Path: 2022-07-30_14-39-59_Two-column-separation
Procedure: Two column separation
Argument list:
uses_trap = True
uses_separator = True
is_isocratic = False
analysis_time = 1800
oven_temperature = 50
sample_volume = 5
sample_position = Slot2:12
presample_solvent_volume = 2
presample_air_volume = 0.5
postsample_air_volume = 1
postsample_solvent_volume = 2
sample_aspirate_speed = 5
sample_postaspirate_delay = 500
sample_inject_speed = 1
calibrant_volume = 0.6
calibrantTime = 10
is_calibrant_inject = False
is_extended_wash = False
is_bottom_sense = True
injection_method = uLPickup
trap = Name: Thermo Trap Cartridge 5mm, Func: Separator, ColDia: 0.3 mm, Len: 5 mm, PartDia: 5 µm, Porosity: 42 %
trap_volume = 0.148440252882118
trap_unityflow = 2.78838695282287
trap_equilibration_volumemultiplier = 10
trap_equilibration_pressure = 217.5
trap_equil_time = 0.648440252882118
separator = Name: IonOpticks 25 cm, Func: Separator, ColDia: 0.075 mm, Len: 250 mm, PartDia: 1.6 µm, Porosity: 42 %
separator_volume = 0.463875790256618
separator_unityflow = 0.00058605984562038
separator_equilibration_volumemultiplier = 4
separator_equilibration_pressure = 800
column_load_volumemultiplier = 4
column_load_pressure = 217.5
separator_equil_time = 4.05402026133927
column_load_time = 0.6
gradient = (0 sec,0.3 µL/min,0.00 %), (1800 sec,0.3 µL/min,32.00 %), (2700 sec,0.3 µL/min,40.00 %), (3000 sec,0.3 µL/min,60.00 %), (3300 sec,0.3 µL/min,95.00 %), (3600 sec,0.3 µL/min,95.00 %)
00:00:00.0	STARTED
00:00:00.7	Lua version:		1.1.0
00:00:00.7	Lua date:			2020/01/30
00:00:00.7	--- Experiment:    Description
00:00:00.7	-------------------------------------------------------
00:00:00.7	--- Trap Column:              Thermo Trap Cartridge 5mm
00:00:00.7	---    Manufacturer:          Thermo Scientific
00:00:00.7	---    Part Number:           
00:00:00.7	---    Serial Number:         
00:00:00.7	---    Type/Material:         µ-Precolum TM Cartridge  / PepMap TM C18
00:00:00.7	---    Length:                5 mm
00:00:00.7	---    Inner Diameter:        0.3 mm
00:00:00.7	---    Particle Diameter:     5 um
00:00:00.7	---    Is Particle Pore Size: True
00:00:00.7	---    Particle Pore Size:    100
00:00:00.7	---    Is Column Porosity:    False
00:00:00.7	---    Column Porosity:       0.42 (0.0-1.0)
00:00:00.7	---    Is Max Temperature:    False
00:00:00.7	---    max Temperature:       65 C
00:00:00.7	---    Is Max Pressure:       False
00:00:00.7	---    max Pressure:          1000 bar
00:00:00.7	---    Is Max Flow:           False
00:00:00.7	---    max Flow:              9 uL/min
00:00:00.7	---    Advanced Settings:     False
00:00:00.7	---    Column Comments:       Manufacturer no: 160454
00:00:00.7	-------------------------------------------------------
00:00:00.7	--- Separation Column:        IonOpticks 25 cm
00:00:00.7	---    Manufacturer:          IonOpticks
00:00:00.7	---    Part Number:           
00:00:00.7	---    Serial Number:         
00:00:00.7	---    Type/Material:         C18
00:00:00.7	---    Length:                250 mm
00:00:00.7	---    Inner Diameter:        0.075 mm
00:00:00.7	---    Particle Diameter:     1.6 um
00:00:00.7	---    Is Particle Pore Size: True
00:00:00.7	---    Particle Pore Size:    120
00:00:00.7	---    Is Column Porosity:    False
00:00:00.7	---    Column Porosity:       0.42 (0.0-1.0)
00:00:00.7	---    Is Max Temperature:    False
00:00:00.7	---    max Temperature:       65 C
00:00:00.7	---    Is Max Pressure:       True
00:00:00.7	---    max Pressure:          1000 bar
00:00:00.7	---    Is Max Flow:           False
00:00:00.7	---    max Flow:              9 uL/min
00:00:00.7	---    Advanced Settings:     False
00:00:00.7	---    Column Comments:           
00:00:00.7	-------------------------------------------------------
00:00:00.7	'Valve Drive 1' is 'ILcInjectorValve'
00:00:00.7	'Valve Drive 2' is 'ISelectorValve'
00:00:00.7	--- Trap equil time:		0.64
00:00:00.7	--- Seperator equil time:	4.05
00:00:00.7	--- Column loading time:	0.6
00:00:00.7	pump: GetSettings() --> PumpSettings
00:00:00.7	--------------------------------------------------------
00:00:00.7	--- Log instrument settings called from: Two Column Separation
00:00:00.7	--------------------------------------------------------
00:00:00.7	--- HomeSpeed: 2000; PurgeSpeed: 2000
00:00:00.7	--- PressureTrackingExcessFactor: 96
00:00:00.7	--- FlowResistanceA: 300; FlowResistanceB: 100; FlowResistanceOut: 150
00:00:00.7	--- FC_FlowFilterDepth: 5; FC_PressureFilterDepth: 0
00:00:00.7	--- PC_OutputFilterDepth: 0
00:00:00.7	--- CompressibilityFactorB: 2.29999995231628; PressurePIDNegativeResultDivisor: 1
00:00:00.7	--- ExternalZeroPressureOffsetA: 130.944732666016; ExternalZeroPressureOffsetB: 128.061676025391
00:00:00.7	--- ExternalPressureScalingFactorA: 0.0197199992835522; ExternalPressureScalingFactorB: 0.0197199992835522
00:00:00.7	--------------------------------------------------------
00:00:00.7	--- Oven temperature: 50
00:00:00.7	--- Separator: Name: IonOpticks 25 cm, Func: Separator, ColDia: 0.075 mm, Len: 250 mm, PartDia: 1.6 µm, Porosity: 42 % ---
00:00:00.7	--- Trap: Name: Thermo Trap Cartridge 5mm, Func: Separator, ColDia: 0.3 mm, Len: 5 mm, PartDia: 5 µm, Porosity: 42 % ---
00:00:00.7	--- MaxPressureLimitAB: 1000 bar ---
00:00:00.7	pump: GetCurrentPressure(A) --> 6.365623
00:00:00.7	pump: GetCurrentPressure(B) --> 6.152639
00:00:00.7	pump: GetSettings() --> PumpSettings
00:00:00.7	pump: GetSetManualValvePosition(A) --> 240
00:00:00.7	pump: GetSetManualValvePosition(B) --> 240
00:00:00.7	-------------------------------------------------------
00:00:00.7	--- Reduce pressure ---
00:00:00.7	-------------------------------------------------------
00:00:00.7	--- Pressure channel A: 6.36562252044678
00:00:00.7	--- Pressure channel B: 6.15263891220093
00:00:00.7	pump: Manualmode_Pump_constantSpeed(A, 0)
00:00:00.7	pump: GetSetPistonSpeed(A) --> 0
00:00:00.7	pump: Manualmode_Pump_constantSpeed(B, 0)
00:00:00.7	pump: GetSetPistonSpeed(B) --> 0
00:00:00.7	pump: IsIdle() --> True
00:00:00.7	-------------------------------------------------------
00:00:00.8	pump: SetMaxPressureLimit(A, 1020)
00:00:00.8	pump: GetMaxPressureLimit(A) --> 1020
00:00:00.9	pump: SetMaxPressureLimit(B, 1020)
00:00:00.9	pump: GetMaxPressureLimit(B) --> 1020
00:00:00.9	pump: GetSettings() --> PumpSettings
00:00:00.9	------------------------------------------------------------------
00:00:00.9	PID settings changed:
00:00:00.9	------------------------------------------------------------------
00:00:00.9	P: 175 : True; Pmin: -5000 : True; Pmax: 5000 : True
00:00:00.9	I: 5 : True; Imin: -200 : True; Imax: 1000 : True; IWindow: 10 : True
00:00:00.9	D: 0 : True; Dmin: -1000 : True; Dmax: 1000 : True
00:00:00.9	Result min: -6000 : True; Result max: 8000 : True
00:00:00.9	------------------------------------------------------------------
00:00:00.9	pump: SetSettings(PumpSettings)
00:00:00.9	pump: GetPumpSideState(A) --> manualMode
00:00:00.9	pump: GetPumpSideState(B) --> manualMode
00:00:00.9	pump: GetPistonPosition(A) --> 505.587187157228
00:00:00.9	pump: GetPistonPosition(B) --> 101.012047469893
00:00:00.9	-------------------------------------------------------
00:00:00.9	--- Optional Refill of Pump Heads:
00:00:00.9	--- initiated by channel A:     False, B: False
00:00:00.9	pump: GetPistonPosition(A) --> 505.587187157228
00:00:00.9	pump: GetPistonPosition(B) --> 101.012047469893
00:00:00.9	--- Piston position channel A:  505.587187157228, B: 101.012047469893
