridge 5mm, Func: Separator, ColDia: 0.3 mm, Len: 5 mm, PartDia: 5 µm, Porosity: 42 % ---
00:00:00.3	--- MaxPressureLimitAB: 1000 bar ---
00:00:00.3	pump: GetCurrentPressure(A) --> 6.247303
00:00:00.3	pump: GetCurrentPressure(B) --> 6.073759
00:00:00.3	pump: GetSettings() --> PumpSettings
00:00:00.3	pump: GetSetManualValvePosition(A) --> 240
00:00:00.3	pump: GetSetManualValvePosition(B) --> 240
00:00:00.3	-------------------------------------------------------
00:00:00.3	--- Reduce pressure ---
00:00:00.3	-------------------------------------------------------
00:00:00.3	--- Pressure channel A: 6.24730253219604
00:00:00.3	--- Pressure channel B: 6.07375907897949
00:00:00.3	pump: Manualmode_Pump_constantSpeed(A, 0)
00:00:00.3	pump: GetSetPistonSpeed(A) --> 0
00:00:00.3	pump: Manualmode_Pump_constantSpeed(B, 0)
00:00:00.3	pump: GetSetPistonSpeed(B) --> 0
00:00:00.3	pump: IsIdle() --> True
00:00:00.3	-------------------------------------------------------
00:00:00.4	pump: SetMaxPressureLimit(A, 1020)
00:00:00.4	pump: GetMaxPressureLimit(A) --> 1020
00:00:00.5	pump: SetMaxPressureLimit(B, 1020)
00:00:00.5	pump: GetMaxPressureLimit(B) --> 1020
00:00:00.5	pump: GetSettings() --> PumpSettings
00:00:00.5	------------------------------------------------------------------
00:00:00.5	PID settings changed:
00:00:00.5	------------------------------------------------------------------
00:00:00.5	P: 175 : True; Pmin: -5000 : True; Pmax: 5000 : True
00:00:00.5	I: 5 : True; Imin: -200 : True; Imax: 1000 : True; IWindow: 10 : True
00:00:00.5	D: 0 : True; Dmin: -1000 : True; Dmax: 1000 : True
00:00:00.5	Result min: -6000 : True; Result max: 8000 : True
00:00:00.5	------------------------------------------------------------------
00:00:00.5	pump: SetSettings(PumpSettings)
00:00:00.5	pump: GetPumpSideState(A) --> manualMode
00:00:00.5	pump: GetPumpSideState(B) --> manualMode
00:00:00.5	pump: GetPistonPosition(A) --> 447.948596181326
00:00:00.5	pump: GetPistonPosition(B) --> 90.3283286130752
00:00:00.5	-------------------------------------------------------
00:00:00.5	--- Optional Refill of Pump Heads:
00:00:00.5	--- initiated by channel A:     False, B: False
00:00:00.5	pump: GetPistonPosition(A) --> 447.948596181326
00:00:00.5	pump: GetPistonPosition(B) --> 90.3283286130752
00:00:00.5	--- Piston position channel A:  447.948596181326, B: 90.3283286130752
00:00:00.5	-------------------------------------------------------
00:00:00.5	pump: SetDigitalOutput(PO2, False)
00:00:00.5	pump: GetCurrentPressure(A) --> 6.405062
00:00:00.5	pump: GetCurrentPressure(B) --> 5.975159
00:00:00.5	pump: GetSettings() --> PumpSettings
00:00:00.5	pump: GetSetManualValvePosition(A) --> 240
00:00:00.5	pump: GetSetManualValvePosition(B) --> 240
00:00:00.5	-------------------------------------------------------
00:00:00.5	--- Reduce pressure ---
00:00:00.5	-------------------------------------------------------
00:00:00.5	--- Pressure channel A: 6.40506219863892
00:00:00.5	--- Pressure channel B: 5.97515916824341
00:00:00.5	pump: GetSettings() --> PumpSettings
00:00:00.5	------------------------------------------------------------------
00:00:00.5	PID settings changed:
00:00:00.5	------------------------------------------------------------------
00:00:00.5	P: 250 : True; Pmin: -5500 : True; Pmax: 32000 : True
00:00:00.5	I: 50 : True; Imin: -20000 : True; Imax: 20000 : True; IWindow: 50 : True
00:00:00.5	D: 1500 : True; Dmin: -10000 : True; Dmax: 10000 : True
00:00:00.5	Result min: -32000 : True; Result max: 32000 : True
00:00:00.5	------------------------------------------------------------------
00:00:00.5	pump: SetSettings(PumpSettings)
00:00:00.7	--- Set pressure channel A:          10
00:00:00.7	--- Set pressure channel B:          5
00:00:00.7	--- Reduce pressure flow controlled: True
00:00:01.8	--------------------------------------------------------
00:00:01.8	--- Log PID parameter called from: reducePressure
00:00:01.8	--------------------------------------------------------
00:00:01.8	--- P: 175; Pmin: -5000; Pmax: 5000
00:00:01.8	--- I: 5; Imin: -200; Imax: 1000; IWindow: 10
00:00:01.8	--- D: 0; Dmin: -1000; Dmax: 1000
00:00:01.8	--- Result min: -6000; Result max: 8000
00:00:01.8	--------------------------------------------------------
00:00:01.8	--------------------------------------------------------
00:00:01.8	--- Log instrument settings called from: reducePressure
00:00:01.8	--------------------------------------------------------
00:00:01.8	--- HomeSpeed: 2000; PurgeSpeed: 2000
00:00:01.8	--- PressureTrackingExcessFactor: 96
00:00:01.8	--- FlowResistanceA: 300; FlowResistanceB: 100; FlowResistanceOut: 150
00:00:01.8	--- FC_FlowFilterDepth: 5; FC_PressureFilterDepth: 0
00:00:01.8	--- PC_OutputFilterDepth: 0
00:00:01.8	--- CompressibilityFactorB: 2.29999995231628; PressurePIDNegativeResultDivisor: 1
00:00:01.8	--- ExternalZeroPressureOffsetA: 130.944732666016; ExternalZeroPressureOffsetB: 128.061676025391
00:00:01.8	--- ExternalPressureScalingFactorA: 0.0197199992835522; ExternalPressureScalingFactorB: 0.0197199992835522
00:00:01.8	--------------------------------------------------------
00:00:01.8	--- Final pressure channel A: 6.38534212112427
00:00:01.8	--- Final pressure channel B: 0
00:00:01.8	-------------------------------------------------------
00:00:01.8	pump: GetSetManualValvePosition(A) --> 240