00:00:00.9	-------------------------------------------------------
00:00:00.9	pump: SetDigitalOutput(PO2, False)
00:00:00.9	pump: GetCurrentPressure(A) --> 6.326182
00:00:00.9	pump: GetCurrentPressure(B) --> 6.093479
00:00:00.9	pump: GetSettings() --> PumpSettings
00:00:00.9	pump: GetSetManualValvePosition(A) --> 240
00:00:00.9	pump: GetSetManualValvePosition(B) --> 240
00:00:00.9	-------------------------------------------------------
00:00:00.9	--- Reduce pressure ---
00:00:00.9	-------------------------------------------------------
00:00:00.9	--- Pressure channel A: 6.32618236541748
00:00:00.9	--- Pressure channel B: 6.09347915649414
00:00:00.9	pump: GetSettings() --> PumpSettings
00:00:00.9	------------------------------------------------------------------
00:00:00.9	PID settings changed:
00:00:00.9	------------------------------------------------------------------
00:00:00.9	P: 250 : True; Pmin: -5500 : True; Pmax: 32000 : True
00:00:00.9	I: 50 : True; Imin: -20000 : True; Imax: 20000 : True; IWindow: 50 : True
00:00:00.9	D: 1500 : True; Dmin: -10000 : True; Dmax: 10000 : True
00:00:00.9	Result min: -32000 : True; Result max: 32000 : True
00:00:00.9	------------------------------------------------------------------
00:00:00.9	pump: SetSettings(PumpSettings)
00:00:01.2	--- Set pressure channel A:          10
00:00:01.2	--- Set pressure channel B:          5
00:00:01.2	--- Reduce pressure flow controlled: True
00:00:02.4	--------------------------------------------------------
00:00:02.4	--- Log PID parameter called from: reducePressure
00:00:02.4	--------------------------------------------------------
00:00:02.4	--- P: 175; Pmin: -5000; Pmax: 5000
00:00:02.4	--- I: 5; Imin: -200; Imax: 1000; IWindow: 10
00:00:02.4	--- D: 0; Dmin: -1000; Dmax: 1000
00:00:02.4	--- Result min: -6000; Result max: 8000
00:00:02.4	--------------------------------------------------------
00:00:02.4	--------------------------------------------------------
00:00:02.4	--- Log instrument settings called from: reducePressure
00:00:02.4	--------------------------------------------------------
00:00:02.4	--- HomeSpeed: 2000; PurgeSpeed: 2000
00:00:02.4	--- PressureTrackingExcessFactor: 96
00:00:02.4	--- FlowResistanceA: 300; FlowResistanceB: 100; FlowResistanceOut: 150
00:00:02.4	--- FC_FlowFilterDepth: 5; FC_PressureFilterDepth: 0
00:00:02.4	--- PC_OutputFilterDepth: 0
00:00:02.4	--- CompressibilityFactorB: 2.29999995231628; PressurePIDNegativeResultDivisor: 1
00:00:02.4	--- ExternalZeroPressureOffsetA: 130.944732666016; ExternalZeroPressureOffsetB: 128.061676025391
00:00:02.4	--- ExternalPressureScalingFactorA: 0.0197199992835522; ExternalPressureScalingFactorB: 0.0197199992835522
00:00:02.4	--------------------------------------------------------
00:00:02.4	--- Final pressure channel A: 6.46422243118286
00:00:02.4	--- Final pressure channel B: 0
00:00:02.4	-------------------------------------------------------
00:00:02.4	pump: GetSetManualValvePosition(A) --> 240
00:00:02.4	pump: GetCurrentPressure(A) --> 6.464222
00:00:02.4	pump: SetManualValvePosition(A, 0, True)
00:00:03.0	pump: GetSetManualValvePosition(B) --> 240
00:00:03.0	pump: GetCurrentPressure(B) --> 0
00:00:03.0	pump: SetManualValvePosition(B, 180, False)
00:00:03.4	pump: GetSettings() --> PumpSettings
00:00:03.4	------------------------------------------------------------------
00:00:03.4	PID settings changed:
00:00:03.4	------------------------------------------------------------------
00:00:03.4	P: 250 : True; Pmin: -10000 : True; Pmax: 10000 : True
00:00:03.4	I: 20 : True; Imin: -10000 : True; Imax: 20000 : True; IWindow: 10 : True
00:00:03.4	D: 1500 : True; Dmin: -10000 : True; Dmax: 10000 : True
00:00:03.4	Result min: -10000 : True; Result max: 32000 : True
00:00:03.4	------------------------------------------------------------------
00:00:03.4	pump: SetSettings(PumpSettings)
00:00:03.4	status: SetStatus(equilibrate separation-column, this finishes at about: 4.85 min., )
00:00:03.7	autosampler-none: MoveValveDrive(Valve Drive 2, 180 deg)
00:00:03.7	--- MaxPressureLimitA: 800 bar ---
00:00:03.7	pump: GetCurrentPressure(A) --> 6.641702
00:00:03.7	pump: GetCurrentPressure() --> 6.641702
00:00:03.7	pump: GetSettings() --> PumpSettings
00:00:03.7	pump: GetSetManualValvePosition(A) --> 0
00:00:03.7	-------------------------------------------------------
00:00:03.7	--- Reduce pressure ---
00:00:03.7	-------------------------------------------------------
00:00:03.7	--- Pressure channel A: 6.64170217514038
00:00:03.7	--- Pressure channel B: 6.64170217514038
00:00:03.7	pump: Manualmode_Pump_constantSpeed(A, 0)
00:00:03.7	pump: GetSetPistonSpeed(A) --> 0
00:00:03.7	pump: Manualmode_Pump_constantSpeed(, 0)
00:00:03.7	pump: GetSetPistonSpeed() --> 0
00:00:03.7	pump: IsIdle() --> True
00:00:03.7	-------------------------------------------------------
00:00:03.8	pump: SetMaxPressureLimit(A, 820)
00:00:03.8	pump: GetMaxPressureLimit(A) --> 820
00:00:03.8	-------------------------------------------------------
00:00:03.8	--- Separation Column Equilibration:
00:00:03.8	-------------------------------------------------------
00:00:03.8	--- ovenTemp [C]:                                 50
00:00:03.8	--- Separator Unity-Flow @ ovenTemp [ul/min/bar]: 0.00058605984562038
00:00:03.8	--- Separator Volume [ul]:                        0.463875790256618
00:00:03.8	--- Separator Equilibration Volume Multiplier:    4
00:00:03.8	--- Separator Equilibration Pressure [bar]:       800
00:00:03.8	--- Separator Equilibration Flow [ul/min]:        0.457694594849779
00:00:03.8	--- Separator Equilibration Volume [ul]:          1.85550316102647
00:00:03.8	--- Separator Equilibration Time [min]:           4.05402026133927
00:00:03.8	--- max. Volume (10*V+2ul/min*t) [ul]:            26.6630721329433
00:00:03.8	--- Separator Equilibration Time Out [min]:       40.5402026133927
00:00:03.8	-------------------------------------------------------
00:00:03.8	pump: Manualmode_Pump_constantPressure(A, 800)
00:00:03.8	pump: GetPistonPosition(A) --> 505.587187157228
00:00:03.8	--- Injection delay: 237.164855895902s
00:00:25.8	pump: GetPistonPosition(A) --> 578.707337290509
00:00:25.8	pump: GetPistonPosition(A) --> 578.707337290509
00:00:25.8	pump: GetPistonPosition(A) --> 578.707337290509
00:04:01.0	status: SetStatus(aspirate & inject, )
00:04:01.5	autosampler-left: LeaveObject()
00:04:05.6	autosampler-left: MoveToObject(LCMS Wash 1, 3)
00:04:06.4	autosampler-left: PenetrateObject(LCMS Wash 1, 3)
00:04:06.8	autosampler-left: EmptySyringe()
00:04:07.4	autosampler-left: LeaveObject()
00:04:08.1	Cleaning syringe and needle exterior
00:04:23.9	autosampler-left: CleanSyringe(LCMS Wash 1, 1, 30 mm, 40 uL/s, 500 ms, 30 mm, 40 uL/s, 80 %, 2)
00:04:23.9	autosampler-left: ChangeTool(LCP 1) --> False
00:04:27.0	autosampler-left: MoveToObject(Item Position 3, 1, True, True, True)
00:04:29.4	pump: GetPistonPosition(A) --> 582.263201295353
00:04:29.4	pump: GetCurrentPressure(A) --> 800.0364
00:04:29.4	--- act. pressure [bar]:                 800.036437988281
00:04:29.4	--- used volume [uL]:                    3.55586400484322
00:04:29.4	--- average flow (incl. leaks) [uL/min]: 0.875839358820241
00:04:29.4	--- act. equilibration time [min]:       4.05995000000112
00:04:29.4	-------------------------------------------------------
00:04:29.4	status: RemoveStatus(equilibrate separation-column, this finishes at about: 4.85 min.)
00:04:29.4	status: SetStatus(equilibrate trap-column, this finishes at about: 5.62 min., )
00:04:29.5	--- MaxPressureLimitA: 217.5 bar ---
00:04:29.5	pump: GetCurrentPressure(A) --> 799.9378
00:04:29.5	pump: GetCurrentPressure() --> 799.9378
00:04:29.5	pump: GetSettings() --> PumpSettings
00:04:29.5	pump: GetSetManualValvePosition(A) --> 0
00:04:29.5	-------------------------------------------------------
00:04:29.5	--- Reduce pressure ---
00:04:29.5	-------------------------------------------------------
00:04:29.5	--- Pressure channel A: 799.937805175781
00:04:29.5	--- Pressure channel B: 799.937805175781
00:04:29.5	pump: GetSettings() --> PumpSettings
00:04:29.5	------------------------------------------------------------------
00:04:29.5	PID settings changed:
00:04:29.5	------------------------------------------------------------------
00:04:29.5	P: 250 : True; Pmin: -32000 : True; Pmax: 32000 : True
00:04:29.5	I: 20 : True; Imin: -20000 : True; Imax: 20000 : True; IWindow: 100 : True
00:04:29.5	D: 1500 : True; Dmin: -10000 : True; Dmax: 10000 : True
00:04:29.5	Result min: -32000 : True; Result max: 32000 : True
00:04:29.5	------------------------------------------------------------------
00:04:29.5	pump: SetSettings(PumpSettings)
00:04:29.6	--- Set pressure channel A:          217.5
00:04:29.6	--- Set pressure channel B:          217.5
00:04:29.6	--- Reduce pressure flow controlled: False
00:04:30.1	autosampler-left: PenetrateWithBottomSense(Item Position 3, 1, 1 mm)
00:04:31.1	autosampler-left: AspirateSyringe(2 uL, 5 uL/s, 500 ms)
00:04:32.3	autosampler-left: LeaveObject()
00:04:33.0	autosampler-left: AspirateSyringe(0.5 uL, 5 uL/s, 500 ms)
00:04:43.3	autosampler-left: MoveToObject(Rack 2, 12, True, True, True)
00:04:43.6	--------------------------------------------------------
00:04:43.6	--- Log PID parameter called from: reducePressure
00:04:43.6	--------------------------------------------------------
00:04:43.6	--- P: 250; Pmin: -10000; Pmax: 10000
00:04:43.6	--- I: 20; Imin: -10000; Imax: 20000; IWindow: 10
00:04:43.6	--- D: 1500; Dmin: -10000; Dmax: 10000
00:04:43.6	--- Result min: -10000; Result max: 32000
00:04:43.6	--------------------------------------------------------
00:04:43.6	--------------------------------------------------------
00:04:43.6	--- Log instrument settings called from: reducePressure
00:04:43.6	--------------------------------------------------------
00:04:43.6	--- HomeSpeed: 2000; PurgeSpeed: 2000
00:04:43.6	--- PressureTrackingExcessFactor: 96
00:04:43.6	--- FlowResistanceA: 300; FlowResistanceB: 100; FlowResistanceOut: 150
00:04:43.6	--- FC_FlowFilterDepth: 5; FC_PressureFilterDepth: 0
00:04:43.6	--- PC_OutputFilterDepth: 0
00:04:43.6	--- CompressibilityFactorB: 2.29999995231628; PressurePIDNegativeResultDivisor: 1
00:04:43.6	--- ExternalZeroPressureOffsetA: 130.944732666016; ExternalZeroPressureOffsetB: 128.061676025391
00:04:43.6	--- ExternalPressureScalingFactorA: 0.0197199992835522; ExternalPressureScalingFactorB: 0.0197199992835522
00:04:43.6	--------------------------------------------------------
00:04:43.6	--- Final pressure channel A: 215.673690795898
00:04:43.6	--- Final pressure channel B: 215.673690795898
00:04:43.6	-------------------------------------------------------
00:04:43.7	pump: SetMaxPressureLimit(A, 237.5)
00:04:43.7	pump: GetMaxPressureLimit(A) --> 237.5
00:04:44.3	autosampler-none: MoveValveDrive(Valve Drive 2, 60 deg)
00:04:44.3	-------------------------------------------------------
00:04:44.3	--- Trap Column Equilibration
00:04:44.3	-------------------------------------------------------
00:04:44.3	--- Trap Temperature: fixed to 20 C
00:04:44.3	--- Trap Unity-Flow  @ 20C [ul/bar]:      2.78838695282287
00:04:44.3	--- Trap Volume [ul]:                     0.148440252882118
00:04:44.3	--- Trap Equilibration Volume Multiplier: 10
00:04:44.3	--- Trap Equilibration Pressure [bar]:    217.5
00:04:44.3	--- Trap Equilibration Flow [ul/min]:     9.99557465783688
00:04:44.3	--- Trap Equilibration Volume (+5) [ul]:  6.48440252882118
00:04:44.3	--- Trap Equilibration Time [min]:        0.648727336925764
00:04:44.3	--- max. Volume (10*V+2ul/min*t) [ul]:    66.1414799620633
00:04:44.3	-------------------------------------------------------
00:04:44.3	pump: Manualmode_Pump_constantPressure(A, 217.5)
00:04:44.3	pump: GetPistonPosition(A) --> 530.687884140879
00:04:46.8	autosampler-left: PenetrateWithBottomSense(Rack 2, 12, 1 mm)
00:04:48.5	autosampler-left: AspirateSyringe(5 uL, 5 uL/s, 500 ms)
00:04:56.1	autosampler-left: LeaveObject()
00:04:57.0	autosampler-left: AspirateSyringe(1 uL, 5 uL/s, 500 ms)
00:05:00.5	autosampler-left: MoveToObject(LCMS Wash 1, 1, True, True, True)
00:05:01.5	autosampler-left: PenetrateObject(LCMS Wash 1, 1, 30 mm)
00:05:06.5	autosampler-left: Wait(5 s)
00:05:07.3	autosampler-left: LeaveObject()
00:05:09.3	pump: GetPistonPosition(A) --> 534.075987098021
00:05:09.3	pump: GetPistonPosition(A) --> 534.075987098021
00:05:09.3	pump: GetPistonPosition(A) --> 534.075987098021
00:05:10.3	autosampler-left: MoveToObject(LCInjector1)
00:05:11.3	autosampler-left: DispenseSyringe(0.75 uL, 1 uL/s)
00:05:13.6	autosampler-left: PenetrateWithConstForce(LCInjector1)
00:05:13.8	autosampler-left: MoveValveDrive(Valve Drive 1, 0 deg)
00:05:15.1	autosampler-left: Wait(1000 ms)
00:05:22.0	autosampler-left: DispenseSyringe(6.75 uL, 1 uL/s)
00:05:23.0	autosampler-left: Wait(1000 ms)
00:05:23.3	status: RemoveStatus(aspirate & inject)
00:05:48.3	pump: GetPistonPosition(A) --> 539.18589226884
00:05:48.3	pump: GetPistonPosition(A) --> 539.18589226884
00:05:59.3	pump: GetPistonPosition(A) --> 540.61301309161
00:05:59.3	pump: GetCurrentPressure(A) --> 217.4091
00:05:59.3	-------------------------------------------------------
00:05:59.3	--- Finished: Trap Column Equilibration (const_pressure_load_and_equilibration)
00:05:59.3	-------------------------------------------------------
00:05:59.3	--- act. pressure [bar]:           217.409057617188
00:05:59.3	--- used volume [uL]:              6.53702599358962
00:05:59.3	--- average flow [uL/min]:         7.83878726546386
00:05:59.3	--- act. equilibration time [min]: 0.833933333334668
00:05:59.3	-------------------------------------------------------
00:05:59.3	status: RemoveStatus(equilibrate trap-column, this finishes at about: 5.62 min.)