00:00:01.8	pump: GetCurrentPressure(A) --> 6.385342
00:00:01.8	pump: SetManualValvePosition(A, 0, True)
00:00:02.4	pump: GetSetManualValvePosition(B) --> 240
00:00:02.4	pump: GetCurrentPressure(B) --> 0
00:00:02.4	pump: SetManualValvePosition(B, 180, False)
00:00:02.8	pump: GetSettings() --> PumpSettings
00:00:02.8	------------------------------------------------------------------
00:00:02.8	PID settings changed:
00:00:02.8	------------------------------------------------------------------
00:00:02.8	P: 250 : True; Pmin: -10000 : True; Pmax: 10000 : True
00:00:02.8	I: 20 : True; Imin: -10000 : True; Imax: 20000 : True; IWindow: 10 : True
00:00:02.8	D: 1500 : True; Dmin: -10000 : True; Dmax: 10000 : True
00:00:02.8	Result min: -10000 : True; Result max: 32000 : True
00:00:02.8	------------------------------------------------------------------
00:00:02.8	pump: SetSettings(PumpSettings)
00:00:02.8	status: SetStatus(equilibrate separation-column, this finishes at about: 4.84 min., )
00:00:03.5	autosampler-none: MoveValveDrive(Valve Drive 2, 180 deg)
00:00:03.5	--- MaxPressureLimitA: 800 bar ---
00:00:03.5	pump: GetCurrentPressure(A) --> 6.385342
00:00:03.5	pump: GetCurrentPressure() --> 6.385342
00:00:03.5	pump: GetSettings() --> PumpSettings
00:00:03.5	pump: GetSetManualValvePosition(A) --> 0
00:00:03.5	-------------------------------------------------------
00:00:03.5	--- Reduce pressure ---
00:00:03.5	-------------------------------------------------------
00:00:03.5	--- Pressure channel A: 6.38534212112427
00:00:03.5	--- Pressure channel B: 6.38534212112427
00:00:03.5	pump: Manualmode_Pump_constantSpeed(A, 0)
00:00:03.5	pump: GetSetPistonSpeed(A) --> 0
00:00:03.5	pump: Manualmode_Pump_constantSpeed(, 0)
00:00:03.5	pump: GetSetPistonSpeed() --> 0
00:00:03.5	pump: IsIdle() --> True
00:00:03.5	-------------------------------------------------------
00:00:03.6	pump: SetMaxPressureLimit(A, 820)
00:00:03.6	pump: GetMaxPressureLimit(A) --> 820
00:00:03.6	-------------------------------------------------------
00:00:03.6	--- Separation Column Equilibration:
00:00:03.6	-------------------------------------------------------
00:00:03.6	--- ovenTemp [C]:                                 50
00:00:03.6	--- Separator Unity-Flow @ ovenTemp [ul/min/bar]: 0.00058605984562038
00:00:03.6	--- Separator Volume [ul]:                        0.463875790256618
00:00:03.6	--- Separator Equilibration Volume Multiplier:    4
00:00:03.6	--- Separator Equilibration Pressure [bar]:       800
00:00:03.6	--- Separator Equilibration Flow [ul/min]:        0.457694594849779
00:00:03.6	--- Separator Equilibration Volume [ul]:          1.85550316102647
00:00:03.6	--- Separator Equilibration Time [min]:           4.05402026133927
00:00:03.6	--- max. Volume (10*V+2ul/min*t) [ul]:            26.6630721329433
00:00:03.6	--- Separator Equilibration Time Out [min]:       40.5402026133927
00:00:03.6	-------------------------------------------------------
00:00:03.6	pump: Manualmode_Pump_constantPressure(A, 800)
00:00:03.6	pump: GetPistonPosition(A) --> 447.948596181326
00:00:03.6	--- Injection delay: 237.164855895902s
00:00:26.6	pump: GetPistonPosition(A) --> 522.76855737971
00:00:26.6	pump: GetPistonPosition(A) --> 522.76855737971
00:00:26.6	pump: GetPistonPosition(A) --> 522.76855737971
00:04:00.8	status: SetStatus(aspirate & inject, )
00:04:01.0	autosampler-left: LeaveObject()
00:04:05.0	autosampler-left: MoveToObject(LCMS Wash 1, 3)
00:04:06.0	autosampler-left: PenetrateObject(LCMS Wash 1, 3)
00:04:06.1	autosampler-left: EmptySyringe()
00:04:06.8	autosampler-left: LeaveObject()
00:04:06.8	Cleaning syringe and needle exterior
00:04:22.7	autosampler-left: CleanSyringe(LCMS Wash 1, 1, 30 mm, 40 uL/s, 500 ms, 30 mm, 40 uL/s, 80 %, 2)
00:04:22.7	autosampler-left: ChangeTool(LCP 1) --> False
00:04:25.9	autosampler-left: MoveToObject(Item Position 3, 1, True, True, True)
00:04:29.0	autosampler-left: PenetrateWithBottomSense(Item Position 3, 1, 1 mm)
00:04:30.4	autosampler-left: AspirateSyringe(2 uL, 5 uL/s, 500 ms)
00:04:30.5	pump: GetPistonPosition(A) --> 526.183887473183
00:04:30.5	pump: GetCurrentPressure(A) --> 799.9378
00:04:30.5	--- act. pressure [bar]:                 799.937805175781
00:04:30.5	--- used volume [uL]:                    3.41533009347256
00:04:30.5	--- average flow (incl. leaks) [uL/min]: 0.840127936406199
00:04:30.5	--- act. equilibration time [min]:       4.06524999999674
00:04:30.5	-------------------------------------------------------
00:04:30.5	status: RemoveStatus(equilibrate separation-column, this finishes at about: 4.84 min.)
00:04:30.5	status: SetStatus(equilibrate trap-column, this finishes at about: 5.65 min., )
00:04:30.6	--- MaxPressureLimitA: 217.5 bar ---
00:04:30.6	pump: GetCurrentPressure(A) --> 799.9378
00:04:30.6	pump: GetCurrentPressure() --> 799.9378
00:04:30.6	pump: GetSettings() --> PumpSettings
00:04:30.6	pump: GetSetManualValvePosition(A) --> 0
00:04:30.6	-------------------------------------------------------
00:04:30.6	--- Reduce pressure ---
00:04:30.6	-------------------------------------------------------
00:04:30.6	--- Pressure channel A: 799.937805175781
00:04:30.6	--- Pressure channel B: 799.937805175781
00:04:30.6	pump: GetSettings() --> PumpSettings
00:04:30.6	------------------------------------------------------------------
00:04:30.6	PID settings changed:
00:04:30.6	------------------------------------------------------------------
00:04:30.6	P: 250 : True; Pmin: -32000 : True; Pmax: 32000 : True
00:04:30.6	I: 20 : True; Imin: -20000 : True; Imax: 20000 : True; IWindow: 100 : True
00:04:30.6	D: 1500 : True; Dmin: -10000 : True; Dmax: 10000 : True
00:04:30.6	Result min: -32000 : True; Result max: 32000 : True
00:04:30.6	------------------------------------------------------------------
00:04:30.6	pump: SetSettings(PumpSettings)
00:04:30.7	--- Set pressure channel A:          217.5
00:04:30.7	--- Set pressure channel B:          217.5
00:04:30.7	--- Reduce pressure flow controlled: False
00:04:31.2	autosampler-left: LeaveObject()
00:04:31.9	autosampler-left: AspirateSyringe(0.5 uL, 5 uL/s, 500 ms)
00:04:42.7	autosampler-left: MoveToObject(Rack 2, 12, True, True, True)
00:04:44.9	--------------------------------------------------------
00:04:44.9	--- Log PID parameter called from: reducePressure
00:04:44.9	--------------------------------------------------------
00:04:44.9	--- P: 250; Pmin: -10000; Pmax: 10000
00:04:44.9	--- I: 20; Imin: -10000; Imax: 20000; IWindow: 10
00:04:44.9	--- D: 1500; Dmin: -10000; Dmax: 10000
00:04:44.9	--- Result min: -10000; Result max: 32000
00:04:44.9	--------------------------------------------------------
00:04:44.9	--------------------------------------------------------
00:04:44.9	--- Log instrument settings called from: reducePressure
00:04:44.9	--------------------------------------------------------
00:04:44.9	--- HomeSpeed: 2000; PurgeSpeed: 2000
00:04:44.9	--- PressureTrackingExcessFactor: 96
00:04:44.9	--- FlowResistanceA: 300; FlowResistanceB: 100; FlowResistanceOut: 150
00:04:44.9	--- FC_FlowFilterDepth: 5; FC_PressureFilterDepth: 0
00:04:44.9	--- PC_OutputFilterDepth: 0
00:04:44.9	--- CompressibilityFactorB: 2.29999995231628; PressurePIDNegativeResultDivisor: 1
00:04:44.9	--- ExternalZeroPressureOffsetA: 130.944732666016; ExternalZeroPressureOffsetB: 128.061676025391
00:04:44.9	--- ExternalPressureScalingFactorA: 0.0197199992835522; ExternalPressureScalingFactorB: 0.0197199992835522
00:04:44.9	--------------------------------------------------------
00:04:44.9	--- Final pressure channel A: 216.856887817383
00:04:44.9	--- Final pressure channel B: 216.856887817383
00:04:44.9	-------------------------------------------------------
00:04:45.0	pump: SetMaxPressureLimit(A, 237.5)
00:04:45.0	pump: GetMaxPressureLimit(A) --> 237.5
00:04:45.2	autosampler-none: MoveValveDrive(Valve Drive 2, 60 deg)
00:04:45.2	-------------------------------------------------------
00:04:45.2	--- Trap Column Equilibration
00:04:45.2	-------------------------------------------------------
00:04:45.2	--- Trap Temperature: fixed to 20 C
00:04:45.2	--- Trap Unity-Flow  @ 20C [ul/bar]:      2.78838695282287
00:04:45.2	--- Trap Volume [ul]:                     0.148440252882118
00:04:45.2	--- Trap Equilibration Volume Multiplier: 10
00:04:45.2	--- Trap Equilibration Pressure [bar]:    217.5
00:04:45.2	--- Trap Equilibration Flow [ul/min]:     9.99557465783688
00:04:45.2	--- Trap Equilibration Volume (+5) [ul]:  6.48440252882118
00:04:45.2	--- Trap Equilibration Time [min]:        0.648727336925764
00:04:45.2	--- max. Volume (10*V+2ul/min*t) [ul]:    66.1414799620633
00:04:45.2	-------------------------------------------------------
00:04:45.2	pump: Manualmode_Pump_constantPressure(A, 217.5)
00:04:45.2	pump: GetPistonPosition(A) --> 473.356122047478
00:04:46.1	autosampler-left: PenetrateWithBottomSense(Rack 2, 12, 1 mm)
00:04:48.1	autosampler-left: AspirateSyringe(5 uL, 5 uL/s, 500 ms)
00:04:55.6	autosampler-left: LeaveObject()
00:04:56.4	autosampler-left: AspirateSyringe(1 uL, 5 uL/s, 500 ms)
00:04:59.8	autosampler-left: MoveToObject(LCMS Wash 1, 1, True, True, True)
00:05:00.8	autosampler-left: PenetrateObject(LCMS Wash 1, 1, 30 mm)
00:05:05.9	autosampler-left: Wait(5 s)
00:05:06.7	autosampler-left: LeaveObject()
00:05:09.9	autosampler-left: MoveToObject(LCInjector1)
00:05:10.8	autosampler-left: DispenseSyringe(0.75 uL, 1 uL/s)
00:05:11.2	pump: GetPistonPosition(A) --> 476.793024410505
00:05:11.2	pump: GetPistonPosition(A) --> 476.793024410505
00:05:11.2	pump: GetPistonPosition(A) --> 476.793024410505
00:05:13.4	autosampler-left: PenetrateWithConstForce(LCInjector1)
00:05:13.5	autosampler-left: MoveValveDrive(Valve Drive 1, 0 deg)
00:05:14.6	autosampler-left: Wait(1000 ms)
00:05:21.4	autosampler-left: DispenseSyringe(6.75 uL, 1 uL/s)
00:05:22.7	autosampler-left: Wait(1000 ms)
00:05:23.2	status: RemoveStatus(aspirate & inject)
00:05:50.3	pump: GetPistonPosition(A) --> 482.014141961261
00:05:50.3	pump: GetPistonPosition(A) --> 482.014141961261
00:06:00.3	pump: GetPistonPosition(A) --> 483.336333589402
00:06:00.3	pump: GetCurrentPressure(A) --> 217.3499
00:06:00.3	-------------------------------------------------------
00:06:00.3	--- Finished: Trap Column Equilibration (const_pressure_load_and_equilibration)
00:06:00.3	-------------------------------------------------------
00:06:00.3	--- act. pressure [bar]:           217.349899291992
00:06:00.3	--- used volume [uL]:              6.54330917889678
00:06:00.3	--- average flow [uL/min]:         8.0063331171622
00:06:00.3	--- act. equilibration time [min]: 0.817266666668002
00:06:00.3	-------------------------------------------------------
00:06:00.3	status: RemoveStatus(equilibrate trap-column, this finishes at about: 5.65 min.)