00:06:01.3	--- MaxPressureLimitA: 217.5 bar ---
00:06:01.3	pump: GetCurrentPressure(A) --> 217.4682
00:06:01.3	pump: GetCurrentPressure() --> 217.4682
00:06:01.3	pump: GetSettings() --> PumpSettings
00:06:01.3	pump: GetSetManualValvePosition(A) --> 0
00:06:01.3	-------------------------------------------------------
00:06:01.3	--- Reduce pressure ---
00:06:01.3	-------------------------------------------------------
00:06:01.3	--- Pressure channel A: 217.468215942383
00:06:01.3	--- Pressure channel B: 217.468215942383
00:06:01.3	pump: Manualmode_Pump_constantSpeed(A, 0)
00:06:01.3	pump: GetSetPistonSpeed(A) --> 0
00:06:01.3	pump: Manualmode_Pump_constantSpeed(, 0)
00:06:01.3	pump: GetSetPistonSpeed() --> 0
00:06:01.3	pump: IsIdle() --> False
00:06:01.3	-------------------------------------------------------
00:06:01.4	pump: SetMaxPressureLimit(A, 237.5)
00:06:01.4	pump: GetMaxPressureLimit(A) --> 237.5
00:06:01.4	status: SetStatus(load trap-column, this finishes at about: 8.29 min., )
00:06:01.9	autosampler-left: MoveValveDrive(Valve Drive 1, 60 deg)
00:06:02.7	-------------------------------------------------------
00:06:02.7	--- Sample Loading on Trap Column
00:06:02.7	-------------------------------------------------------
00:06:02.7	--- Trap Temperature: fixed to 20 C
00:06:02.7	--- Trap Unity-Flow [ul/bar]:          2.78838695282287
00:06:02.7	--- Trap Volume [ul]:                  0.148440252882118
00:06:02.7	--- Sample Volume [ul]:                5
00:06:02.7	--- Sample Volume Multiplier:          4
00:06:02.7	--- Trap Loading Pressure [bar]:       217.5
00:06:02.7	--- Trap Loading Flow [uL/min]:        9.99557465783688
00:06:02.7	--- Trap Loading Volume (+2) [uL]:     22
00:06:02.7	--- Trap Loading Time [min]:           2.200974006307
00:06:02.7	--- max. Volume (10*V+2ul/min*t) [ul]: 224.401948012614
00:06:02.7	-------------------------------------------------------
00:06:02.7	pump: Manualmode_Pump_constantPressure(A, 217.5)
00:06:02.7	pump: GetPistonPosition(A) --> 540.885703333942
00:06:02.7	autosampler-left: EmptySyringe(5 uL/s)
00:06:21.0	pump: GetPistonPosition(A) --> 543.884458241548
00:06:21.0	pump: GetPistonPosition(A) --> 543.884458241548
00:06:21.0	pump: GetPistonPosition(A) --> 543.884458241548
00:07:01.4	autosampler-left: CleanInjector(LCInjector1, LCP 1, 200 uL, 0 mm, 5 uL/s, 5 s, 20 mm, 40 uL/s, 1000 ms, 2, False)
00:08:02.7	autosampler-left: CleanInjector(LCInjector1, LCP 1, 200 uL, 0 mm, 5 uL/s, 5 s, 20 mm, 40 uL/s, 1000 ms, 1, True)
00:08:18.4	autosampler-left: CleanSyringe(LCMS Wash 1, 2, 30 mm, 40 uL/s, 500 ms, 30 mm, 40 uL/s, 80 %, 2)
00:08:33.1	pump: GetPistonPosition(A) --> 561.235474467325
00:08:33.1	pump: GetPistonPosition(A) --> 561.235474467325
00:08:38.7	autosampler-left: CleanSyringe(LCMS Wash 1, 1, 30 mm, 40 uL/s, 500 ms, 30 mm, 40 uL/s, 80 %, 3)
00:08:42.1	autosampler-left: MoveToHome()
00:09:08.6	pump: GetPistonPosition(A) --> 565.901367876437
00:09:08.6	pump: GetCurrentPressure(A) --> 217.5668
00:09:08.6	-------------------------------------------------------
00:09:08.6	--- Finished: Sample Loading on Trap Column (const_pressure_load_and_equilibration)
00:09:08.6	-------------------------------------------------------
00:09:08.6	--- act. pressure [bar]:     217.566818237305
00:09:08.6	--- used volume [uL]:        22.0169096348881
00:09:08.6	--- average flow [uL/min]:   7.88091406911676
00:09:08.6	--- act. loading time [min]: 2.79369999999957
00:09:08.6	-------------------------------------------------------
00:09:08.6	--- Separator: Name: IonOpticks 25 cm, Func: Separator, ColDia: 0.075 mm, Len: 250 mm, PartDia: 1.6 µm, Porosity: 42 % ---
00:09:08.6	--- Trap: Name: Thermo Trap Cartridge 5mm, Func: Separator, ColDia: 0.3 mm, Len: 5 mm, PartDia: 5 µm, Porosity: 42 % ---
00:09:08.6	--- MaxPressureLimitAB: 1000 bar ---
00:09:08.6	pump: GetCurrentPressure(A) --> 217.5668
00:09:08.6	pump: GetCurrentPressure(B) --> 0
00:09:08.6	pump: GetSettings() --> PumpSettings
00:09:08.6	pump: GetSetManualValvePosition(A) --> 0
00:09:08.6	-------------------------------------------------------
00:09:08.6	--- Reduce pressure ---
00:09:08.6	-------------------------------------------------------
00:09:08.6	--- Pressure channel A: 217.566818237305
00:09:08.6	--- Pressure channel B: 0
00:09:08.6	pump: Manualmode_Pump_constantSpeed(A, 0)
00:09:08.6	pump: GetSetPistonSpeed(A) --> 0
00:09:08.6	pump: Manualmode_Pump_constantSpeed(B, 0)
00:09:08.6	pump: GetSetPistonSpeed(B) --> 0
00:09:08.6	pump: IsIdle() --> False
00:09:08.6	-------------------------------------------------------
00:09:08.7	pump: SetMaxPressureLimit(A, 1020)
00:09:08.7	pump: GetMaxPressureLimit(A) --> 1020
00:09:08.8	pump: SetMaxPressureLimit(B, 1020)
00:09:08.8	pump: GetMaxPressureLimit(B) --> 1020
00:09:08.8	status: RemoveStatus(load trap-column, this finishes at about: 8.29 min.)