00:06:02.3	--- MaxPressureLimitA: 217.5 bar ---
00:06:02.3	pump: GetCurrentPressure(A) --> 217.4091
00:06:02.3	pump: GetCurrentPressure() --> 217.4091
00:06:02.3	pump: GetSettings() --> PumpSettings
00:06:02.3	pump: GetSetManualValvePosition(A) --> 0
00:06:02.3	-------------------------------------------------------
00:06:02.3	--- Reduce pressure ---
00:06:02.3	-------------------------------------------------------
00:06:02.3	--- Pressure channel A: 217.409057617188
00:06:02.3	--- Pressure channel B: 217.409057617188
00:06:02.3	pump: Manualmode_Pump_constantSpeed(A, 0)
00:06:02.3	pump: GetSetPistonSpeed(A) --> 0
00:06:02.3	pump: Manualmode_Pump_constantSpeed(, 0)
00:06:02.3	pump: GetSetPistonSpeed() --> 0
00:06:02.3	pump: IsIdle() --> False
00:06:02.3	-------------------------------------------------------
00:06:02.4	pump: SetMaxPressureLimit(A, 237.5)
00:06:02.4	pump: GetMaxPressureLimit(A) --> 237.5
00:06:02.4	status: SetStatus(load trap-column, this finishes at about: 8.31 min., )
00:06:02.5	autosampler-left: MoveValveDrive(Valve Drive 1, 60 deg)
00:06:03.6	-------------------------------------------------------
00:06:03.6	--- Sample Loading on Trap Column
00:06:03.6	-------------------------------------------------------
00:06:03.6	--- Trap Temperature: fixed to 20 C
00:06:03.6	--- Trap Unity-Flow [ul/bar]:          2.78838695282287
00:06:03.6	--- Trap Volume [ul]:                  0.148440252882118
00:06:03.6	--- Sample Volume [ul]:                5
00:06:03.6	--- Sample Volume Multiplier:          4
00:06:03.6	--- Trap Loading Pressure [bar]:       217.5
00:06:03.6	--- Trap Loading Flow [uL/min]:        9.99557465783688
00:06:03.6	--- Trap Loading Volume (+2) [uL]:     22
00:06:03.6	--- Trap Loading Time [min]:           2.200974006307
00:06:03.6	--- max. Volume (10*V+2ul/min*t) [ul]: 224.401948012614
00:06:03.6	-------------------------------------------------------
00:06:03.6	pump: Manualmode_Pump_constantPressure(A, 217.5)
00:06:03.6	pump: GetPistonPosition(A) --> 483.61425981949
00:06:03.7	autosampler-left: EmptySyringe(5 uL/s)
00:06:22.6	pump: GetPistonPosition(A) --> 486.764020613979
00:06:22.6	pump: GetPistonPosition(A) --> 486.764020613979
00:06:22.6	pump: GetPistonPosition(A) --> 486.764020613979
00:07:02.4	autosampler-left: CleanInjector(LCInjector1, LCP 1, 200 uL, 0 mm, 5 uL/s, 5 s, 20 mm, 40 uL/s, 1000 ms, 2, False)
00:08:03.5	autosampler-left: CleanInjector(LCInjector1, LCP 1, 200 uL, 0 mm, 5 uL/s, 5 s, 20 mm, 40 uL/s, 1000 ms, 1, True)
00:08:19.1	autosampler-left: CleanSyringe(LCMS Wash 1, 2, 30 mm, 40 uL/s, 500 ms, 30 mm, 40 uL/s, 80 %, 2)
00:08:34.7	pump: GetPistonPosition(A) --> 504.037544220967
00:08:34.7	pump: GetPistonPosition(A) --> 504.037544220967
00:08:39.7	autosampler-left: CleanSyringe(LCMS Wash 1, 1, 30 mm, 40 uL/s, 500 ms, 30 mm, 40 uL/s, 80 %, 3)
00:08:42.8	autosampler-left: MoveToHome()
00:09:10.8	pump: GetPistonPosition(A) --> 508.768573317763
00:09:10.8	pump: GetCurrentPressure(A) --> 217.4485
00:09:10.8	-------------------------------------------------------
00:09:10.8	--- Finished: Sample Loading on Trap Column (const_pressure_load_and_equilibration)
00:09:10.8	-------------------------------------------------------
00:09:10.8	--- act. pressure [bar]:     217.448501586914
00:09:10.8	--- used volume [uL]:        22.0045527037839
00:09:10.8	--- average flow [uL/min]:   7.85306600104234
00:09:10.8	--- act. loading time [min]: 2.8020333333329
00:09:10.8	-------------------------------------------------------
00:09:10.8	--- Separator: Name: IonOpticks 25 cm, Func: Separator, ColDia: 0.075 mm, Len: 250 mm, PartDia: 1.6 µm, Porosity: 42 % ---
00:09:10.8	--- Trap: Name: Thermo Trap Cartridge 5mm, Func: Separator, ColDia: 0.3 mm, Len: 5 mm, PartDia: 5 µm, Porosity: 42 % ---
00:09:10.8	--- MaxPressureLimitAB: 1000 bar ---
00:09:10.8	pump: GetCurrentPressure(A) --> 217.4485
00:09:10.8	pump: GetCurrentPressure(B) --> 0
00:09:10.8	pump: GetSettings() --> PumpSettings
00:09:10.8	pump: GetSetManualValvePosition(A) --> 0
00:09:10.8	-------------------------------------------------------
00:09:10.8	--- Reduce pressure ---
00:09:10.8	-------------------------------------------------------
00:09:10.8	--- Pressure channel A: 217.448501586914
00:09:10.8	--- Pressure channel B: 0
00:09:10.8	pump: Manualmode_Pump_constantSpeed(A, 0)
00:09:10.8	pump: GetSetPistonSpeed(A) --> 0
00:09:10.8	pump: Manualmode_Pump_constantSpeed(B, 0)
00:09:10.8	pump: GetSetPistonSpeed(B) --> 0
00:09:10.8	pump: IsIdle() --> False
00:09:10.8	-------------------------------------------------------
00:09:10.9	pump: SetMaxPressureLimit(A, 1020)
00:09:10.9	pump: GetMaxPressureLimit(A) --> 1020
00:09:11.0	pump: SetMaxPressureLimit(B, 1020)
00:09:11.0	pump: GetMaxPressureLimit(B) --> 1020
00:09:11.0	status: RemoveStatus(load trap-column, this finishes at about: 8.31 min.)
00:09:11.0	pump: GetSettings() --> PumpSettings
00:09:11.0	------------------------------------------------------------------
00:09:11.0	PID settings changed:
00:09:11.0	------------------------------------------------------------------
00:09:11.0	P: 100 : True; Pmin: -10000 : True; Pmax: 10000 : True
00:09:11.0	I: 100 : True; Imin: -1000 : True; Imax: 1000 : True; IWindow: 100 : True
00:09:11.0	D: 5000 : True; Dmin: -32000 : True; Dmax: 32000 : True
00:09:11.0	Result min: -5000 : True; Result max: 25000 : True
00:09:11.0	------------------------------------------------------------------
00:09:11.0	pump: SetSettings(PumpSettings)
00:09:11.0	pump: GetSettings() --> PumpSettings
00:09:11.0	------------------------------------------------------------------
00:09:11.0	PID settings changed:
00:09:11.0	------------------------------------------------------------------
00:09:11.0	P: 100 : False; Pmin: -10000 : False; Pmax: 10000 : False
00:09:11.0	I: 100 : False; Imin: -1000 : False; Imax: 1000 : False; IWindow: 2 : True
00:09:11.0	D: 5000 : False; Dmin: -32000 : False; Dmax: 32000 : False
00:09:11.0	Result min: -5000 : False; Result max: 25000 : False
00:09:11.0	------------------------------------------------------------------
00:09:11.0	pump: SetSettings(PumpSettings)
00:09:11.0	status: SetStatus(preparing gradient start, )
00:09:11.0	--- Starting conditioning paths ---
00:09:11.0	pump: GetSettings() --> PumpSettings
00:09:11.0	------------------------------------------------------------------
00:09:11.0	PID settings changed:
00:09:11.0	------------------------------------------------------------------
00:09:11.0	P: 100 : True; Pmin: -10000 : True; Pmax: 10000 : True
00:09:11.0	I: 100 : True; Imin: -1000 : True; Imax: 1000 : True; IWindow: 100 : True
00:09:11.0	D: 5000 : True; Dmin: -32000 : True; Dmax: 32000 : True
00:09:11.0	Result min: -5000 : True; Result max: 25000 : True
00:09:11.0	------------------------------------------------------------------
00:09:11.0	pump: SetSettings(PumpSettings)
00:09:11.1	pump: Manualmode_Pump_constantPressure(A, 0)
00:09:11.1	pump: GetTargetPressure(A) --> 0
00:09:11.1	pump: GetCurrentPressure(A) --> 217.3893
00:09:12.1	pump: GetCurrentPressure(A) --> 201.5345
00:09:13.1	pump: GetCurrentPressure(A) --> 179.6255
00:09:14.1	pump: GetCurrentPressure(A) --> 158.0913
00:09:15.1	pump: GetCurrentPressure(A) --> 135.8471
00:09:16.1	pump: GetCurrentPressure(A) --> 114.3129
00:09:17.1	pump: GetCurrentPressure(A) --> 92.60118
00:09:18.1	pump: GetCurrentPressure(A) --> 71.67826
00:09:19.1	pump: GetCurrentPressure(A) --> 52.94426
00:09:20.1	pump: GetCurrentPressure(A) --> 32.88902
00:09:21.1	pump: GetCurrentPressure(A) --> 19.34138
00:09:22.1	pump: GetCurrentPressure(A) --> 12.41966
00:09:22.3	autosampler-none: MoveValveDrive(Valve Drive 2, 0 deg)
00:09:22.3	pump: SetManualValvePosition(A, 240, False)
00:09:22.9	pump: SetManualValvePosition(B, 240, False)
00:09:24.9	--- Set constant binary flow ---
00:09:24.9	pump: Manualmode_Pump_constantFlow_binary(1, 1)
00:09:24.9	pump: GetSetFlow(A) --> 1
00:09:24.9	pump: GetSetFlow(B) --> 1
00:09:24.9	

00:09:24.9	--- Starting spline flow reduction B/A ---
00:09:24.9	pump: GetSetExternalTemperature() --> 50
00:09:24.9	--- Oven temperatur: 50
00:09:24.9	--- Separator: Name: IonOpticks 25 cm, Func: Separator, ColDia: 0.075 mm, Len: 250 mm, PartDia: 1.6 µm, Porosity: 42 %
00:09:24.9	--- Spline fitting time: 97.6577595902957 s
00:09:24.9	--- Spline fitting time adapted: 10 s
00:09:24.9	--- y = 0x + 1
00:09:24.9	--- p0.Time -1, p0.Flow 1
00:09:24.9	--- p1.Time 0, p1.Flow 1
00:09:24.9	--- p2.Time 9, p2.Flow 1
00:09:24.9	--- p3.Time 10, p3.Flow 1, p3.Mix 0
00:09:24.9	--- p4.Time 40, p4.Flow 1, p4.Mix 0
00:09:24.9	--- Strategy function end
00:09:24.9	--- y = 0x + 0
00:09:24.9	--- p0.Time -1, p0.Flow 1
00:09:24.9	--- p1.Time 0, p1.Flow 1
00:09:24.9	--- p2.Time 9, p2.Flow 0
00:09:24.9	--- p3.Time 10, p3.Flow 0, p3.Mix 0
00:09:24.9	--- p4.Time 40, p4.Flow 0, p4.Mix 0
00:09:24.9	--- Strategy function end
00:09:24.9	xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx
00:09:24.9	XXXXX Time 0, Flow 2, Mix 50
00:09:24.9	XXXXX Time 1, Flow 1.94545, Mix 48.59813
00:09:24.9	XXXXX Time 2, Flow 1.84848, Mix 45.90163
00:09:25.0	XXXXX Time 3, Flow 1.72121, Mix 41.9014
00:09:25.0	XXXXX Time 4, Flow 1.57575, Mix 36.53846
00:09:25.0	XXXXX Time 5, Flow 1.42424, Mix 29.78723
00:09:25.0	XXXXX Time 6, Flow 1.27878, Mix 21.80094
00:09:25.0	XXXXX Time 7, Flow 1.15151, Mix 13.15789
00:09:25.0	XXXXX Time 8, Flow 1.05454, Mix 5.17241
00:09:25.0	XXXXX Time 9, Flow 1, Mix 0
00:09:25.0	--- Gradient is created, switching valves A and B
00:09:25.0	pump: GetSetManualValvePosition(A) --> 240
00:09:25.0	pump: GetCurrentPressure(A) --> 4.216143
00:09:25.0	pump: SetManualValvePosition(A, 240, False)
00:09:25.2	pump: GetSetManualValvePosition(B) --> 240
00:09:25.2	pump: GetCurrentPressure(B) --> 0
00:09:25.2	pump: SetManualValvePosition(B, 240, False)
00:09:25.4	--- Valves A and B switched to MixTee, starting gradient
00:09:25.4	--- LoadGradient() ---
00:09:25.4	--- Loading gradient method index: 0
00:09:25.4	pump: LoadMethod(0, ZrData.ZrPumpMethod)
00:09:25.9	pump: GetMethodState(0) --> loaded
00:09:25.9	--- Set gradient start
00:09:25.9	--- StartGradient() ---
00:09:25.9	--- Starting gradient method index: 0
00:09:25.9	pump: SetMethodSignal(0, StartTrunk)
00:09:26.1	pump: GetMethodState(0) --> runningTrunk
00:09:26.1	--- Start GradientSplineFit
00:09:26.1	pump: GetMethodState(0) --> runningTrunk
00:09:27.1	pump: GetCurrentPressure(A) --> 78.6197
00:09:27.1	pump: GetCurrentPressure(B) --> 46.2434
00:09:27.1	--- GradientSplineFit is running
00:09:35.1	--- GradientSplineFit has finished
00:09:35.1	--- End GradientSplineFit
00:09:35.1	--- Finished spline flow reduction B ---
00:09:36.2	pump: GetSetExternalTemperature() --> 50
00:09:36.2	--- Oven temperatur: 50
00:09:36.2	--- Separator: Name: IonOpticks 25 cm, Func: Separator, ColDia: 0.075 mm, Len: 250 mm, PartDia: 1.6 µm, Porosity: 42 %
00:09:36.2	--- Spline fitting time: 97.6577595902957 s
00:09:36.2	--- Spline fitting time adapted: 60 s
00:09:36.2	--- y = 0x + 0.01
00:09:36.2	--- p0.Time -1, p0.Flow 1