00:09:08.8	pump: GetSettings() --> PumpSettings
00:09:08.8	------------------------------------------------------------------
00:09:08.8	PID settings changed:
00:09:08.8	------------------------------------------------------------------
00:09:08.8	P: 100 : True; Pmin: -10000 : True; Pmax: 10000 : True
00:09:08.8	I: 100 : True; Imin: -1000 : True; Imax: 1000 : True; IWindow: 100 : True
00:09:08.8	D: 5000 : True; Dmin: -32000 : True; Dmax: 32000 : True
00:09:08.8	Result min: -5000 : True; Result max: 25000 : True
00:09:08.8	------------------------------------------------------------------
00:09:08.8	pump: SetSettings(PumpSettings)
00:09:08.8	pump: GetSettings() --> PumpSettings
00:09:08.8	------------------------------------------------------------------
00:09:08.8	PID settings changed:
00:09:08.8	------------------------------------------------------------------
00:09:08.8	P: 100 : False; Pmin: -10000 : False; Pmax: 10000 : False
00:09:08.8	I: 100 : False; Imin: -1000 : False; Imax: 1000 : False; IWindow: 2 : True
00:09:08.8	D: 5000 : False; Dmin: -32000 : False; Dmax: 32000 : False
00:09:08.8	Result min: -5000 : False; Result max: 25000 : False
00:09:08.8	------------------------------------------------------------------
00:09:08.8	pump: SetSettings(PumpSettings)
00:09:08.8	status: SetStatus(preparing gradient start, )
00:09:08.9	--- Starting conditioning paths ---
00:09:08.9	pump: GetSettings() --> PumpSettings
00:09:08.9	------------------------------------------------------------------
00:09:08.9	PID settings changed:
00:09:08.9	------------------------------------------------------------------
00:09:08.9	P: 100 : True; Pmin: -10000 : True; Pmax: 10000 : True
00:09:08.9	I: 100 : True; Imin: -1000 : True; Imax: 1000 : True; IWindow: 100 : True
00:09:08.9	D: 5000 : True; Dmin: -32000 : True; Dmax: 32000 : True
00:09:08.9	Result min: -5000 : True; Result max: 25000 : True
00:09:08.9	------------------------------------------------------------------
00:09:08.9	pump: SetSettings(PumpSettings)
00:09:08.9	pump: Manualmode_Pump_constantPressure(A, 0)
00:09:08.9	pump: GetTargetPressure(A) --> 0
00:09:08.9	pump: GetCurrentPressure(A) --> 217.5077
00:09:09.9	pump: GetCurrentPressure(A) --> 201.0415
00:09:10.9	pump: GetCurrentPressure(A) --> 178.324
00:09:11.9	pump: GetCurrentPressure(A) --> 156.1193
00:09:12.9	pump: GetCurrentPressure(A) --> 133.816
00:09:13.9	pump: GetCurrentPressure(A) --> 111.2366
00:09:14.9	pump: GetCurrentPressure(A) --> 89.24878
00:09:15.9	pump: GetCurrentPressure(A) --> 68.10894
00:09:16.9	pump: GetCurrentPressure(A) --> 49.19746
00:09:17.9	pump: GetCurrentPressure(A) --> 28.5309
00:09:18.9	pump: GetCurrentPressure(A) --> 16.12702
00:09:19.9	pump: GetCurrentPressure(A) --> 10.64486
00:09:20.1	autosampler-none: MoveValveDrive(Valve Drive 2, 0 deg)
00:09:20.1	pump: SetManualValvePosition(A, 240, False)
00:09:20.7	pump: SetManualValvePosition(B, 240, False)
00:09:22.7	--- Set constant binary flow ---
00:09:22.7	pump: Manualmode_Pump_constantFlow_binary(1, 1)
00:09:22.7	pump: GetSetFlow(A) --> 1
00:09:22.7	pump: GetSetFlow(B) --> 1
00:09:22.7	

00:09:22.7	--- Starting spline flow reduction B/A ---
00:09:22.8	pump: GetSetExternalTemperature() --> 50
00:09:22.8	--- Oven temperatur: 50
00:09:22.8	--- Separator: Name: IonOpticks 25 cm, Func: Separator, ColDia: 0.075 mm, Len: 250 mm, PartDia: 1.6 µm, Porosity: 42 %
00:09:22.8	--- Spline fitting time: 97.6577595902957 s
00:09:22.8	--- Spline fitting time adapted: 10 s
00:09:22.8	--- y = 0x + 1
00:09:22.8	--- p0.Time -1, p0.Flow 1
00:09:22.8	--- p1.Time 0, p1.Flow 1
00:09:22.8	--- p2.Time 9, p2.Flow 1
00:09:22.8	--- p3.Time 10, p3.Flow 1, p3.Mix 0
00:09:22.8	--- p4.Time 40, p4.Flow 1, p4.Mix 0
00:09:22.8	--- Strategy function end
00:09:22.8	--- y = 0x + 0
00:09:22.8	--- p0.Time -1, p0.Flow 1
00:09:22.8	--- p1.Time 0, p1.Flow 1
00:09:22.8	--- p2.Time 9, p2.Flow 0
00:09:22.8	--- p3.Time 10, p3.Flow 0, p3.Mix 0
00:09:22.8	--- p4.Time 40, p4.Flow 0, p4.Mix 0
00:09:22.8	--- Strategy function end
00:09:22.8	xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx
00:09:22.8	XXXXX Time 0, Flow 2, Mix 50
00:09:22.8	XXXXX Time 1, Flow 1.94545, Mix 48.59813
00:09:22.8	XXXXX Time 2, Flow 1.84848, Mix 45.90163
00:09:22.8	XXXXX Time 3, Flow 1.72121, Mix 41.9014
00:09:22.8	XXXXX Time 4, Flow 1.57575, Mix 36.53846
00:09:22.8	XXXXX Time 5, Flow 1.42424, Mix 29.78723
00:09:22.8	XXXXX Time 6, Flow 1.27878, Mix 21.80094
00:09:22.8	XXXXX Time 7, Flow 1.15151, Mix 13.15789
00:09:22.8	XXXXX Time 8, Flow 1.05454, Mix 5.17241
00:09:22.8	XXXXX Time 9, Flow 1, Mix 0
00:09:22.8	--- Gradient is created, switching valves A and B
00:09:22.8	pump: GetSetManualValvePosition(A) --> 240
00:09:22.8	pump: GetCurrentPressure(A) --> 4.117542
00:09:22.8	pump: SetManualValvePosition(A, 240, False)
00:09:23.0	pump: GetSetManualValvePosition(B) --> 240
00:09:23.0	pump: GetCurrentPressure(B) --> 0
00:09:23.0	pump: SetManualValvePosition(B, 240, False)
00:09:23.2	--- Valves A and B switched to MixTee, starting gradient
00:09:23.2	--- LoadGradient() ---
00:09:23.2	--- Loading gradient method index: 0
00:09:23.2	pump: LoadMethod(0, ZrData.ZrPumpMethod)
00:09:23.7	pump: GetMethodState(0) --> loaded
00:09:23.7	--- Set gradient start
00:09:23.7	--- StartGradient() ---
00:09:23.7	--- Starting gradient method index: 0
00:09:23.7	pump: SetMethodSignal(0, StartTrunk)
00:09:23.9	pump: GetMethodState(0) --> runningTrunk
00:09:23.9	--- Start GradientSplineFit
00:09:23.9	pump: GetMethodState(0) --> runningTrunk
00:09:24.9	pump: GetCurrentPressure(A) --> 77.61398
00:09:24.9	pump: GetCurrentPressure(B) --> 49.22112
00:09:24.9	--- GradientSplineFit is running
00:09:33.0	--- GradientSplineFit has finished
00:09:33.0	--- End GradientSplineFit