00:09:36.2	--- p1.Time 0, p1.Flow 1
00:09:36.2	--- p2.Time 59, p2.Flow 0.01
00:09:36.2	--- p3.Time 60, p3.Flow 0.01, p3.Mix 0
00:09:36.2	--- p4.Time 90, p4.Flow 0.01, p4.Mix 0
00:09:36.2	--- Strategy function end
00:09:36.2	--- y = 0x + 0
00:09:36.2	--- p0.Time -1, p0.Flow 0
00:09:36.2	--- p1.Time 0, p1.Flow 0
00:09:36.2	--- p2.Time 59, p2.Flow 0
00:09:36.2	--- p3.Time 60, p3.Flow 0, p3.Mix 0
00:09:36.2	--- p4.Time 90, p4.Flow 0, p4.Mix 0
00:09:36.2	--- Strategy function end
00:09:36.2	xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx
00:09:36.2	XXXXX Time 0, Flow 1, Mix 0
00:09:36.2	XXXXX Time 1, Flow 0.99837, Mix 0
00:09:36.2	XXXXX Time 2, Flow 0.99518, Mix 0
00:09:36.2	XXXXX Time 3, Flow 0.99048, Mix 0
00:09:36.2	XXXXX Time 4, Flow 0.98432, Mix 0
00:09:36.2	XXXXX Time 5, Flow 0.97675, Mix 0
00:09:36.2	XXXXX Time 6, Flow 0.96784, Mix 0
00:09:36.2	XXXXX Time 7, Flow 0.95763, Mix 0
00:09:36.2	XXXXX Time 8, Flow 0.94619, Mix 0
00:09:36.2	XXXXX Time 9, Flow 0.93356, Mix 0
00:09:36.2	XXXXX Time 10, Flow 0.91981, Mix 0
00:09:36.2	XXXXX Time 11, Flow 0.90498, Mix 0
00:09:36.2	XXXXX Time 12, Flow 0.88914, Mix 0
00:09:36.2	XXXXX Time 13, Flow 0.87233, Mix 0
00:09:36.3	XXXXX Time 14, Flow 0.85462, Mix 0
00:09:36.3	XXXXX Time 15, Flow 0.83605, Mix 0
00:09:36.3	XXXXX Time 16, Flow 0.81668, Mix 0
00:09:36.3	XXXXX Time 17, Flow 0.79658, Mix 0
00:09:36.3	XXXXX Time 18, Flow 0.77578, Mix 0
00:09:36.3	XXXXX Time 19, Flow 0.75435, Mix 0
00:09:36.3	XXXXX Time 20, Flow 0.73235, Mix 0
00:09:36.3	XXXXX Time 21, Flow 0.70982, Mix 0
00:09:36.3	XXXXX Time 22, Flow 0.68682, Mix 0
00:09:36.3	XXXXX Time 23, Flow 0.66341, Mix 0
00:09:36.3	XXXXX Time 24, Flow 0.63964, Mix 0
00:09:36.3	XXXXX Time 25, Flow 0.61558, Mix 0
00:09:36.3	XXXXX Time 26, Flow 0.59126, Mix 0
00:09:36.3	XXXXX Time 27, Flow 0.56675, Mix 0
00:09:36.3	XXXXX Time 28, Flow 0.5421, Mix 0
00:09:36.3	XXXXX Time 29, Flow 0.51737, Mix 0
00:09:36.3	XXXXX Time 30, Flow 0.49262, Mix 0
00:09:36.4	XXXXX Time 31, Flow 0.46789, Mix 0
00:09:36.4	XXXXX Time 32, Flow 0.44324, Mix 0
00:09:36.4	XXXXX Time 33, Flow 0.41873, Mix 0
00:09:36.4	XXXXX Time 34, Flow 0.39441, Mix 0
00:09:36.4	XXXXX Time 35, Flow 0.37035, Mix 0
00:09:36.4	XXXXX Time 36, Flow 0.34658, Mix 0
00:09:36.4	XXXXX Time 37, Flow 0.32317, Mix 0
00:09:36.4	XXXXX Time 38, Flow 0.30017, Mix 0
00:09:36.4	XXXXX Time 39, Flow 0.27764, Mix 0
00:09:36.4	XXXXX Time 40, Flow 0.25564, Mix 0
00:09:36.4	XXXXX Time 41, Flow 0.23421, Mix 0
00:09:36.4	XXXXX Time 42, Flow 0.21341, Mix 0
00:09:36.4	XXXXX Time 43, Flow 0.19331, Mix 0
00:09:36.4	XXXXX Time 44, Flow 0.17394, Mix 0
00:09:36.4	XXXXX Time 45, Flow 0.15537, Mix 0
00:09:36.4	XXXXX Time 46, Flow 0.13766, Mix 0
00:09:36.5	XXXXX Time 47, Flow 0.12085, Mix 0
00:09:36.5	XXXXX Time 48, Flow 0.10501, Mix 0
00:09:36.5	XXXXX Time 49, Flow 0.09018, Mix 0
00:09:36.5	XXXXX Time 50, Flow 0.07643, Mix 0
00:09:36.5	XXXXX Time 51, Flow 0.0638, Mix 0
00:09:36.5	XXXXX Time 52, Flow 0.05236, Mix 0
00:09:36.5	XXXXX Time 53, Flow 0.04215, Mix 0
00:09:36.5	XXXXX Time 54, Flow 0.03324, Mix 0
00:09:36.5	XXXXX Time 55, Flow 0.02567, Mix 0
00:09:36.5	XXXXX Time 56, Flow 0.01951, Mix 0
00:09:36.5	XXXXX Time 57, Flow 0.01481, Mix 0
00:09:36.5	XXXXX Time 58, Flow 0.01162, Mix 0
00:09:36.5	XXXXX Time 59, Flow 0.00999, Mix 0
00:09:36.5	--- Gradient is created, switching valves A and B
00:09:36.5	pump: GetSetManualValvePosition(A) --> 240
00:09:36.5	pump: GetCurrentPressure(A) --> 89.2685
00:09:36.5	pump: SetManualValvePosition(A, 240, False)
00:09:36.7	pump: GetSetManualValvePosition(B) --> 240
00:09:36.7	pump: GetCurrentPressure(B) --> 12.30528
00:09:36.7	pump: SetManualValvePosition(B, 240, False)
00:09:36.9	--- Valves A and B switched to MixTee, starting gradient
00:09:36.9	--- LoadGradient() ---
00:09:36.9	--- Loading gradient method index: 0
00:09:36.9	pump: LoadMethod(0, ZrData.ZrPumpMethod)
00:09:37.4	pump: GetMethodState(0) --> loaded
00:09:37.4	--- Set gradient start
00:09:37.4	--- StartGradient() ---
00:09:37.4	--- Starting gradient method index: 0
00:09:37.4	pump: SetMethodSignal(0, StartTrunk)
00:09:37.6	pump: GetMethodState(0) --> runningTrunk
00:09:37.6	--- Start GradientSplineFit
00:09:37.6	pump: GetMethodState(0) --> runningTrunk
00:09:38.6	pump: GetCurrentPressure(A) --> 90.37282
00:09:38.6	pump: GetCurrentPressure(B) --> 13.68568
00:09:38.6	--- GradientSplineFit is running
00:10:36.7	--- GradientSplineFit has finished
00:10:36.7	--- End GradientSplineFit
00:10:36.7	--- Finished spline flow reduction A ---

00:10:36.7	--- Finished conditioning paths ---
00:10:42.2	autosampler-none: MoveValveDrive(Valve Drive 2, 240 deg)
00:10:42.3	pump: GetSetExternalTemperature() --> 50
00:10:42.3	--- Oven temperatur: 50
00:10:42.3	--- Separator: Name: IonOpticks 25 cm, Func: Separator, ColDia: 0.075 mm, Len: 250 mm, PartDia: 1.6 µm, Porosity: 42 %
00:10:42.3	--- Spline fitting time: 97.6577595902957 s
00:10:42.3	--- Spline fitting time adapted: 53.7117677746626 s
00:10:42.3	--- Press: 541.893115083548; colPress: 511.893115083548, visco: 0.00566684100509561, flow > 0.3
00:10:42.3	--- Flow: 0.3; Mix: 0, calcI: 220.674819441516
00:10:42.3	--- Ki calc: 220.674819441516
00:10:42.3	pump: GetSettings() --> PumpSettings
00:10:42.3	------------------------------------------------------------------
00:10:42.3	PID settings changed:
00:10:42.3	------------------------------------------------------------------
00:10:42.3	P: 200 : True; Pmin: -32000 : True; Pmax: 32000 : True
00:10:42.3	I: 600 : True; Imin: -32000 : True; Imax: 32000 : True; IWindow: 100 : True
00:10:42.3	D: 1000 : True; Dmin: -32000 : True; Dmax: 32000 : True
00:10:42.3	Result min: -32000 : True; Result max: 32000 : True
00:10:42.3	------------------------------------------------------------------
00:10:42.3	pump: SetSettings(PumpSettings)
00:10:42.3	pump: GetSettings() --> PumpSettings
00:10:42.3	------------------------------------------------------------------
00:10:42.3	PID settings changed:
00:10:42.3	------------------------------------------------------------------
00:10:42.3	P: 200 : False; Pmin: -32000 : False; Pmax: 32000 : False
00:10:42.3	I: 220 : True; Imin: -32000 : False; Imax: 32000 : False; IWindow: 100 : False
00:10:42.3	D: 1000 : False; Dmin: -32000 : False; Dmax: 32000 : False
00:10:42.3	Result min: -32000 : False; Result max: 32000 : False
00:10:42.3	------------------------------------------------------------------
00:10:42.3	pump: SetSettings(PumpSettings)
00:10:42.3	--- y = -5.33333333333333E-05x + 0.302864627614649
00:10:42.3	--- p0.Time -1, p0.Flow 0.01
00:10:42.3	--- p1.Time 0, p1.Flow 0.01
00:10:42.3	--- p2.Time 52.7117677746626, p2.Flow 0.300053333333333
00:10:42.3	--- p3.Time 53.7117677746626, p3.Flow 0.3, p3.Mix 0
00:10:42.3	--- p4.Time 1853.71176777466, p4.Flow 0.204, p4.Mix 32
00:10:42.3	--- Strategy function end
00:10:42.3	--- y = 5.33333333333333E-05x + -0.00286462761464867
00:10:42.3	--- p0.Time -1, p0.Flow 0
00:10:42.3	--- p1.Time 0, p1.Flow 0
00:10:42.3	--- p2.Time 52.7117677746626, p2.Flow -5.33333333333336E-05
00:10:42.3	--- p3.Time 53.7117677746626, p3.Flow 0, p3.Mix 0
00:10:42.3	--- p4.Time 1853.71176777466, p4.Flow 0.096, p4.Mix 32
00:10:42.3	--- Strategy function end
00:10:42.3	xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx
00:10:42.3	XXXXX Time 0, Flow 0.01, Mix 0
00:10:42.3	XXXXX Time 1, Flow 0.01059, Mix 0
00:10:42.4	XXXXX Time 2, Flow 0.01175, Mix 0
00:10:42.4	XXXXX Time 3, Flow 0.01347, Mix 0
00:10:42.4	XXXXX Time 4, Flow 0.01571, Mix 0
00:10:42.4	XXXXX Time 5, Flow 0.01845, Mix 0
00:10:42.4	XXXXX Time 6, Flow 0.02168, Mix 0
00:10:42.4	XXXXX Time 7, Flow 0.02537, Mix 0
00:10:42.4	XXXXX Time 8, Flow 0.02949, Mix 0
00:10:42.4	XXXXX Time 9, Flow 0.03402, Mix 0
00:10:42.4	XXXXX Time 10, Flow 0.03894, Mix 0
00:10:42.4	XXXXX Time 11, Flow 0.04424, Mix 0
00:10:42.4	XXXXX Time 12, Flow 0.04988, Mix 0
00:10:42.4	XXXXX Time 13, Flow 0.05584, Mix 0
00:10:42.4	XXXXX Time 14, Flow 0.0621, Mix 0
00:10:42.4	XXXXX Time 15, Flow 0.06864, Mix 0
00:10:42.5	XXXXX Time 16, Flow 0.07544, Mix 0
00:10:42.5	XXXXX Time 17, Flow 0.08247, Mix 0
00:10:42.5	XXXXX Time 18, Flow 0.08971, Mix 0
00:10:42.5	XXXXX Time 19, Flow 0.09713, Mix 0
00:10:42.5	XXXXX Time 20, Flow 0.10472, Mix 0
00:10:42.5	XXXXX Time 21, Flow 0.11246, Mix 0
00:10:42.5	XXXXX Time 22, Flow 0.12031, Mix 0
00:10:42.5	XXXXX Time 23, Flow 0.12826, Mix 0
00:10:42.5	XXXXX Time 24, Flow 0.13629, Mix 0
00:10:42.5	XXXXX Time 25, Flow 0.14437, Mix 0
00:10:42.5	XXXXX Time 26, Flow 0.15248, Mix 0
00:10:42.5	XXXXX Time 27, Flow 0.16059, Mix 0
00:10:42.5	XXXXX Time 28, Flow 0.16869, Mix 0
00:10:42.5	XXXXX Time 29, Flow 0.17675, Mix 0
00:10:42.5	XXXXX Time 30, Flow 0.18476, Mix 0
00:10:42.5	XXXXX Time 31, Flow 0.19267, Mix 0
00:10:42.6	XXXXX Time 32, Flow 0.20049, Mix 0
00:10:42.6	XXXXX Time 33, Flow 0.20817, Mix 0
00:10:42.6	XXXXX Time 34, Flow 0.2157, Mix 0
00:10:42.6	XXXXX Time 35, Flow 0.22306, Mix 0
00:10:42.6	XXXXX Time 36, Flow 0.23022, Mix 0
00:10:42.6	XXXXX Time 37, Flow 0.23717, Mix 0
00:10:42.6	XXXXX Time 38, Flow 0.24387, Mix 0
00:10:42.6	XXXXX Time 39, Flow 0.25031, Mix 0
00:10:42.6	XXXXX Time 40, Flow 0.25646, Mix 0
00:10:42.6	XXXXX Time 41, Flow 0.2623, Mix 0
00:10:42.6	XXXXX Time 42, Flow 0.26781, Mix 0
00:10:42.6	XXXXX Time 43, Flow 0.27297, Mix 0
00:10:42.6	XXXXX Time 44, Flow 0.27775, Mix 0
00:10:42.6	XXXXX Time 45, Flow 0.28213, Mix 0
00:10:42.6	XXXXX Time 46, Flow 0.28609, Mix 0
00:10:42.6	XXXXX Time 47, Flow 0.28961, Mix 0
00:10:42.7	XXXXX Time 48, Flow 0.29265, Mix 0
00:10:42.7	XXXXX Time 49, Flow 0.29521, Mix 0
00:10:42.7	XXXXX Time 50, Flow 0.29726, Mix 0
00:10:42.7	XXXXX Time 51, Flow 0.29877, Mix 0
00:10:42.7	XXXXX Time 52, Flow 0.29972, Mix 0
00:10:42.7	XXXXX Time 53, Flow 0.3001, Mix 0
00:10:42.7	--- Gradient is created, switching valves A and B
00:10:42.7	pump: GetSetManualValvePosition(A) --> 240
00:10:42.7	pump: GetCurrentPressure(A) --> 0.7454225
00:10:42.7	pump: SetManualValvePosition(A, 240, False)
00:10:42.9	pump: GetSetManualValvePosition(B) --> 240
00:10:42.9	pump: GetCurrentPressure(B) --> 0
00:10:42.9	pump: SetManualValvePosition(B, 240, False)
00:10:43.1	--- Valves A and B switched to MixTee, starting gradient
00:10:43.1	--- LoadGradient() ---
00:10:43.1	--- Loading gradient method index: 0
00:10:43.1	pump: LoadMethod(0, ZrData.ZrPumpMethod)
00:10:43.6	pump: GetMethodState(0) --> loaded
00:10:43.6	--- Set gradient start
00:10:43.6	--- StartGradient() ---
00:10:43.6	--- Starting gradient method index: 0
00:10:43.6	pump: SetMethodSignal(0, StartTrunk)
00:10:43.8	pump: GetMethodState(0) --> runningTrunk
00:10:43.8	--- Start GradientSplineFit
00:10:43.8	pump: GetMethodState(0) --> runningTrunk
00:10:44.8	pump: GetCurrentPressure(A) --> 0
00:10:44.8	pump: GetCurrentPressure(B) --> 0
00:10:44.8	--- GradientSplineFit is running
00:11:36.8	--- GradientSplineFit has finished
00:11:36.8	--- End GradientSplineFit
00:11:36.8	status: RemoveStatus(preparing gradient start)
00:11:36.9	Setting signal "lc-ready"
00:11:36.9	Waiting for signal "ms-start"...