00:09:33.0	--- Finished spline flow reduction B ---
00:09:34.0	pump: GetSetExternalTemperature() --> 50
00:09:34.0	--- Oven temperatur: 50
00:09:34.0	--- Separator: Name: IonOpticks 25 cm, Func: Separator, ColDia: 0.075 mm, Len: 250 mm, PartDia: 1.6 µm, Porosity: 42 %
00:09:34.0	--- Spline fitting time: 97.6577595902957 s
00:09:34.0	--- Spline fitting time adapted: 60 s
00:09:34.0	--- y = 0x + 0.01
00:09:34.0	--- p0.Time -1, p0.Flow 1
00:09:34.0	--- p1.Time 0, p1.Flow 1
00:09:34.0	--- p2.Time 59, p2.Flow 0.01
00:09:34.0	--- p3.Time 60, p3.Flow 0.01, p3.Mix 0
00:09:34.0	--- p4.Time 90, p4.Flow 0.01, p4.Mix 0
00:09:34.0	--- Strategy function end
00:09:34.0	--- y = 0x + 0
00:09:34.0	--- p0.Time -1, p0.Flow 0
00:09:34.0	--- p1.Time 0, p1.Flow 0
00:09:34.0	--- p2.Time 59, p2.Flow 0
00:09:34.0	--- p3.Time 60, p3.Flow 0, p3.Mix 0
00:09:34.0	--- p4.Time 90, p4.Flow 0, p4.Mix 0
00:09:34.0	--- Strategy function end
00:09:34.0	xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx
00:09:34.0	XXXXX Time 0, Flow 1, Mix 0
00:09:34.0	XXXXX Time 1, Flow 0.99837, Mix 0
00:09:34.0	XXXXX Time 2, Flow 0.99518, Mix 0
00:09:34.0	XXXXX Time 3, Flow 0.99048, Mix 0
00:09:34.0	XXXXX Time 4, Flow 0.98432, Mix 0
00:09:34.0	XXXXX Time 5, Flow 0.97675, Mix 0
00:09:34.1	XXXXX Time 6, Flow 0.96784, Mix 0
00:09:34.1	XXXXX Time 7, Flow 0.95763, Mix 0
00:09:34.1	XXXXX Time 8, Flow 0.94619, Mix 0
00:09:34.1	XXXXX Time 9, Flow 0.93356, Mix 0
00:09:34.1	XXXXX Time 10, Flow 0.91981, Mix 0
00:09:34.1	XXXXX Time 11, Flow 0.90498, Mix 0
00:09:34.1	XXXXX Time 12, Flow 0.88914, Mix 0
00:09:34.1	XXXXX Time 13, Flow 0.87233, Mix 0
00:09:34.1	XXXXX Time 14, Flow 0.85462, Mix 0
00:09:34.1	XXXXX Time 15, Flow 0.83605, Mix 0
00:09:34.1	XXXXX Time 16, Flow 0.81668, Mix 0
00:09:34.1	XXXXX Time 17, Flow 0.79658, Mix 0
00:09:34.1	XXXXX Time 18, Flow 0.77578, Mix 0
00:09:34.1	XXXXX Time 19, Flow 0.75435, Mix 0
00:09:34.1	XXXXX Time 20, Flow 0.73235, Mix 0
00:09:34.1	XXXXX Time 21, Flow 0.70982, Mix 0
00:09:34.1	XXXXX Time 22, Flow 0.68682, Mix 0
00:09:34.1	XXXXX Time 23, Flow 0.66341, Mix 0
00:09:34.1	XXXXX Time 24, Flow 0.63964, Mix 0
00:09:34.2	XXXXX Time 25, Flow 0.61558, Mix 0
00:09:34.2	XXXXX Time 26, Flow 0.59126, Mix 0
00:09:34.2	XXXXX Time 27, Flow 0.56675, Mix 0
00:09:34.2	XXXXX Time 28, Flow 0.5421, Mix 0
00:09:34.2	XXXXX Time 29, Flow 0.51737, Mix 0
00:09:34.2	XXXXX Time 30, Flow 0.49262, Mix 0
00:09:34.2	XXXXX Time 31, Flow 0.46789, Mix 0
00:09:34.2	XXXXX Time 32, Flow 0.44324, Mix 0
00:09:34.2	XXXXX Time 33, Flow 0.41873, Mix 0
00:09:34.2	XXXXX Time 34, Flow 0.39441, Mix 0
00:09:34.2	XXXXX Time 35, Flow 0.37035, Mix 0
00:09:34.2	XXXXX Time 36, Flow 0.34658, Mix 0
00:09:34.2	XXXXX Time 37, Flow 0.32317, Mix 0
00:09:34.2	XXXXX Time 38, Flow 0.30017, Mix 0
00:09:34.2	XXXXX Time 39, Flow 0.27764, Mix 0
00:09:34.2	XXXXX Time 40, Flow 0.25564, Mix 0
00:09:34.3	XXXXX Time 41, Flow 0.23421, Mix 0
00:09:34.3	XXXXX Time 42, Flow 0.21341, Mix 0
00:09:34.3	XXXXX Time 43, Flow 0.19331, Mix 0
00:09:34.3	XXXXX Time 44, Flow 0.17394, Mix 0
00:09:34.3	XXXXX Time 45, Flow 0.15537, Mix 0
00:09:34.3	XXXXX Time 46, Flow 0.13766, Mix 0
00:09:34.3	XXXXX Time 47, Flow 0.12085, Mix 0
00:09:34.3	XXXXX Time 48, Flow 0.10501, Mix 0
00:09:34.3	XXXXX Time 49, Flow 0.09018, Mix 0
00:09:34.3	XXXXX Time 50, Flow 0.07643, Mix 0
00:09:34.3	XXXXX Time 51, Flow 0.0638, Mix 0
00:09:34.3	XXXXX Time 52, Flow 0.05236, Mix 0
00:09:34.3	XXXXX Time 53, Flow 0.04215, Mix 0
00:09:34.3	XXXXX Time 54, Flow 0.03324, Mix 0
00:09:34.3	XXXXX Time 55, Flow 0.02567, Mix 0
00:09:34.3	XXXXX Time 56, Flow 0.01951, Mix 0
00:09:34.3	XXXXX Time 57, Flow 0.01481, Mix 0
00:09:34.4	XXXXX Time 58, Flow 0.01162, Mix 0
00:09:34.4	XXXXX Time 59, Flow 0.00999, Mix 0
00:09:34.4	--- Gradient is created, switching valves A and B
00:09:34.4	pump: GetSetManualValvePosition(A) --> 240
00:09:34.4	pump: GetCurrentPressure(A) --> 89.03186
00:09:34.4	pump: SetManualValvePosition(A, 240, False)
00:09:34.6	pump: GetSetManualValvePosition(B) --> 240
00:09:34.6	pump: GetCurrentPressure(B) --> 12.1278
00:09:34.6	pump: SetManualValvePosition(B, 240, False)
00:09:34.8	--- Valves A and B switched to MixTee, starting gradient
00:09:34.8	--- LoadGradient() ---
00:09:34.8	--- Loading gradient method index: 0
00:09:34.8	pump: LoadMethod(0, ZrData.ZrPumpMethod)
00:09:35.3	pump: GetMethodState(0) --> loaded
00:09:35.3	--- Set gradient start
00:09:35.3	--- StartGradient() ---
00:09:35.3	--- Starting gradient method index: 0
00:09:35.3	pump: SetMethodSignal(0, StartTrunk)
00:09:35.5	pump: GetMethodState(0) --> runningTrunk
00:09:35.5	--- Start GradientSplineFit
00:09:35.5	pump: GetMethodState(0) --> runningTrunk
00:09:36.5	pump: GetCurrentPressure(A) --> 90.23478
00:09:36.5	pump: GetCurrentPressure(B) --> 13.54764
00:09:36.5	--- GradientSplineFit is running
00:10:34.5	--- GradientSplineFit has finished
00:10:34.5	--- End GradientSplineFit
00:10:34.5	--- Finished spline flow reduction A ---

00:10:34.5	--- Finished conditioning paths ---
00:10:40.1	autosampler-none: MoveValveDrive(Valve Drive 2, 240 deg)
00:10:40.1	pump: GetSetExternalTemperature() --> 50
00:10:40.1	--- Oven temperatur: 50
00:10:40.1	--- Separator: Name: IonOpticks 25 cm, Func: Separator, ColDia: 0.075 mm, Len: 250 mm, PartDia: 1.6 µm, Porosity: 42 %
00:10:40.1	--- Spline fitting time: 97.6577595902957 s
00:10:40.1	--- Spline fitting time adapted: 53.7117677746626 s
00:10:40.1	--- Press: 541.893115083548; colPress: 511.893115083548, visco: 0.00566684100509561, flow > 0.3
00:10:40.1	--- Flow: 0.3; Mix: 0, calcI: 220.674819441516
00:10:40.1	--- Ki calc: 220.674819441516
00:10:40.1	pump: GetSettings() --> PumpSettings
00:10:40.1	------------------------------------------------------------------
00:10:40.1	PID settings changed:
00:10:40.1	------------------------------------------------------------------
00:10:40.1	P: 200 : True; Pmin: -32000 : True; Pmax: 32000 : True
00:10:40.1	I: 600 : True; Imin: -32000 : True; Imax: 32000 : True; IWindow: 100 : True
00:10:40.1	D: 1000 : True; Dmin: -32000 : True; Dmax: 32000 : True
00:10:40.1	Result min: -32000 : True; Result max: 32000 : True
00:10:40.1	------------------------------------------------------------------
00:10:40.1	pump: SetSettings(PumpSettings)
00:10:40.1	pump: GetSettings() --> PumpSettings
00:10:40.1	------------------------------------------------------------------
00:10:40.1	PID settings changed:
00:10:40.1	------------------------------------------------------------------
00:10:40.1	P: 200 : False; Pmin: -32000 : False; Pmax: 32000 : False
00:10:40.1	I: 220 : True; Imin: -32000 : False; Imax: 32000 : False; IWindow: 100 : False
00:10:40.1	D: 1000 : False; Dmin: -32000 : False; Dmax: 32000 : False
00:10:40.1	Result min: -32000 : False; Result max: 32000 : False
00:10:40.1	------------------------------------------------------------------
00:10:40.1	pump: SetSettings(PumpSettings)
00:10:40.1	--- y = -5.33333333333333E-05x + 0.302864627614649
00:10:40.1	--- p0.Time -1, p0.Flow 0.01
00:10:40.1	--- p1.Time 0, p1.Flow 0.01
00:10:40.1	--- p2.Time 52.7117677746626, p2.Flow 0.300053333333333
00:10:40.1	--- p3.Time 53.7117677746626, p3.Flow 0.3, p3.Mix 0
00:10:40.1	--- p4.Time 1853.71176777466, p4.Flow 0.204, p4.Mix 32
00:10:40.1	--- Strategy function end
00:10:40.1	--- y = 5.33333333333333E-05x + -0.00286462761464867
00:10:40.1	--- p0.Time -1, p0.Flow 0
00:10:40.1	--- p1.Time 0, p1.Flow 0
00:10:40.1	--- p2.Time 52.7117677746626, p2.Flow -5.33333333333336E-05
00:10:40.1	--- p3.Time 53.7117677746626, p3.Flow 0, p3.Mix 0
00:10:40.1	--- p4.Time 1853.71176777466, p4.Flow 0.096, p4.Mix 32
00:10:40.1	--- Strategy function end
00:10:40.1	xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx
00:10:40.1	XXXXX Time 0, Flow 0.01, Mix 0
00:10:40.1	XXXXX Time 1, Flow 0.01059, Mix 0
00:10:40.2	XXXXX Time 2, Flow 0.01175, Mix 0
00:10:40.2	XXXXX Time 3, Flow 0.01347, Mix 0
00:10:40.2	XXXXX Time 4, Flow 0.01571, Mix 0
00:10:40.2	XXXXX Time 5, Flow 0.01845, Mix 0
00:10:40.2	XXXXX Time 6, Flow 0.02168, Mix 0
00:10:40.2	XXXXX Time 7, Flow 0.02537, Mix 0
00:10:40.2	XXXXX Time 8, Flow 0.02949, Mix 0
00:10:40.2	XXXXX Time 9, Flow 0.03402, Mix 0
00:10:40.2	XXXXX Time 10, Flow 0.03894, Mix 0
00:10:40.2	XXXXX Time 11, Flow 0.04424, Mix 0
00:10:40.2	XXXXX Time 12, Flow 0.04988, Mix 0
00:10:40.2	XXXXX Time 13, Flow 0.05584, Mix 0
00:10:40.2	XXXXX Time 14, Flow 0.0621, Mix 0
00:10:40.2	XXXXX Time 15, Flow 0.06864, Mix 0
00:10:40.2	XXXXX Time 16, Flow 0.07544, Mix 0
00:10:40.2	XXXXX Time 17, Flow 0.08247, Mix 0
00:10:40.2	XXXXX Time 18, Flow 0.08971, Mix 0
00:10:40.3	XXXXX Time 19, Flow 0.09713, Mix 0
00:10:40.3	XXXXX Time 20, Flow 0.10472, Mix 0
00:10:40.3	XXXXX Time 21, Flow 0.11246, Mix 0
00:10:40.3	XXXXX Time 22, Flow 0.12031, Mix 0
00:10:40.3	XXXXX Time 23, Flow 0.12826, Mix 0
00:10:40.3	XXXXX Time 24, Flow 0.13629, Mix 0
00:10:40.3	XXXXX Time 25, Flow 0.14437, Mix 0
00:10:40.3	XXXXX Time 26, Flow 0.15248, Mix 0
00:10:40.3	XXXXX Time 27, Flow 0.16059, Mix 0
00:10:40.3	XXXXX Time 28, Flow 0.16869, Mix 0
00:10:40.3	XXXXX Time 29, Flow 0.17675, Mix 0
00:10:40.3	XXXXX Time 30, Flow 0.18476, Mix 0
00:10:40.3	XXXXX Time 31, Flow 0.19267, Mix 0
00:10:40.3	XXXXX Time 32, Flow 0.20049, Mix 0
00:10:40.3	XXXXX Time 33, Flow 0.20817, Mix 0
00:10:40.3	XXXXX Time 34, Flow 0.2157, Mix 0
00:10:40.3	XXXXX Time 35, Flow 0.22306, Mix 0
00:10:40.4	XXXXX Time 36, Flow 0.23022, Mix 0
00:10:40.4	XXXXX Time 37, Flow 0.23717, Mix 0
00:10:40.4	XXXXX Time 38, Flow 0.24387, Mix 0
00:10:40.4	XXXXX Time 39, Flow 0.25031, Mix 0
00:10:40.4	XXXXX Time 40, Flow 0.25646, Mix 0
00:10:40.4	XXXXX Time 41, Flow 0.2623, Mix 0
00:10:40.4	XXXXX Time 42, Flow 0.26781, Mix 0
00:10:40.4	XXXXX Time 43, Flow 0.27297, Mix 0
00:10:40.4	XXXXX Time 44, Flow 0.27775, Mix 0
00:10:40.4	XXXXX Time 45, Flow 0.28213, Mix 0
00:10:40.4	XXXXX Time 46, Flow 0.28609, Mix 0
00:10:40.4	XXXXX Time 47, Flow 0.28961, Mix 0
00:10:40.4	XXXXX Time 48, Flow 0.29265, Mix 0
00:10:40.4	XXXXX Time 49, Flow 0.29521, Mix 0
00:10:40.4	XXXXX Time 50, Flow 0.29726, Mix 0
00:10:40.4	XXXXX Time 51, Flow 0.29877, Mix 0
00:10:40.4	XXXXX Time 52, Flow 0.29972, Mix 0
00:10:40.4	XXXXX Time 53, Flow 0.3001, Mix 0
00:10:40.4	--- Gradient is created, switching valves A and B
00:10:40.4	pump: GetSetManualValvePosition(A) --> 240
00:10:40.4	pump: GetCurrentPressure(A) --> 1.139822
00:10:40.4	pump: SetManualValvePosition(A, 240, False)
00:10:40.6	pump: GetSetManualValvePosition(B) --> 240
00:10:40.6	pump: GetCurrentPressure(B) --> 0
00:10:40.6	pump: SetManualValvePosition(B, 240, False)
00:10:40.8	--- Valves A and B switched to MixTee, starting gradient
00:10:40.8	--- LoadGradient() ---
00:10:40.8	--- Loading gradient method index: 0
00:10:40.8	pump: LoadMethod(0, ZrData.ZrPumpMethod)
00:10:41.3	pump: GetMethodState(0) --> loaded
00:10:41.3	--- Set gradient start
00:10:41.3	--- StartGradient() ---
00:10:41.3	--- Starting gradient method index: 0
00:10:41.3	pump: SetMethodSignal(0, StartTrunk)
00:10:41.5	pump: GetMethodState(0) --> runningTrunk
00:10:41.5	--- Start GradientSplineFit
00:10:41.5	pump: GetMethodState(0) --> runningTrunk
00:10:42.5	pump: GetCurrentPressure(A) --> 0.2327026
00:10:42.5	pump: GetCurrentPressure(B) --> 0
00:10:42.5	--- GradientSplineFit is running
00:11:34.6	--- GradientSplineFit has finished
00:11:34.6	--- End GradientSplineFit
00:11:34.6	status: RemoveStatus(preparing gradient start)
00:11:34.6	Setting signal "lc-ready"
00:11:34.6	Waiting for signal "ms-start"...