00:11:36.9	--- LoadGradient() ---
00:11:36.9	--- Loading gradient method index: 0
00:11:36.9	pump: LoadMethod(0, ZrData.ZrPumpMethod)
00:11:37.4	pump: GetMethodState(0) --> loaded
00:11:37.4	--- Set gradient start
00:11:37.4	--- StartGradient() ---
00:11:37.4	--- Starting gradient method index: 0
00:11:37.4	pump: SetMethodSignal(0, StartTrunk)
00:11:37.6	pump: GetMethodState(0) --> runningTrunk
00:11:37.7	autosampler-none: SetSignal(Output Signal 1)
00:11:37.7	status: SetStatus(wash, )
00:11:41.3	status: SetStatus(elution, )
00:11:43.0	autosampler-left: MoveToObject(LCInjector1)
00:11:45.6	autosampler-left: PenetrateWithConstForce(LCInjector1)
00:11:45.8	autosampler-left: MoveValveDrive(Valve Drive 1, 0 deg)
00:12:15.6	autosampler-left: CleanInjector(LCInjector1, LCP 1, 100 uL, 0 mm, 5 uL/s, 5 s, 20 mm, 40 uL/s, 1000 ms, 2, False)
00:13:45.6	autosampler-left: CleanInjector(LCInjector1, LCP 1, 300 uL, 0 mm, 5 uL/s, 5 s, 20 mm, 40 uL/s, 1000 ms, 1, True)
00:13:45.8	autosampler-left: MoveValveDrive(Valve Drive 1, 60 deg)
00:13:49.0	autosampler-left: MoveToHome()
01:11:38.3	Setting signal "lc-done"
01:11:38.3	status: RemoveStatus(elution)
01:11:38.3	status: RemoveStatus(wash)
01:11:38.3	pump: GetCurrentPressure(A) --> 243.4395
01:11:38.3	pump: GetCurrentPressure(B) --> 251.3117
01:11:38.3	pump: GetSettings() --> PumpSettings
01:11:38.3	pump: GetSetManualValvePosition(A) --> 240
01:11:38.3	pump: GetSetManualValvePosition(B) --> 240
01:11:38.3	-------------------------------------------------------
01:11:38.3	--- Reduce pressure ---
01:11:38.3	-------------------------------------------------------
01:11:38.3	--- Pressure channel A: 243.439453125
01:11:38.3	--- Pressure channel B: 251.311676025391
01:11:38.3	pump: GetSettings() --> PumpSettings
01:11:38.3	------------------------------------------------------------------
01:11:38.3	PID settings changed:
01:11:38.3	------------------------------------------------------------------
01:11:38.3	P: 250 : True; Pmin: -5500 : True; Pmax: 32000 : True
01:11:38.3	I: 50 : True; Imin: -20000 : True; Imax: 20000 : True; IWindow: 50 : True
01:11:38.3	D: 1500 : True; Dmin: -10000 : True; Dmax: 10000 : True
01:11:38.3	Result min: -32000 : True; Result max: 32000 : True
01:11:38.3	------------------------------------------------------------------
01:11:38.3	pump: SetSettings(PumpSettings)
01:11:38.5	--- Set pressure channel A:          10
01:11:38.5	--- Set pressure channel B:          5
01:11:38.5	--- Reduce pressure flow controlled: True
01:12:08.8	--------------------------------------------------------
01:12:08.8	--- Log PID parameter called from: reducePressure
01:12:08.8	--------------------------------------------------------
01:12:08.8	--- P: 200; Pmin: -32000; Pmax: 32000
01:12:08.8	--- I: 220; Imin: -32000; Imax: 32000; IWindow: 100
01:12:08.8	--- D: 1000; Dmin: -32000; Dmax: 32000
01:12:08.8	--- Result min: -32000; Result max: 32000
01:12:08.8	--------------------------------------------------------
01:12:08.8	--------------------------------------------------------
01:12:08.8	--- Log instrument settings called from: reducePressure
01:12:08.8	--------------------------------------------------------
01:12:08.8	--- HomeSpeed: 2000; PurgeSpeed: 2000
01:12:08.8	--- PressureTrackingExcessFactor: 96
01:12:08.8	--- FlowResistanceA: 300; FlowResistanceB: 100; FlowResistanceOut: 150
01:12:08.8	--- FC_FlowFilterDepth: 5; FC_PressureFilterDepth: 0
01:12:08.8	--- PC_OutputFilterDepth: 0
01:12:08.8	--- CompressibilityFactorB: 2.29999995231628; PressurePIDNegativeResultDivisor: 1
01:12:08.8	--- ExternalZeroPressureOffsetA: 130.944732666016; ExternalZeroPressureOffsetB: 128.061676025391
01:12:08.8	--- ExternalPressureScalingFactorA: 0.0197199992835522; ExternalPressureScalingFactorB: 0.0197199992835522
01:12:08.8	--------------------------------------------------------
01:12:08.8	--- Final pressure channel A: 6.05010223388672
01:12:08.8	--- Final pressure channel B: 4.37783908843994
01:12:08.8	-------------------------------------------------------
01:12:09.1	autosampler-left: MoveValveDrive(Valve Drive 2, 0 deg)
01:12:09.3	pump: Manualmode_Pump_constantSpeed(A, 0)
01:12:09.3	pump: GetSetPistonSpeed(A) --> 0
01:12:09.3	pump: Manualmode_Pump_constantSpeed(B, 0)
01:12:09.3	pump: GetSetPistonSpeed(B) --> 0
01:12:09.3	pump: IsIdle() --> True
01:12:09.8	autosampler-left: MoveValveDrive(Valve Drive 1, 60 deg)
01:12:11.7	autosampler-left: MoveToHome()
01:12:11.8	pump: IsIdle() --> True
01:12:11.8	COMPLETED