00:11:34.7	--- LoadGradient() ---
00:11:34.7	--- Loading gradient method index: 0
00:11:34.7	pump: LoadMethod(0, ZrData.ZrPumpMethod)
00:11:35.2	pump: GetMethodState(0) --> loaded
00:11:35.2	--- Set gradient start
00:11:35.2	--- StartGradient() ---
00:11:35.2	--- Starting gradient method index: 0
00:11:35.2	pump: SetMethodSignal(0, StartTrunk)
00:11:35.4	pump: GetMethodState(0) --> runningTrunk
00:11:35.5	autosampler-none: SetSignal(Output Signal 1)
00:11:35.5	status: SetStatus(wash, )
00:11:39.1	status: SetStatus(elution, )
00:11:40.8	autosampler-left: MoveToObject(LCInjector1)
00:11:43.4	autosampler-left: PenetrateWithConstForce(LCInjector1)
00:11:43.6	autosampler-left: MoveValveDrive(Valve Drive 1, 0 deg)
00:12:13.0	autosampler-left: CleanInjector(LCInjector1, LCP 1, 100 uL, 0 mm, 5 uL/s, 5 s, 20 mm, 40 uL/s, 1000 ms, 2, False)
00:13:43.0	autosampler-left: CleanInjector(LCInjector1, LCP 1, 300 uL, 0 mm, 5 uL/s, 5 s, 20 mm, 40 uL/s, 1000 ms, 1, True)
00:13:43.2	autosampler-left: MoveValveDrive(Valve Drive 1, 60 deg)
00:13:46.4	autosampler-left: MoveToHome()
01:11:36.1	Setting signal "lc-done"
01:11:36.1	status: RemoveStatus(elution)
01:11:36.1	status: RemoveStatus(wash)
01:11:36.1	pump: GetCurrentPressure(A) --> 243.1042
01:11:36.1	pump: GetCurrentPressure(B) --> 251.2722
01:11:36.1	pump: GetSettings() --> PumpSettings
01:11:36.1	pump: GetSetManualValvePosition(A) --> 240
01:11:36.1	pump: GetSetManualValvePosition(B) --> 240
01:11:36.1	-------------------------------------------------------
01:11:36.1	--- Reduce pressure ---
01:11:36.1	-------------------------------------------------------
01:11:36.1	--- Pressure channel A: 243.104217529297
01:11:36.1	--- Pressure channel B: 251.272232055664
01:11:36.1	pump: GetSettings() --> PumpSettings
01:11:36.1	------------------------------------------------------------------
01:11:36.1	PID settings changed:
01:11:36.1	------------------------------------------------------------------
01:11:36.1	P: 250 : True; Pmin: -5500 : True; Pmax: 32000 : True
01:11:36.1	I: 50 : True; Imin: -20000 : True; Imax: 20000 : True; IWindow: 50 : True
01:11:36.1	D: 1500 : True; Dmin: -10000 : True; Dmax: 10000 : True
01:11:36.1	Result min: -32000 : True; Result max: 32000 : True
01:11:36.1	------------------------------------------------------------------
01:11:36.1	pump: SetSettings(PumpSettings)
01:11:36.3	--- Set pressure channel A:          10
01:11:36.3	--- Set pressure channel B:          5
01:11:36.3	--- Reduce pressure flow controlled: True
01:12:06.6	--------------------------------------------------------
01:12:06.6	--- Log PID parameter called from: reducePressure
01:12:06.6	--------------------------------------------------------
01:12:06.6	--- P: 200; Pmin: -32000; Pmax: 32000
01:12:06.6	--- I: 220; Imin: -32000; Imax: 32000; IWindow: 100
01:12:06.6	--- D: 1000; Dmin: -32000; Dmax: 32000
01:12:06.6	--- Result min: -32000; Result max: 32000
01:12:06.6	--------------------------------------------------------
01:12:06.6	--------------------------------------------------------
01:12:06.6	--- Log instrument settings called from: reducePressure
01:12:06.6	--------------------------------------------------------
01:12:06.6	--- HomeSpeed: 2000; PurgeSpeed: 2000
01:12:06.6	--- PressureTrackingExcessFactor: 96
01:12:06.6	--- FlowResistanceA: 300; FlowResistanceB: 100; FlowResistanceOut: 150
01:12:06.6	--- FC_FlowFilterDepth: 5; FC_PressureFilterDepth: 0
01:12:06.6	--- PC_OutputFilterDepth: 0
01:12:06.6	--- CompressibilityFactorB: 2.29999995231628; PressurePIDNegativeResultDivisor: 1
01:12:06.6	--- ExternalZeroPressureOffsetA: 130.944732666016; ExternalZeroPressureOffsetB: 128.061676025391
01:12:06.6	--- ExternalPressureScalingFactorA: 0.0197199992835522; ExternalPressureScalingFactorB: 0.0197199992835522
01:12:06.6	--------------------------------------------------------
01:12:06.6	--- Final pressure channel A: 5.67542219161987
01:12:06.6	--- Final pressure channel B: 4.41727924346924
01:12:06.6	-------------------------------------------------------
01:12:06.9	autosampler-left: MoveValveDrive(Valve Drive 2, 0 deg)
01:12:07.1	pump: Manualmode_Pump_constantSpeed(A, 0)
01:12:07.1	pump: GetSetPistonSpeed(A) --> 0
01:12:07.1	pump: Manualmode_Pump_constantSpeed(B, 0)
01:12:07.1	pump: GetSetPistonSpeed(B) --> 0
01:12:07.1	pump: IsIdle() --> True
01:12:07.6	autosampler-left: MoveValveDrive(Valve Drive 1, 60 deg)
01:12:09.5	autosampler-left: MoveToHome()
01:12:09.6	pump: IsIdle() --> True
01:12:09.6	COMPLETED